Restriction map for pF1KE0906
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
Eam1105I    EarI        Eco47III    EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MluI        
MroI        MslI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tsp45I      Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AfaI          448  GTAC                 GT^AC
AocI          383  CCTCAGG              CC^T[ACGT]AGG
AspHI         413  GAGCTC               G[AT]GC[AT]^C
AvaII         334  GGTCC                G^G[AT]CC
BalI          339  TGGCCA               TGG^CCA
BanI           21  GGTGCC               G^G[CT][GA]CC
BanII         413  GAGCTC               G[GA]GC[CT]^C
BmyI          413  GAGCTC               G[AGT]GC[ACT]^C
BpmI          184  CTGGAG               CTGGAG(16/14)
BseRI         202  GAGGAG               GAGGAG(10/8)
BsiEI         232  CGGCCG               CG[GA][CT]^CG
BsiHKAI       413  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      413  GAGCTC               G[AGT]GC[ACT]^C
BstF5I        523  CATCC                GGATG(2/0)
BstYI         149  AGATCC               [GA]^GATC[CT]
Bsu36I        383  CCTCAGG              CC^T[ACGT]AGG
CfoI          576  GCGC                 GCG^C
EagI          232  CGGCCG               C^GGCCG
EclXI         232  CGGCCG               C^GGCCG
Eco52I        232  CGGCCG               C^GGCCG
Eco81I        383  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       272  CCTTGG               C^C[AT][AT]GG
EcoT22I       520  ATGCAT               ATGCA^T
FauI          119  CCCGC                CCCGC(4/6)
FokI          523  CATCC                GGATG(9/13)
HapII         231  CCGG                 C^CGG
HhaI          576  GCGC                 GCG^C
HinP1I        576  GCGC                 G^CGC
HincII        467  GTCAAC               GT[CT]^[GA]AC
HindII        467  GTCAAC               GT[CT]^[GA]AC
HindIII       589  AAGCTT               A^AGCTT
HinfI         211  GAATC                G^A[ACGT]TC
HpaII         231  CCGG                 C^CGG
HphI          582  GGTGA                GGTGA(8/7)
MflI          149  AGATCC               [GA]^GATC[CT]
MluNI         339  TGGCCA               TGG^CCA
MscI          339  TGGCCA               TGG^CCA
MseI          251  TTAA                 T^TAA
MspI          231  CCGG                 C^CGG
NsiI          520  ATGCAT               ATGCA^T
PflMI         355  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          625  CTGCAG               CTGCA^G
PvuII         281  CAGCTG               CAG^CTG
RsaI          448  GTAC                 GT^AC
SacI          413  GAGCTC               GAGCT^C
SfiI          340  GGCCAGGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI          272  CCTTGG               C^C[AT][AT]GG
TfiI          211  GAATC                G^A[AT]TC
Tru9I         251  TTAA                 T^TAA
Tsp509I       310  AATT                 ^AATT
Van91I        355  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          351  CCAGCCAGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         149  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwI          150  GATCC                GGATC(4/5)
              369  GATCC               
AlwNI         110  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              356  CAGGAGCTG           
BglI          271  GCCTTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              341  GCCAGGCAGGC         
BsaI          315  GAGACC               GGTCTC(1/5)
              226  GGTCTC              
BsgI          128  GTGCAG               GTGCAG(16/14)
              400  GTGCAG              
BsmAI         315  GAGAC                GTCTC(1/5)
              227  GTCTC               
BsmFI         364  GGGAC                GGGAC(10/14)
               46  GTCCC               
BstAPI        280  GCAGCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              429  GCAGCCAATGC         
DpnII         150  GATC                 ^GATC
              369  GATC                
EaeI          232  CGGCCG               [CT]^GGCC[GA]
              339  TGGCCA              
MboI          150  GATC                 ^GATC
              369  GATC                
MboII          85  GAAGA                GAAGA(8/7)
              585  GAAGA               
MspA1I        281  CAGCTG               C[AC]G^C[GT]G
              532  CAGCGG              
NdeII         150  GATC                 ^GATC
              369  GATC                
NlaIII         37  CATG                 CATG^
              519  CATG                
NlaIV          21  GGTGCC               GG[ACGT]^[ACGT]CC
              379  GGAACC              
Sau3AI        150  GATC                 ^GATC
              369  GATC                
SfcI          257  CTACAG               C^T[GA][CT]AG
              625  CTGCAG              
TspRI         330  CAGTG                CA[GC]TG[ACGT][ACGT]^
              441  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BspMI       BssKI       BstNI       Cac8I       
Cfr13I      DdeI        EcoRII      Fnu4HI      HaeIII      
ItaI        MnlI        MvaI        MwoI        Sau96I      
ScrFI       SfaNI       TseI        
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