(Circular) (Six-base) MAPSORT of: pF1KE0981.seq Check: 2346 from: 1 to: 4853 pF1KE0981 4853 bp With 182 enzymes: SgfI * April 24, 2009 13:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 587 587 Size: 4853 AccI GT'mk_AC Cuts at: 595 1676 1891 595 Size: 1081 215 3557 Fragments arranged by size: 3557 1081 215 Acc65I G'GTAC_C Cuts at: 1869 1869 Size: 4853 AclI AA'CG_TT Cuts at: 1202 4693 1202 Size: 3491 1362 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 177 400 873 931 2678 3110 3857 4125 177 Size: 223 473 58 1747 432 747 268 905 Fragments arranged by size: 1747 905 747 473 432 268 223 58 AfeI AGC'GCT Cuts at: 967 1492 967 Size: 525 4328 AflIII A'CryG_T Cuts at: 2205 3309 4286 2205 Size: 1104 977 2772 Fragments arranged by size: 2772 1104 977 AgeI A'CCGG_T Cuts at: 117 3241 117 Size: 3124 1729 AleI CACnn'nnGTG Cuts at: 243 778 243 Size: 535 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1057 1314 1057 Size: 257 4596 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 646 678 646 Size: 32 4821 AlwNI CAG_nnn'CTG Cuts at: 561 909 933 1107 1272 1839 2158 3725 561 Size: 348 24 174 165 567 319 1567 1689 Fragments arranged by size: 1689 1567 567 348 319 174 165 24 ApaI G_GGCC'C Cuts at: 630 630 Size: 4853 ApaLI G'TGCA_C Cuts at: 417 748 1015 3623 417 Size: 331 267 2608 1647 Fragments arranged by size: 2608 1647 331 267 ApoI r'AATT_y Cuts at: 1857 4206 4462 1857 Size: 2349 256 2248 Fragments arranged by size: 2349 2248 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4853 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4853 AvaI C'yCGr_G Cuts at: 589 1873 589 Size: 1284 3569 BamHI G'GATC_C Cuts at: 1878 1878 Size: 4853 BanI G'GyrC_C Cuts at: 279 772 1005 1591 1657 1681 1869 2283 2531 Size: 493 233 586 66 24 188 414 248 Cuts at: 2531 2566 279 Size: 35 2566 Fragments arranged by size: 2566 586 493 414 248 233 188 66 35 24 BanII G_rGCy'C Cuts at: 300 630 698 848 1262 1438 1829 1867 2897 Size: 330 68 150 414 176 391 38 1030 Cuts at: 2897 300 Size: 2256 Fragments arranged by size: 2256 1030 414 391 330 176 150 68 38 BbeI G_GCGC'C Cuts at: 283 1595 1661 2287 2535 283 Size: 1312 66 626 248 2601 Fragments arranged by size: 2601 1312 626 248 66 BciVI GTATCCnnnnn_n' Cuts at: 2751 3512 4853 2751 Size: 761 1341 2751 Fragments arranged by size: 2751 1341 761 BclI T'GATC_A Cuts at: 2373 2373 Size: 4853 BglI GCCn_nnn'nGGC Cuts at: 511 739 1345 1507 1655 1780 511 Size: 228 606 162 148 125 3584 Fragments arranged by size: 3584 606 228 162 148 125 BglII A'GATC_T Cuts at: 395 2368 4405 395 Size: 1973 2037 843 Fragments arranged by size: 2037 1973 843 BlpI GC'TnA_GC Cuts at: 1151 1965 1151 Size: 814 4039 Bme1580I G_kGCm'C Cuts at: 421 630 752 1010 1019 1814 2478 2571 3627 Size: 209 122 258 9 795 664 93 1056 Cuts at: 3627 421 Size: 1647 Fragments arranged by size: 1647 1056 795 664 258 209 122 93 9 BmrI ACTGGGnnnn_n' Cuts at: 1029 1372 2065 2146 2481 4625 1029 Size: 343 693 81 335 2144 1257 Fragments arranged by size: 2144 1257 693 343 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3220 3220 Size: 4853 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 213 862 886 1294 1662 1768 1861 213 Size: 649 24 408 368 106 