Restriction map for pF1KE0910
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BseAI       
BseRI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        Eam1105I    EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AciI          307  CCGC                 CCGC(-3/-1)
AluI          568  AGCT                 AG^CT
AocI          187  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       426  AGCGCT               AGC^GCT
AvaII         623  GGTCC                G^G[AT]CC
BalI          508  TGGCCA               TGG^CCA
BamHI          58  GGATCC               G^GATCC
BanII         655  GAGCCC               G[GA]GC[CT]^C
BbsI          471  GAAGAC               GAAGAC(2/6)
BmyI          655  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         471  GAAGAC               GAAGAC(2/6)
BsaI          236  GGTCTC               GGTCTC(1/5)
BsaWI         403  TCCGGT               [AT]^CCGG[AT]
BsiEI         455  CGGCCG               CG[GA][CT]^CG
BsmAI         237  GTCTC                GTCTC(1/5)
BsmFI         624  GTCCC                GGGAC(10/14)
Bsp1286I      655  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      464  TGTACA               T^GTACA
BspMI           4  GCAGGT               ACCTGC(4/8)
BsrGI         464  TGTACA               T^GTACA
BstYI          58  GGATCC               [GA]^GATC[CT]
Bsu36I        187  CCTGAGG              CC^T[ACGT]AGG
CfoI          427  GCGC                 GCG^C
DraI          544  TTTAAA               TTT^AAA
DsaI          443  CCACGG               C^C[GA][CT]GG
EagI          455  CGGCCG               C^GGCCG
EarI          652  GAAGAG               CTCTTC(1/4)
EclXI         455  CGGCCG               C^GGCCG
Eco47III      426  AGCGCT               AGC^GCT
Eco52I        455  CGGCCG               C^GGCCG
Eco81I        187  CCTGAGG              CC^T[ACGT]AGG
EcoRV         163  GATATC               GAT^ATC
FauI          306  CCCGC                CCCGC(4/6)
HaeII         426  AGCGCT               [GA]GCGC^[CT]
HapII         404  CCGG                 C^CGG
HhaI          427  GCGC                 GCG^C
HinP1I        427  GCGC                 G^CGC
HpaII         404  CCGG                 C^CGG
Ksp632I       652  GAAGAG               CTCTTC(1/4)
MflI           58  GGATCC               [GA]^GATC[CT]
MluNI         508  TGGCCA               TGG^CCA
MscI          508  TGGCCA               TGG^CCA
MseI          545  TTAA                 T^TAA
MslI          532  CACTTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        307  CCGCTG               C[AC]G^C[GT]G
MspI          404  CCGG                 C^CGG
NheI          244  GCTAGC               G^CTAGC
PleI          479  GAGTC                GAGTC(4/5)
PshAI         474  GACAGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          634  CTGCAG               CTGCA^G
SapI          652  GAAGAGC              GCTCTTC(1/4)
ScaI           15  AGTACT               AGT^ACT
SfaNI         586  GCATC                GCATC(5/9)
SspBI         464  TGTACA               T^GTACA
TaqI          495  TCGA                 T^CGA
Tru9I         545  TTAA                 T^TAA
Tsp45I        109  GTCAC                ^GT[GC]AC
TthHB8I       495  TCGA                 T^CGA
XhoII          58  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwI           59  GATCC                GGATC(4/5)
               58  GGATC               
AlwNI         113  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              628  CAGAGGCTG           
BpmI          412  CTCCAG               CTGGAG(16/14)
              514  CTCCAG              
BsgI          102  CTGCAC               GTGCAG(16/14)
              666  CTGCAC              
Cfr13I        149  GGGCC                G^G[ACGT]CC
              623  GGTCC               
DpnII          59  GATC                 ^GATC
              564  GATC                
EaeI          455  CGGCCG               [CT]^GGCC[GA]
              508  TGGCCA              
MaeIII        109  GTCAC                ^GT[ACGT]AC
              439  GTAAC               
MboI           59  GATC                 ^GATC
              564  GATC                
NdeII          59  GATC                 ^GATC
              564  GATC                
NlaIII        132  CATG                 CATG^
              520  CATG                
Sau3AI         59  GATC                 ^GATC
              564  GATC                
Sau96I        149  GGGCC                G^G[ACGT]CC
              623  GGTCC               
SfcI           77  CTGTAG               C^T[GA][CT]AG
              634  CTGCAG              
Tsp509I       199  AATT                 ^AATT
              582  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AfaI        BbvI        BfaI        BsaJI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       DdeI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MaeI        MboII       MnlI        MvaI        
MwoI        NlaIV       RsaI        ScrFI       TfiI        
TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com