Restriction map for pF1KE0959
 (Linear) (Six-base) MAPSORT of: KIEE0959.seq  Check: 5922  from: 1  to: 1497



KIEE0959.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1364    1497 
   Size:       1364     133
 
AlwNI CAG_nnn'CTG

Cuts at:      0     109     684     787     942    1155    1218    1289    1497 
   Size:        109     575     103     155     213      63      71     208

  Fragments arranged by size: 

                 575     213     208     155     109     103      71      63
            
 
ApaI G_GGCC'C

Cuts at:      0     442     456     575     728    1143    1225    1497 
   Size:        442      14     119     153     415      82     272

  Fragments arranged by size: 

                 442     415     272     153     119      82      14
 
ApaLI G'TGCA_C

Cuts at:      0     688    1377    1497 
   Size:        688     689     120

  Fragments arranged by size: 

                 689     688     120
 
AvaI C'yCGr_G

Cuts at:      0     728     826    1427    1497 
   Size:        728      98     601      70

  Fragments arranged by size: 

                 728     601      98      70
 
AvrII C'CTAG_G

Cuts at:      0    1305    1497 
   Size:       1305     192
 
BanI G'GyrC_C

Cuts at:      0      46      67     221     604    1497 
   Size:         46      21     154     383     893

  Fragments arranged by size: 

                 893     383     154      46      21
 
BanII G_rGCy'C

Cuts at:      0     140     283     442     456     575     728     844    1143 
   Size:        140     143     159      14     119     153     116     299

Cuts at:   1143    1225    1435    1497 
   Size:         82     210      62

  Fragments arranged by size: 

                 299     210     159     153     143     140     119     116
                  82      62      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     363     977    1497 
   Size:        363     614     520

  Fragments arranged by size: 

                 614     520     363
 
BbvCI CC'TCA_GC

Cuts at:      0    1465    1497 
   Size:       1465      32
 
BglI GCCn_nnn'nGGC

Cuts at:      0     135     347    1060    1497 
   Size:        135     212     713     437

  Fragments arranged by size: 

                 713     437     212     135
 
BlpI GC'TnA_GC

Cuts at:      0     139     337    1497 
   Size:        139     198    1160

  Fragments arranged by size: 

                1160     198     139
 
Bme1580I G_kGCm'C

Cuts at:      0      49     442     456     575     692     728    1143    1225 
   Size:         49     393      14     119     117      36     415      82

Cuts at:   1225    1381    1497 
   Size:        156     116

  Fragments arranged by size: 

                 415     393     156     119     117     116      82      49
                  36      14
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      73    1299    1497 
   Size:         73    1226     198

  Fragments arranged by size: 

                1226     198      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1039    1266    1497 
   Size:        538     501     227     231

  Fragments arranged by size: 

                 538     501     231     227
 
Bpu10I CC'TnA_GC

Cuts at:      0    1126    1465    1497 
   Size:       1126     339      32

  Fragments arranged by size: 

                1126     339      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106    1497 
   Size:        106    1391
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     800    1497 
   Size:        800     697
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     194     547    1415    1453    1497 
   Size:        194     353     868      38      44

  Fragments arranged by size: 

                 868     353     194      44      38
 
BseYI C'CCAG_C

Cuts at:      0      84     574     744     771    1497 
   Size:         84     490     170      27     726

  Fragments arranged by size: 

                 726     490     170      84      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     655    1497 
   Size:          2     653     842

  Fragments arranged by size: 

                 842     653       2
 
BsiEI CG_ry'CG

Cuts at:      0     399    1049    1497 
   Size:        399     650     448

  Fragments arranged by size: 

                 650     448     399
 
BsiHKAI G_wGCw'C

Cuts at:      0     692    1357    1381    1497 
   Size:        692     665      24     116

  Fragments arranged by size: 

                 692     665     116      24
 
BsmI GAATG_Cn'

Cuts at:      0     179    1497 
   Size:        179    1318
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     140     283     442     456     575     692     728 
   Size:         49      91     143     159      14     119     117      36

Cuts at:    728     844    1143    1225    1357    1381    1435    1497 
   Size:        116     299      82     132      24      54      62

  Fragments arranged by size: 

                 299     159     143     132     119     117     116      91
                  82      62      54      49      36      24      14
 
BspHI T'CATG_A

Cuts at:      0    1010    1497 
   Size:       1010     487
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     560     647     965    1364    1497 
   Size:        560      87     318     399     133

  Fragments arranged by size: 

                 560     399     318     133      87
 
BsrFI r'CCGG_y

Cuts at:      0     299     398     976    1497 
   Size:        299      99     578     521

  Fragments arranged by size: 

                 578     521     299      99
 
BssSI C'ACGA_G

Cuts at:      0     481    1497 
   Size:        481    1016
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     228     942    1155    1497 
   Size:        228     714     213     342

  Fragments arranged by size: 

                 714     342     228     213
 
Bsu36I CC'TnA_GG

Cuts at:      0     617    1325    1497 
   Size:        617     708     172

  Fragments arranged by size: 

                 708     617     172
 
BtgI C'CryG_G

Cuts at:      0      38     351     918    1043    1084    1497 
   Size:         38     313     567     125      41     413

  Fragments arranged by size: 

                 567     413     313     125      41      38
 
BtsI GCAGTG_nn'

Cuts at:      0     971    1063    1497 
   Size:        971      92     434

  Fragments arranged by size: 

                 971     434      92
 
EaeI y'GGCC_r

Cuts at:      0      23     301     396     464    1046    1497 
   Size:         23     278      95      68     582     451

  Fragments arranged by size: 

                 582     451     278      95      68      23
 
EagI C'GGCC_G

Cuts at:      0     396    1046    1497 
   Size:        396     650     451

  Fragments arranged by size: 

                 650     451     396
 
EarI CTCTTCn'nnn_

Cuts at:      0     202    1497 
   Size:        202    1295
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      85     149    1497 
   Size:         85      64    1348

  Fragments arranged by size: 

                1348      85      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1039    1266    1497 
   Size:        538     501     227     231

  Fragments arranged by size: 

                 538     501     231     227
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     616    1147    1474    1497 
   Size:        616     531     327      23

  Fragments arranged by size: 

                 616     531     327      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0     438     439     681     724     725    1053    1122    1140 
   Size:        438       1     242      43       1     328      69      18

Cuts at:   1140    1152    1309    1497 
   Size:         12     157     188

  Fragments arranged by size: 

                 438     328     242     188     157      69      43      18
                  12       1       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136    1399    1431    1497 
   Size:        104      32    1263      32      66

  Fragments arranged by size: 

                1263     104      66      32      32
 
MscI TGG'CCA

Cuts at:      0      25    1497 
   Size:         25    1472
 
MslI CAynn'nnrTG

Cuts at:      0      31     695    1497 
   Size:         31     664     802

  Fragments arranged by size: 

                 802     664      31
 
MspA1I CmG'CkG

Cuts at:      0     228     737     859     942    1086    1117    1205    1497 
   Size:        228     509     122      83     144      31      88     292

  Fragments arranged by size: 

                 509     292     228     144     122      88      83      31
            
 
NaeI GCC'GGC

Cuts at:      0     301     400    1497 
   Size:        301      99    1097

  Fragments arranged by size: 

                1097     301      99
 
NcoI C'CATG_G

Cuts at:      0     351    1497 
   Size:        351    1146
 
NgoMIV G'CCGG_C

Cuts at:      0     299     398    1497 
   Size:        299      99    1099

  Fragments arranged by size: 

                1099     299      99
 
NspI r_CATG'y

Cuts at:      0     902    1497 
   Size:        902     595
 
PasI CC'CwG_GG

Cuts at:      0     131     283    1218    1385    1497 
   Size:        131     152     935     167     112

  Fragments arranged by size: 

                 935     167     152     131     112
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     684    1289    1497 
   Size:        684     605     208

  Fragments arranged by size: 

                 684     605     208
 
PfoI T'CCnGG_A

Cuts at:      0     797     887    1265    1497 
   Size:        797      90     378     232

  Fragments arranged by size: 

                 797     378     232      90
 
PspOMI G'GGCC_C

Cuts at:      0     438     452     571     724    1139    1221    1497 
   Size:        438      14     119     153     415      82     276

  Fragments arranged by size: 

                 438     415     276     153     119      82      14
 
PstI C_TGCA'G

Cuts at:      0     233     278     557     584     839    1258    1497 
   Size:        233      45     279      27     255     419     239

  Fragments arranged by size: 

                 419     279     255     239     233      45      27
 
PvuII CAG'CTG

Cuts at:      0     228     942    1205    1497 
   Size:        228     714     263     292

  Fragments arranged by size: 

                 714     292     263     228
 
SacII CC_GC'GG

Cuts at:      0    1087    1497 
   Size:       1087     410
 
SfcI C'TryA_G

Cuts at:      0     229     274     553     580     835    1210    1254    1497 
   Size:        229      45     279      27     255     375      44     243

  Fragments arranged by size: 

                 375     279     255     243     229      45      44      27
            
 
SmaI CCC'GGG

Cuts at:      0     730    1497 
   Size:        730     767
 
SmlI C'TyrA_G

Cuts at:      0     121    1497 
   Size:        121    1376
 
SphI G_CATG'C

Cuts at:      0     902    1497 
   Size:        902     595
 
StuI AGG'CCT

Cuts at:      0     262     526     598    1497 
   Size:        262     264      72     899

  Fragments arranged by size: 

                 899     264     262      72
 
StyI C'CwwG_G

Cuts at:      0     170     351     386     443    1305    1497 
   Size:        170     181      35      57     862     192

  Fragments arranged by size: 

                 862     192     181     170      57      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     515     736    1398    1497 
   Size:        515     221     662      99

  Fragments arranged by size: 

                 662     515     221      99
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     749     781    1497 
   Size:        749      32     716

  Fragments arranged by size: 

                 749     716      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     106     717     891    1392    1497 
   Size:        106     611     174     501     105

  Fragments arranged by size: 

                 611     501     174     106     105
 
XmaI C'CCGG_G

Cuts at:      0     728    1497 
   Size:        728     769
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI    ApaLI     AvaI    AvrII     BanI    BanII
     BbsI    BbvCI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrFI    BssSI   BstAPI   Bsu36I     BtgI
     BtsI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    Hin4I     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI   PspOMI     PstI    PvuII    SacII     SfcI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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