Restriction map for pF1KE0977
 (Linear) (Six-base) MAPSORT of: KIEE0977.seq  Check: 6716  from: 1  to: 1713



KIEE0977.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230    1713 
   Size:        230    1483
 
AflII C'TTAA_G

Cuts at:      0     256    1713 
   Size:        256    1457
 
AflIII A'CryG_T

Cuts at:      0      42     160    1713 
   Size:         42     118    1553

  Fragments arranged by size: 

                1553     118      42
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1702    1713 
   Size:       1702      11
 
ApaI G_GGCC'C

Cuts at:      0    1304    1445    1713 
   Size:       1304     141     268

  Fragments arranged by size: 

                1304     268     141
 
ApoI r'AATT_y

Cuts at:      0      18     792     932    1713 
   Size:         18     774     140     781

  Fragments arranged by size: 

                 781     774     140      18
 
BamHI G'GATC_C

Cuts at:      0     882    1713 
   Size:        882     831
 
BanI G'GyrC_C

Cuts at:      0     669    1713 
   Size:        669    1044
 
BanII G_rGCy'C

Cuts at:      0     135     695    1304    1337    1445    1713 
   Size:        135     560     609      33     108     268

  Fragments arranged by size: 

                 609     560     268     135     108      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     925    1713 
   Size:        925     788
 
BbvCI CC'TCA_GC

Cuts at:      0     490    1713 
   Size:        490    1223
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1531    1713 
   Size:       1531     182
 
Bme1580I G_kGCm'C

Cuts at:      0    1304    1445    1713 
   Size:       1304     141     268

  Fragments arranged by size: 

                1304     268     141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     946    1537    1640    1713 
   Size:        946     591     103      73

  Fragments arranged by size: 

                 946     591     103      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     490    1713 
   Size:        490    1223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1450    1505    1713 
   Size:       1450      55     208

  Fragments arranged by size: 

                1450     208      55
 
BsaAI yAC'GTr

Cuts at:      0      43    1132    1713 
   Size:         43    1089     581

  Fragments arranged by size: 

                1089     581      43
 
BsaWI w'CCGG_w

Cuts at:      0     768    1713 
   Size:        768     945
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      95    1633    1713 
   Size:         95    1538      80

  Fragments arranged by size: 

                1538      95      80
 
BseYI C'CCAG_C

Cuts at:      0    1255    1713 
   Size:       1255     458
 
BsiHKAI G_wGCw'C

Cuts at:      0     135    1212    1713 
   Size:        135    1077     501

  Fragments arranged by size: 

                1077     501     135
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     695    1212    1304    1337    1445    1713 
   Size:        135     560     517      92      33     108     268

  Fragments arranged by size: 

                 560     517     268     135     108      92      33
 
BspEI T'CCGG_A

Cuts at:      0     768    1713 
   Size:        768     945
 
BspHI T'CATG_A

Cuts at:      0     311    1713 
   Size:        311    1402
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     443    1259    1713 
   Size:        443     816     454

  Fragments arranged by size: 

                 816     454     443
 
BsrFI r'CCGG_y

Cuts at:      0    1160    1355    1713 
   Size:       1160     195     358

  Fragments arranged by size: 

                1160     358     195
 
BssSI C'ACGA_G

Cuts at:      0    1166    1713 
   Size:       1166     547
 
BstYI r'GATC_y

Cuts at:      0     608     882    1049    1484    1713 
   Size:        608     274     167     435     229

  Fragments arranged by size: 

                 608     435     274     229     167
 
Bsu36I CC'TnA_GG

Cuts at:      0     340     674    1674    1713 
   Size:        340     334    1000      39

  Fragments arranged by size: 

                1000     340     334      39
 
BtgI C'CryG_G

Cuts at:      0     644    1713 
   Size:        644    1069
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     732    1713 
   Size:        732     981
 
BtsI GCAGTG_nn'

Cuts at:      0     488     584    1713 
   Size:        488      96    1129

  Fragments arranged by size: 

                1129     488      96
 
DraIII CAC_nnn'GTG

Cuts at:      0     828    1713 
   Size:        828     885
 
EaeI y'GGCC_r

Cuts at:      0     115     641    1162    1713 
   Size:        115     526     521     551

  Fragments arranged by size: 

                 551     526     521     115
 
EarI CTCTTCn'nnn_

Cuts at:      0      53     102     950    1713 
   Size:         53      49     848     763

  Fragments arranged by size: 