93 3205 Fragments arranged by size: 3205 649 408 368 106 93 24 Bpu10I CC'TnA_GC Cuts at: 1516 1741 1801 1516 Size: 225 60 4568 Fragments arranged by size: 4568 225 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 616 688 1708 2029 2908 3400 3698 616 Size: 72 1020 321 879 492 298 1771 Fragments arranged by size: 1771 1020 879 492 321 298 72 BsaI GGTCTCn'nnnn_ Cuts at: 380 1744 1837 4557 380 Size: 1364 93 2720 676 Fragments arranged by size: 2720 1364 676 93 BsaAI yAC'GTr Cuts at: 543 1664 2206 543 Size: 1121 542 3190 Fragments arranged by size: 3190 1121 542 BsaBI GATnn'nnATC Cuts at: 6 2367 4404 6 Size: 2361 2037 455 Fragments arranged by size: 2361 2037 455 BsaHI Gr'CG_yC Cuts at: 280 1334 1592 1658 2284 2532 3234 4730 280 Size: 1054 258 66 626 248 702 1496 403 Fragments arranged by size: 1496 1054 702 626 403 258 248 66 BsaWI w'CCGG_w Cuts at: 117 2042 2563 3241 3515 3662 4073 117 Size: 1925 521 678 274 147 411 897 Fragments arranged by size: 1925 897 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 460 490 460 Size: 30 4823 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 280 345 1291 1837 280 Size: 65 946 546 3296 Fragments arranged by size: 3296 946 546 65 BseYI C'CCAG_C Cuts at: 549 877 893 1815 2278 3036 3613 4109 549 Size: 328 16 922 463 758 577 496 1293 Fragments arranged by size: 1293 922 758 577 496 463 328 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 136 142 748 814 136 Size: 6 606 66 4175 Fragments arranged by size: 4175 606 66 6 BsiEI CG_ry'CG Cuts at: 104 118 2395 2441 3649 104 Size: 14 2277 46 1208 1308 Fragments arranged by size: 2277 1308 1208 46 14 BsiHKAI G_wGCw'C Cuts at: 260 421 568 698 752 848 1019 1046 1517 Size: 161 147 130 54 96 171 27 471 Cuts at: 1517 1791 1829 1867 2645 2835 3627 260 Size: 274 38 38 778 190 792 1486 Fragments arranged by size: 1486 792 778 471 274 190 171 161 147 130 96 54 38 38 27 Bsp1286I G_dGCh'C Cuts at: 260 300 421 568 630 698 752 848 1010 Size: 40 121 147 62 68 54 96 162 Cuts at: 1010 1019 1046 1262 1438 1517 1791 1814 1829 Size: 9 27 216 176 79 274 23 15 Cuts at: 1829 1867 2478 2571 2645 2835 2897 3627 260 Size: 38 611 93 74 190 62 730 1486 Fragments arranged by size: 1486 730 611 274 216 190 176 162 147 121 96 93 79 74 68 62 62 54 40 38 27 23 15 9 BspEI T'CCGG_A Cuts at: 4073 4073 Size: 4853 BspHI T'CATG_A Cuts at: 4029 4029 Size: 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 362 587 815 1903 2117 2419 2800 362 Size: 225 228 1088 214 302 381 2415 Fragments arranged by size: 2415 1088 381 302 228 225 214 BsrBI CCG'CTC Cuts at: 50 3145 3199 4683 4848 50 Size: 3095 54 1484 165 55 Fragments arranged by size: 3095 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2765 2765 Size: 4853 BsrFI r'CCGG_y Cuts at: 117 1063 1168 2851 3032 3241 117 Size: 946 105 1683 181 209 1729 Fragments arranged by size: 1729 1683 946 209 181 105 BssHII G'CGCG_C Cuts at: 1569 1569 Size: 4853 BssSI C'ACGA_G Cuts at: 343 691 1784 1807 3124 3482 343 Size: 348 1093 23 1317 358 1714 Fragments arranged by size: 1714 1317 1093 358 348 23 BstAPI GCAn_nnn'nTGC Cuts at: 2230 2230 Size: 4853 BstBI TT'CG_AA Cuts at: 3214 3214 Size: 4853 BstXI CCAn_nnnn'nTGG Cuts at: 