Restriction map for
pF1KE0919
(Circular) (Six-base) MAPSORT of: pF1KE0919.seq Check: 3457 from: 1 to: 4028
pF1KE0919 4028 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 715 729 900 715
Size: 14 171 3843
Fragments arranged by size:
3843 171 14
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1853 2285 3032 3300 1853
Size: 432 747 268 2581
Fragments arranged by size:
2581 747 432 268
AflIII A'CryG_T
Cuts at: 440 1380 2484 3461 440
Size: 940 1104 977 1007
Fragments arranged by size:
1104 1007 977 940
AgeI A'CCGG_T
Cuts at: 806 2416 806
Size: 1610 2418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 290 290
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 1333 2900 1333
Size: 1567 2461
ApaI G_GGCC'C
Cuts at: 181 326 181
Size: 145 3883
ApaLI G'TGCA_C
Cuts at: 463 2798 463
Size: 2335 1693
ApoI r'AATT_y
Cuts at: 538 1032 3381 3637 538
Size: 494 2349 256 929
Fragments arranged by size:
2349 929 494 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 173 688 1048 173
Size: 515 360 3153
Fragments arranged by size:
3153 515 360
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 351 1044 1458 1706 1741 351
Size: 693 414 248 35 2638
Fragments arranged by size:
2638 693 414 248 35
BanII G_rGCy'C
Cuts at: 181 326 688 993 1042 2072 181
Size: 145 362 305 49 1030 2137
Fragments arranged by size:
2137 1030 362 305 145 49
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 150 184 150
Size: 34 3994
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 698 830 1548 698
Size: 132 718 3178
Fragments arranged by size:
3178 718 132
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 992 1140 992
Size: 148 3880
Bme1580I G_kGCm'C
Cuts at: 181 326 467 1653 1746 2802 181
Size: 145 141 1186 93 1056 1407
Fragments arranged by size:
1407 1186 1056 145 141 93
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 654 910 439
Size: 215 256 3557
Fragments arranged by size:
3557 256 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 835 1204 2083 2575 2873 547
Size: 288 369 879 492 298 1702
Fragments arranged by size:
1702 879 492 369 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 672 931 3732 672
Size: 259 2801 968
Fragments arranged by size:
2801 968 259
BsaAI yAC'GTr
Cuts at: 443 1381 443
Size: 938 3090
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 806 1217 1738 2416 2690 2837 3248 806
Size: 411 521 678 274 147 411 1586
Fragments arranged by size:
1586 678 521 411 411 274 147
BseYI C'CCAG_C
Cuts at: 189 1453 2211 2788 3284 189
Size: 1264 758 577 496 933
Fragments arranged by size:
1264 933 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 110
Size: 4028
BsiEI CG_ry'CG
Cuts at: 104 186 1570 1616 2824 104
Size: 82 1384 46 1208 1308
Fragments arranged by size:
1384 1308 1208 82 46
BsiHKAI G_wGCw'C
Cuts at: 467 986 1042 1820 2010 2802 467
Size: 519 56 778 190 792 1693
Fragments arranged by size:
1693 792 778 519 190 56
Bsp1286I G_dGCh'C
Cuts at: 181 326 467 688 986 993 1042 1653 1746
Size: 145 141 221 298 7 49 611 93
Cuts at: 1746 1820 2010 2072 2802 181
Size: 74 190 62 730 1407
Fragments arranged by size:
1407 730 611 298 221 190 145 141
93 74 62 49 7
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 729 900 1078 1292 1594 1975 715
Size: 14 171 178 214 302 381 2768
Fragments arranged by size:
2768 381 302 214 178 171 14
BsrBI CCG'CTC
Cuts at: 50 816 2320 2374 3858 4023 50
Size: 766 1504 54 1484 165 55
Fragments arranged by size:
1504 1484 766 165 55 54
BsrDI GCAATG_nn'
Cuts at: 950 1940 950
Size: 990 3038
BsrFI r'CCGG_y
Cuts at: 806 2026 2207 2416 806
Size: 1220 181 209 2418
Fragments arranged by size:
2418 1220 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 575 2389 575
Size: 1814 2214
BstEII G'GTnAC_C
Cuts at: 915 915
Size: 4028
BstYI r'GATC_y
Cuts at: 1 567 1053 1520 1543 1878 2124 3136 3222
Size: 566 486 467 23 335 246 1012 86
Cuts at: 3222 3234 3580 1
Size: 12 346 449
Fragments arranged by size:
1012 566 486 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 143 358 512 107
Size: 36 215 154 3623
Fragments arranged by size:
3623 215 154 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 317 1978 2159 2322 317
Size: 1661 181 163 2023
Fragments arranged by size:
2023 1661 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 183 377 471 1613 1787 2178 2205 3550 183
Size: 194 94 1142 174 391 27 1345 661
Fragments arranged by size:
1345 1142 661 391 194 174 94 27
EagI C'GGCC_G
Cuts at: 183 1613 183
Size: 1430 2598
EarI CTCTTCn'nnn_
Cuts at: 577 2051 2261 577
Size: 1474 210 2344
Fragments arranged by size:
2344 1474 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 129 511 2236 2546 2692 3838 129
Size: 382 1725 310 146 1146 319
Fragments arranged by size:
1725 1146 382 319 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 654 910 1853 2285 3032 3300 439
Size: 215 256 943 432 747 268 1167
Fragments arranged by size:
1167 943 747 432 268 256 215
EcoNI CCTnn'n_nnAGG
Cuts at: 299 299
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 177 178 322 432 583 1167 177
Size: 1 144 110 151 584 3038
Fragments arranged by size:
3038 584 151 144 110 1
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 588 2047 588
Size: 1459 2569
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1870 1900 1902 1932 1870
Size: 30 2 30 3966
Fragments arranged by size:
3966 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 2699 2883 380
Size: 2319 184 1525
Fragments arranged by size:
2319 1525 184
MscI TGG'CCA
Cuts at: 379 473 1789 379
Size: 94 1316 2618
Fragments arranged by size:
2618 1316 94
MslI CAynn'nnrTG
Cuts at: 357 2017 2144 357
Size: 1660 127 2241
Fragments arranged by size:
2241 1660 127
MspA1I CmG'CkG
Cuts at: 291 1139 1453 1813 2826 3071 291
Size: 848 314 360 1013 245 1248
Fragments arranged by size:
1248 1013 848 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 358 107
Size: 251 3777
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 392 392
Size: 4028
PfoI T'CCnGG_A
Cuts at: 416 542 416
Size: 126 3902
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PpuMI rG'GwC_Cy
Cuts at: 432 583 432
Size: 151 3877
PspOMI G'GGCC_C
Cuts at: 177 322 177
Size: 145 3883
PstI C_TGCA'G
Cuts at: 321 911 1075 1760 321
Size: 590 164 685 2589
Fragments arranged by size:
2589 685 590 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SanDI GG'GwC_CC
Cuts at: 583 583
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 321 911 1075 321
Size: 590 164 3274
Fragments arranged by size:
3274 590 164
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 317 489 907 1005 1071 1756 2749 2940
Size: 284 172 418 98 66 685 993 191
Cuts at: 2940 33
Size: 1121
Fragments arranged by size:
1121 993 685 418 284 191 172 98
66
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 175 1050 175
Size: 875 3153
SmlI C'TyrA_G
Cuts at: 562 850 1183 2098 2590 2852 562
Size: 288 333 915 492 262 1738
Fragments arranged by size:
1738 915 492 333 288 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 107 358 1162 1516 107
Size: 251 804 354 2619
Fragments arranged by size:
2619 804 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1611 1744 2203 2412 1611
Size: 133 459 209 3227
Fragments arranged by size:
3227 459 209 133
TatI w'GTAC_w
Cuts at: 824 869 3543 824
Size: 45 2674 1309
Fragments arranged by size:
2674 1309 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 909 1523 909
Size: 614 3414
XmaI C'CCGG_G
Cuts at: 173 1048 173
Size: 875 3153
XmnI GAAnn'nnTTC
Cuts at: 256 256
Size: 4028
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI