Restriction map for pF1KE0977
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BbeI        BcgI        BciVI       BclI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MamI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         161  CGCG                 CG^CG
AccIII        768  TCCGGA               T^CCGGA
AflII         256  CTTAAG               C^TTAAG
BalI          115  TGGCCA               TGG^CCA
BamHI         882  GGATCC               G^GATCC
BanI          669  GGCACC               G^G[CT][GA]CC
BbrPI          41  CACGTG               CAC^GTG
BbsI          918  GAAGAC               GAAGAC(2/6)
BbvCI         489  CCTCAGC              CCTCAGC(-5/-2)
BfaI          424  CTAG                 C^TAG
BfrI          256  CTTAAG               C^TTAAG
BglI         1525  GCCCATGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         918  GAAGAC               GAAGAC(2/6)
BsaWI         768  TCCGGA               [AT]^CCGG[AT]
BseAI         768  TCCGGA               T^CCGGA
BspEI         768  TCCGGA               T^CCGGA
BspHI         311  TCATGA               T^CATGA
BssSI        1166  CACGAG               CACGAG(-5/-1)
BstUI         161  CGCG                 CG^CG
CfoI          894  GCGC                 GCG^C
DraIII        823  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          644  CCGTGG               C^C[GA][CT]GG
EcoRI          18  GAATTC               G^AATTC
EcoRV        1108  GATATC               GAT^ATC
EcoT14I       445  CCTTGG               C^C[AT][AT]GG
HhaI          894  GCGC                 GCG^C
HinP1I        894  GCGC                 G^CGC
HincII       1195  GTCAAC               GT[CT]^[GA]AC
HindII       1195  GTCAAC               GT[CT]^[GA]AC
MaeI          424  CTAG                 C^TAG
MluI          160  ACGCGT               A^CGCGT
MluNI         115  TGGCCA               TGG^CCA
MroI          768  TCCGGA               T^CCGGA
MscI          115  TGGCCA               TGG^CCA
MslI          477  CATAGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1514  CAGCTG               C[AC]G^C[GT]G
MvnI          161  CGCG                 CG^CG
PmaCI          41  CACGTG               CAC^GTG
PmlI           41  CACGTG               CAC^GTG
PvuII        1514  CAGCTG               CAG^CTG
RcaI          311  TCATGA               T^CATGA
SacI          131  GAGCTC               GAGCT^C
SapI          955  GAAGAGC              GCTCTTC(1/4)
SgrAI        1354  CGCCGGTG             C[GA]^CCGG[CT]G
SphI          378  GCATGC               GCATG^C
StuI         1683  AGGCCT               AGG^CCT
StyI          445  CCTTGG               C^C[AT][AT]GG
TfiI         1426  GAATC                G^A[AT]TC
Tsp45I       1062  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII         42  ACGTGT               A^C[GA][CT]GT
              160  ACGCGT              
ApaI         1300  GGGCCC               GGGCC^C
             1441  GGGCCC              
AspHI         131  GAGCTC               G[AT]GC[AT]^C
             1208  GTGCTC              
BsaAI          41  CACGTG               [CT]AC^GT[GA]
             1130  CACGTA              
BseRI          80  GAGGAG               GAGGAG(10/8)
             1618  GAGGAG              
BsiHKAI       131  GAGCTC               G[AT]GC[AT]^C
             1208  GTGCTC              
BspMI         434  ACCTGC               ACCTGC(4/8)
             1250  ACCTGC              
BsrFI        1160  ACCGGC               [GA]^CCGG[CT]
             1355  GCCGGT              
Cfr10I       1160  ACCGGC               [GA]^CCGG[CT]
             1355  GCCGGT              
HgaI          159  GACGC                GACGC(5/10)
               51  GCGTC               
MfeI           23  CAATTG               C^AATTG
              373  CAATTG              
MseI          257  TTAA                 T^TAA
             1478  TTAA                
MunI           23  CAATTG               C^AATTG
              373  CAATTG              
NspI          290  ACATGC               [GA]CATG^[CT]
              378  GCATGC              
PflMI         819  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1165  CCACGAGATGG         
PleI          320  GACTC                GAGTC(4/5)
              998  GACTC               
PpuMI         524  GGGACCT              [GA]G^G[AT]CC[CT]
             1217  AGGACCT             
PstI          575  CTGCAG               CTGCA^G
              979  CTGCAG              
TaqI          129  TCGA                 T^CGA
             1095  TCGA                
Tru9I         257  TTAA                 T^TAA
             1478  TTAA                
TthHB8I       129  TCGA                 T^CGA
             1095  TCGA                
Van91I        819  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1165  CCACGAGATGG         
XcmI          992  CCAGGGGACTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1554  CCAGGAAGAAAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        AvaII       BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TseI        Tsp509I     TspRI       XhoII       
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