1542 1542 Size: 4853 BstYI r'GATC_y Cuts at: 1 395 1878 2345 2368 2703 2949 3961 4047 Size: 394 1483 467 23 335 246 1012 86 Cuts at: 4047 4059 4405 1 Size: 12 346 449 Fragments arranged by size: 1483 1012 467 449 394 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 596 596 Size: 4853 BtgI C'CryG_G Cuts at: 266 1254 1528 1598 266 Size: 988 274 70 3521 Fragments arranged by size: 3521 988 274 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2803 2984 3147 2803 Size: 181 163 4509 Fragments arranged by size: 4509 181 163 BtsI GCAGTG_nn' Cuts at: 1046 1118 2096 1046 Size: 72 978 3803 Fragments arranged by size: 3803 978 72 DraI TTT'AAA Cuts at: 1852 1852 Size: 4853 DrdI GACnn_nn'nnGTC Cuts at: 2559 3417 2559 Size: 858 3995 EaeI y'GGCC_r Cuts at: 172 1757 2438 2612 3003 3030 4375 172 Size: 1585 681 174 391 27 1345 650 Fragments arranged by size: 1585 1345 681 650 391 174 27 EagI C'GGCC_G Cuts at: 2438 2438 Size: 4853 EarI CTCTTCn'nnn_ Cuts at: 322 440 703 1451 2876 3086 322 Size: 118 263 748 1425 210 2089 Fragments arranged by size: 2089 1425 748 263 210 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3061 3371 3517 4663 3061 Size: 310 146 1146 3251 Fragments arranged by size: 3251 1146 310 146 EcoICRI GAG'CTC Cuts at: 696 846 1827 1865 696 Size: 150 981 38 3684 Fragments arranged by size: 3684 981 150 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 177 213 400 862 873 886 931 1294 1662 Size: 36 187 462 11 13 45 363 368 Cuts at: 1662 1768 1861 2678 3110 3857 4125 177 Size: 106 93 817 432 747 268 905 Fragments arranged by size: 905 817 747 462 432 368 363 268 187 106 93 45 36 13 11 EcoNI CCTnn'n_nnAGG Cuts at: 742 925 742 Size: 183 4670 EcoO109I rG'GnC_Cy Cuts at: 210 534 626 627 681 897 906 917 1992 Size: 324 92 1 54 216 9 11 1075 Cuts at: 1992 210 Size: 3071 Fragments arranged by size: 3071 1075 324 216 92 54 11 9 1 EcoRI G'AATT_C Cuts at: 1857 1857 Size: 4853 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 657 2872 657 Size: 2215 2638 FspI TGC'GCA Cuts at: 1552 2634 1552 Size: 1082 3771 HaeII r_GCGC'y Cuts at: 283 366 969 1410 1494 1595 1661 1741 2287 Size: 83 603 441 84 101 66 80 546 Cuts at: 2287 2535 3557 4536 283 Size: 248 1022 979 600 Fragments arranged by size: 1022 979 603 600 546 441 248 101 84 83 80 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 460 492 2695 2725 2727 2757 460 Size: 32 2203 30 2 30 2556 Fragments arranged by size: 2556 2203 32 30 30 2 HincII GTy'rAC Cuts at: 1077 1892 1077 Size: 815 4038 KasI G'GCGC_C Cuts at: 279 1591 1657 2283 2531 279 Size: 1312 66 626 248 2601 Fragments arranged by size: 2601 1312 626 248 66 KpnI G_GTAC'C Cuts at: 1873 1873 Size: 4853 MfeI C'AATT_G Cuts at: 722 722 Size: 4853 MluI A'CGCG_T Cuts at: 4286 4286 Size: 4853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3524 3708 3524 Size: 184 4669 MscI TGG'CCA Cuts at: 1759 2614 1759 Size: 855 3998 MslI CAynn'nnrTG Cuts at: 243 778 1510 2842 2969 243 Size: 535 732 1332 127 2127 Fragments arranged by size: 2127 1332 732 535 127 MspA1I CmG'CkG Cuts at: 268 492 893 1305 1428 1530 1819 1964 2278 Size: 224 401 412 123 102 289 145 314 Cuts at: 2278 2638 3651 3896 268 Size: 360 1013 245 1225 Fragments arranged by size: 1225 1013 412 401 360 314 289 245 224 145 123 102 NaeI GCC'GGC Cuts at: 1065 3034 1065 Size: 1969 2884 NarI GG'CG_CC Cuts at: 280 1592 1658 2284 2532 280 Size: 1312 66 626 248 2601 Fragments arranged by size: 2601 1312 626 248 66 NgoMIV G'CCGG_C Cuts at: 1063 3032 1063 Size: 1969 2884 NspI r_CATG'y Cuts at: 1906 2406 3313 1906 Size: 500 907 3446 Fragments arranged by size: 3446 907 500 PciI A'CATG_T Cuts at: 3309 3309 Size: 4853 PflMI CCAn_nnn'nTGG Cuts at: 651 840 933 1107 1195 1272 1839 651 Size: 189 93 174 88 77 567 3665 Fragments arranged by size: 3665 567 189 174 93 88 77 PfoI T'CCnGG_A Cuts at: 644 701 902 1271 644 Size: 57 201 369 4226 Fragments arranged by size: 4226 369 201 57 PmeI GTTT'AAAC Cuts at: 1852 1852 Size: 4853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 3992 Size: 32 4821 PpuMI rG'GwC_Cy Cuts at: 897 906 917 897 Size: 9 11 4833 Fragments arranged by size: 4833 11 9 PspOMI G'GGCC_C Cuts at: 626 626 Size: 4853 PstI C_TGCA'G Cuts at: 395 1900 2585 395 Size: 1505 685 2663 Fragments arranged by size: 2663 1505 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4853 PvuII CAG'CTG Cuts at: 893 1305 1428 2278 2638 893 Size: 412 123 850 360 3108 Fragments arranged by size: 3108 850 412 360 123 RsrII CG'GwC_CG Cuts at: 3048 3048 Size: 4853 SacI G_AGCT'C Cuts at: 698 848 1829 1867 698 Size: 150 981 38 3684 Fragments arranged by size: 3684 981 150 38 SacII CC_GC'GG Cuts at: 269 1531 269 Size: 1262 3591 SalI G'TCGA_C Cuts at: 1890 1890 Size: 4853 SanDI GG'GwC_CC Cuts at: 917 917 Size: 4853 SapI GCTCTTCn'nnn_ Cuts at: 703 2876 3086 703 Size: 2173 210 2470 Fragments arranged by size: 2470 2173 210 SbfI CC_TGCA'GG Cuts at: 1900 1900 Size: 4853 ScaI AGT'ACT Cuts at: 4370 4370 Size: 4853 SfcI C'TryA_G Cuts at: 33 391 1896 2581 3574 3765 33 Size: 358 1505 685 993 191 1121 Fragments arranged by size: 1505 1121 993 685 358 191 SfoI GGC'GCC Cuts at: 281 1593 1659 2285 2533 281 Size: 1312 66 626 248 2601 Fragments arranged by size: 2601 1312 626 248 66 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4853 SmaI CCC'GGG Cuts at: 1875 1875 Size: 4853 SmlI C'TyrA_G Cuts at: 631 667 1723 2008 2923 3415 3677 631 Size: 36 1056 285 915 492 262 1807 Fragments arranged by size: 1807 1056 915 492 285 262 36 SpeI A'CTAG_T Cuts at: 67 67 Size: 4853 SphI G_CATG'C Cuts at: 1906 2406 1906 Size: 500 4353 StuI AGG'CCT Cuts at: 1144 1144 Size: 4853 StyI C'CwwG_G Cuts at: 220 1703 1754 1987 2341 220 Size: 1483 51 233 354 2732 Fragments arranged by size: 2732 1483 354 233 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2436 2569 3028 3237 2436 Size: 133 459 209 4052 Fragments arranged by size: 4052 459 209 133 TatI w'GTAC_w Cuts at: 797 4368 797 Size: 3571 1282 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 397 429 4370 4402 397 Size: 32 3941 32 848 Fragments arranged by size: 3941 848 32 32 Tth111I GACn'n_nGTC Cuts at: 2650 2650 Size: 4853 XbaI T'CTAG_A Cuts at: 1884 1884 Size: 4853 XcmI CCAnnnn_n'nnnnTGG Cuts at: 270 2348 270 Size: 2078 2775 XmaI C'CCGG_G Cuts at: 1873 1873 Size: 4853 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AhdI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI