(Linear) (Six-base) MAPSORT of: KIEE0981.seq Check: 4792 from: 1 to: 1740 KIEE0981.orf With 182 enzymes: SgfI * April 24, 2009 13:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 479 1740 Size: 479 1261 AccI GT'mk_AC Cuts at: 0 487 1568 1740 Size: 487 1081 172 Fragments arranged by size: 1081 487 172 AclI AA'CG_TT Cuts at: 0 1094 1740 Size: 1094 646 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 292 765 823 1740 Size: 69 223 473 58 917 Fragments arranged by size: 917 473 223 69 58 AfeI AGC'GCT Cuts at: 0 859 1384 1740 Size: 859 525 356 Fragments arranged by size: 859 525 356 AgeI A'CCGG_T Cuts at: 0 9 1740 Size: 9 1731 AleI CACnn'nnGTG Cuts at: 0 135 670 1740 Size: 135 535 1070 Fragments arranged by size: 1070 535 135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 949 1206 1740 Size: 949 257 534 Fragments arranged by size: 949 534 257 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 538 570 1740 Size: 538 32 1170 Fragments arranged by size: 1170 538 32 AlwNI CAG_nnn'CTG Cuts at: 0 453 801 825 999 1164 1731 1740 Size: 453 348 24 174 165 567 9 Fragments arranged by size: 567 453 348 174 165 24 9 ApaI G_GGCC'C Cuts at: 0 522 1740 Size: 522 1218 ApaLI G'TGCA_C Cuts at: 0 309 640 907 1740 Size: 309 331 267 833 Fragments arranged by size: 833 331 309 267 AvaI C'yCGr_G Cuts at: 0 481 1740 Size: 481 1259 BanI G'GyrC_C Cuts at: 0 171 664 897 1483 1549 1573 1740 Size: 171 493 233 586 66 24 167 Fragments arranged by size: 586 493 233 171 167 66 24 BanII G_rGCy'C Cuts at: 0 192 522 590 740 1154 1330 1721 1740 Size: 192 330 68 150 414 176 391 19 Fragments arranged by size: 414 391 330 192 176 150 68 19 BbeI G_GCGC'C Cuts at: 0 175 1487 1553 1740 Size: 175 1312 66 187 Fragments arranged by size: 1312 187 175 66 BglI GCCn_nnn'nGGC Cuts at: 0 403 631 1237 1399 1547 1672 1740 Size: 403 228 606 162 148 125 68 Fragments arranged by size: 606 403 228 162 148 125 68 BglII A'GATC_T Cuts at: 0 287 1740 Size: 287 1453 BlpI GC'TnA_GC Cuts at: 0 1043 1740 Size: 1043 697 Bme1580I G_kGCm'C Cuts at: 0 313 522 644 902 911 1706 1740 Size: 313 209 122 258 9 795 34 Fragments arranged by size: 795 313 258 209 122 34 9 BmrI ACTGGGnnnn_n' Cuts at: 0 921 1264 1740 Size: 921 343 476 Fragments arranged by size: 921 476 343 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 105 754 778 1186 1554 1660 1740 Size: 105 649 24 408 368 106 80 Fragments arranged by size: 649 408 368 106 105 80 24 Bpu10I CC'TnA_GC Cuts at: 0 1408 1633 1693 1740 Size: 1408 225 60 47 Fragments arranged by size: 1408 225 60 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 580 1600 1740 Size: 508 72 1020 140 Fragments arranged by size: 1020 508 140 72 BsaI GGTCTCn'nnnn_ Cuts at: 0 272 1636 1729 1740 Size: 272 1364 93 11 Fragments arranged by size: 1364 272 93 11 BsaAI yAC'GTr Cuts at: 0 435 1556 1740 Size: 435 1121 184 Fragments arranged by size: 1121 435 184 BsaHI Gr'CG_yC Cuts at: 0 172 1226 1484 1550 1740 Size: 172 1054 258 66 190 Fragments arranged by size: 1054 258 190 