                 848     763      53      49
 
EcoICRI GAG'CTC

Cuts at:      0     133    1713 
   Size:        133    1580
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     946    1537    1640    1713 
   Size:        230     716     591     103      73

  Fragments arranged by size: 

                 716     591     230     103      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0     525    1218    1300    1442    1580    1713 
   Size:        525     693      82     142     138     133

  Fragments arranged by size: 

                 693     525     142     138     133      82
 
EcoRI G'AATT_C

Cuts at:      0      18    1713 
   Size:         18    1695
 
EcoRV GAT'ATC

Cuts at:      0    1110    1713 
   Size:       1110     603
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     517     549     703     735    1067    1099    1713 
   Size:        517      32     154      32     332      32     614

  Fragments arranged by size: 

                 614     517     332     154      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1197    1713 
   Size:       1197     516
 
MfeI C'AATT_G

Cuts at:      0      23     373    1713 
   Size:         23     350    1340

  Fragments arranged by size: 

                1340     350      23
 
MluI A'CGCG_T

Cuts at:      0     160    1713 
   Size:        160    1553
 
MscI TGG'CCA

Cuts at:      0     117    1713 
   Size:        117    1596
 
MslI CAynn'nnrTG

Cuts at:      0     481    1713 
   Size:        481    1232
 
MspA1I CmG'CkG

Cuts at:      0    1516    1713 
   Size:       1516     197
 
NspI r_CATG'y

Cuts at:      0     294     382    1713 
   Size:        294      88    1331

  Fragments arranged by size: 

                1331     294      88
 
PasI CC'CwG_GG

Cuts at:      0    1584    1695    1713 
   Size:       1584     111      18

  Fragments arranged by size: 

                1584     111      18
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     825    1171    1713 
   Size:        825     346     542

  Fragments arranged by size: 

                 825     542     346
 
PfoI T'CCnGG_A

Cuts at:      0     403    1553    1713 
   Size:        403    1150     160

  Fragments arranged by size: 

                1150     403     160
 
PmlI CAC'GTG

Cuts at:      0      43    1713 
   Size:         43    1670
 
PpuMI rG'GwC_Cy

Cuts at:      0     525    1218    1713 
   Size:        525     693     495

  Fragments arranged by size: 

                 693     525     495
 
PsiI TTA'TAA

Cuts at:      0     139    1713 
   Size:        139    1574
 
PspOMI G'GGCC_C

Cuts at:      0    1300    1441    1713 
   Size:       1300     141     272

  Fragments arranged by size: 

                1300     272     141
 
PstI C_TGCA'G

Cuts at:      0     579     983    1713 
   Size:        579     404     730

  Fragments arranged by size: 

                 730     579     404
 
PvuII CAG'CTG

Cuts at:      0    1516    1713 
   Size:       1516     197
 
SacI G_AGCT'C

Cuts at:      0     135    1713 
   Size:        135    1578
 
SapI GCTCTTCn'nnn_

Cuts at:      0     950    1713 
   Size:        950     763
 
SfcI C'TryA_G

Cuts at:      0     575     946     979    1713 
   Size:        575     371      33     734

  Fragments arranged by size: 

                 734     575     371      33
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1355    1713 
   Size:       1355     358
 
SmlI C'TyrA_G

Cuts at:      0     256    1465    1520    1713 
   Size:        256    1209      55     193

  Fragments arranged by size: 

                1209     256     193      55
 
SphI G_CATG'C

Cuts at:      0     382    1713 
   Size:        382    1331
 
StuI AGG'CCT

Cuts at:      0    1685    1713 
   Size:       1685      28
 
StyI C'CwwG_G

Cuts at:      0     445    1713 
   Size:        445    1268
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     340    1713 
   Size:        340    1373
 
TatI w'GTAC_w

Cuts at:      0     303    1469    1713 
   Size:        303    1166     244

  Fragments arranged by size: 

                1166     303     244
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     999    1561    1713 
   Size:        999     562     152

  Fragments arranged by size: 

                 999     562     152
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI     ApaI     ApoI    BamHI     BanI
    BanII     BbsI    BbvCI     BglI Bme1580I     BpmI   Bpu10I    BpuEI
    BsaAI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrFI    BssSI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
    Hin4I   HincII     MfeI     MluI     MscI     MslI   MspA1I     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI     PstI
    PvuII     SacI     SapI     SfcI    SgrAI     SmlI     SphI     StuI
     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoNI     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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