172 66 BsaWI w'CCGG_w Cuts at: 0 9 1740 Size: 9 1731 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 352 382 1740 Size: 352 30 1358 Fragments arranged by size: 1358 352 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 172 237 1183 1729 1740 Size: 172 65 946 546 11 Fragments arranged by size: 946 546 172 65 11 BseYI C'CCAG_C Cuts at: 0 441 769 785 1707 1740 Size: 441 328 16 922 33 Fragments arranged by size: 922 441 328 33 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 34 640 706 1740 Size: 28 6 606 66 1034 Fragments arranged by size: 1034 606 66 28 6 BsiEI CG_ry'CG Cuts at: 0 10 1740 Size: 10 1730 BsiHKAI G_wGCw'C Cuts at: 0 152 313 460 590 644 740 911 938 Size: 152 161 147 130 54 96 171 27 Cuts at: 938 1409 1683 1721 1740 Size: 471 274 38 19 Fragments arranged by size: 471 274 171 161 152 147 130 96 54 38 27 19 Bsp1286I G_dGCh'C Cuts at: 0 152 192 313 460 522 590 644 740 Size: 152 40 121 147 62 68 54 96 Cuts at: 740 902 911 938 1154 1330 1409 1683 1706 Size: 162 9 27 216 176 79 274 23 Cuts at: 1706 1721 1740 Size: 15 19 Fragments arranged by size: 274 216 176 162 152 147 121 96 79 68 62 54 40 27 23 19 15 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 254 479 707 1740 Size: 254 225 228 1033 Fragments arranged by size: 1033 254 228 225 BsrFI r'CCGG_y Cuts at: 0 9 955 1060 1740 Size: 9 946 105 680 Fragments arranged by size: 946 680 105 9 BssHII G'CGCG_C Cuts at: 0 1461 1740 Size: 1461 279 BssSI C'ACGA_G Cuts at: 0 235 583 1676 1699 1740 Size: 235 348 1093 23 41 Fragments arranged by size: 1093 348 235 41 23 BstXI CCAn_nnnn'nTGG Cuts at: 0 1434 1740 Size: 1434 306 BstYI r'GATC_y Cuts at: 0 287 1740 Size: 287 1453 BstZ17I GTA'TAC Cuts at: 0 488 1740 Size: 488 1252 BtgI C'CryG_G Cuts at: 0 158 1146 1420 1490 1740 Size: 158 988 274 70 250 Fragments arranged by size: 988 274 250 158 70 BtsI GCAGTG_nn' Cuts at: 0 938 1010 1740 Size: 938 72 730 Fragments arranged by size: 938 730 72 EaeI y'GGCC_r Cuts at: 0 64 1649 1740 Size: 64 1585 91 Fragments arranged by size: 1585 91 64 EarI CTCTTCn'nnn_ Cuts at: 0 214 332 595 1343 1740 Size: 214 118 263 748 397 Fragments arranged by size: 748 397 263 214 118 EcoICRI GAG'CTC Cuts at: 0 588 738 1719 1740 Size: 588 150 981 21 Fragments arranged by size: 981 588 150 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 69 105 292 754 765 778 823 1186 Size: 69 36 187 462 11 13 45 363 Cuts at: 1186 1554 1660 1740 Size: 368 106 80 Fragments arranged by size: 462 368 363 187 106 80 69 45 36 13 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 634 817 1740 Size: 634 183 923 Fragments arranged by size: 923 634 183 EcoO109I rG'GnC_Cy Cuts at: 0 102 426 518 519 573 789 798 809 Size: 102 324 92 1 54 216 9 11 Cuts at: 809 1740 Size: 931 Fragments arranged by size: 931 324 216 102 92 54 11 9 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 549 1740 Size: 549 1191 FspI TGC'GCA Cuts at: 0 1444 1740 Size: 1444 296 HaeII r_GCGC'y Cuts at: 0 175 258 861 1302 1386 1487 1553 1633 Size: 175 83 603 441 84 101 66 80 Cuts at: 1633 1740 Size: 107 Fragments arranged by size: 603 441 175 107 101 84 83 80 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 352 384 1740 Size: 352 32 1356 Fragments arranged by size: 1356 352 32 HincII GTy'rAC Cuts at: 0 969 1740 Size: 969 771 KasI G'GCGC_C Cuts at: 0 171 1483 1549 1740 Size: 171 1312 66 191 Fragments arranged by size: 1312 191 171 66 MfeI C'AATT_G Cuts at: 0 614 1740 Size: 614 1126 MscI TGG'CCA Cuts at: 0 1651 1740 Size: 1651 89 MslI CAynn'nnrTG Cuts at: 0 135 670 1402 1740 Size: 135 535 732 338 Fragments arranged by size: 732 535 338 135 MspA1I CmG'CkG Cuts at: 0 160 384 785 1197 1320 1422 1711 1740 Size: 160 224 401 412 123 102 289 29 Fragments arranged by size: 412 401 289 224 160 123 102 29 NaeI GCC'GGC Cuts at: 0 957 1740 Size: 957 783 NarI GG'CG_CC Cuts at: 0 172 1484 1550 1740 Size: 172 1312 66 190 Fragments arranged by size: 1312 190 172 66 NgoMIV G'CCGG_C Cuts at: 0 955 1740 Size: 955 785 PflMI CCAn_nnn'nTGG Cuts at: 0 543 732 825 999 1087 1164 1731 1740 Size: 543 189 93 174 88 77 567 9 Fragments arranged by size: 567 543 189 174 93 88 77 9 PfoI T'CCnGG_A Cuts at: 0 536 593 794 1163 1740 Size: 536 57 201 369 577 Fragments arranged by size: 577 536 369 201 57 PpuMI rG'GwC_Cy Cuts at: 0 789 798 809 1740 Size: 789 9 11 931 Fragments arranged by size: 931 789 11 9 PspOMI G'GGCC_C Cuts at: 0 518 1740 Size: 518 1222 PstI C_TGCA'G Cuts at: 0 287 1740 Size: 287 1453 PvuII CAG'CTG Cuts at: 0 785 1197 1320 1740 Size: 785 412 123 420 Fragments arranged by size: 785 420 412 123 SacI G_AGCT'C Cuts at: 0 590 740 1721 1740 Size: 590 150 981 19 Fragments arranged by size: 981 590 150 19 SacII CC_GC'GG Cuts at: 0 161 1423 1740 Size: 161 1262 317 Fragments arranged by size: 1262 317 161 SanDI GG'GwC_CC Cuts at: 0 809 1740 Size: 809 931 SapI GCTCTTCn'nnn_ Cuts at: 0 595 1740 Size: 595 1145 SfcI C'TryA_G Cuts at: 0 283 1740 Size: 283 1457 SfoI GGC'GCC Cuts at: 0 173 1485 1551 1740 Size: 173 1312 66 189 Fragments arranged by size: 1312 189 173 66 SmlI C'TyrA_G Cuts at: 0 523 559 1615 1740 Size: 523 36 1056 125 Fragments arranged by size: 1056 523 125 36 StuI AGG'CCT Cuts at: 0 1036 1740 Size: 1036 704 StyI C'CwwG_G Cuts at: 0 112 1595 1646 1740 Size: 112 1483 51 94 Fragments arranged by size: 1483 112 94 51 TatI w'GTAC_w Cuts at: 0 689 1740 Size: 689 1051 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 289 321 1740 Size: 289 32 1419 Fragments arranged by size: 1419 289 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 162 1740 Size: 162 1578 Enzymes that do cut: AarI AccI AclI AcuI AfeI AgeI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstXI BstYI BstZ17I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MfeI MscI MslI MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SfcI SfoI SmlI StuI StyI TatI TstI XcmI Enzymes that do not cut: AatII Acc65I AflII AflIII AhdI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MluI MmeI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI