Restriction map for
pF1KE0906
(Circular) (Six-base) MAPSORT of: pF1KE0906.seq Check: 1919 from: 1 to: 3749
pF1KE0906 3749 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 495 740 495
Size: 245 3504
AccI GT'mk_AC
Cuts at: 787 787
Size: 3749
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 206 413 1574 2006 2753 3021 140
Size: 66 207 1161 432 747 268 868
Fragments arranged by size:
1161 868 747 432 268 207 66
AflIII A'CryG_T
Cuts at: 1101 2205 3182 1101
Size: 1104 977 1668
Fragments arranged by size:
1668 1104 977
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 223 469 1054 2621 223
Size: 246 585 1567 1351
Fragments arranged by size:
1567 1351 585 246
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 753 3102 3358 753
Size: 2349 256 1144
Fragments arranged by size:
2349 1144 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 129 765 1179 1427 1462 129
Size: 636 414 248 35 2416
Fragments arranged by size:
2416 636 414 248 35
BanII G_rGCy'C
Cuts at: 525 763 1793 525
Size: 238 1030 2481
Fragments arranged by size:
2481 1030 238
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3715
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BfrBI ATG'CAT
Cuts at: 630 630
Size: 3749
BglI GCCn_nnn'nGGC
Cuts at: 385 455 385
Size: 70 3679
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 1374 1467 2523 1374
Size: 93 1056 2600
Fragments arranged by size:
2600 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 3749
Bpu10I CC'TnA_GC
Cuts at: 566 566
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 417 3453 340
Size: 77 3036 636
Fragments arranged by size:
3036 636 77
BsaAI yAC'GTr
Cuts at: 1102 1102
Size: 3749
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 325
Size: 3749
BseYI C'CCAG_C
Cuts at: 469 1174 1932 2509 3005 469
Size: 705 758 577 496 1213
Fragments arranged by size:
1213 758 705 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 529 257
Size: 272 3477
BsiEI CG_ry'CG
Cuts at: 104 343 1291 1337 2545 104
Size: 239 948 46 1208 1308
Fragments arranged by size:
1308 1208 948 239 46
BsiHKAI G_wGCw'C
Cuts at: 525 763 1541 1731 2523 525
Size: 238 778 190 792 1751
Fragments arranged by size:
1751 792 778 238 190
Bsp1286I G_dGCh'C
Cuts at: 525 763 1374 1467 1541 1731 1793 2523 525
Size: 238 611 93 74 190 62 730 1751
Fragments arranged by size:
1751 730 611 238 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 495 562 740 799 1013 1315 1696 495
Size: 67 178 59 214 302 381 2548
Fragments arranged by size:
2548 381 302 214 178 67 59
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 1747 1928 2137 1747
Size: 181 209 3359
Fragments arranged by size:
3359 209 181
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 394 543 1126 394
Size: 149 583 3017
Fragments arranged by size:
3017 583 149
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 257 774 1241 1264 1599 1845 2857 2943
Size: 256 517 467 23 335 246 1012 86
Cuts at: 2943 2955 3301 1
Size: 12 346 449
Fragments arranged by size:
1012 517 467 449 346 335 256 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 492 492
Size: 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 444 992 444
Size: 548 3201
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 340 447 1334 1508 1899 1926 3271 340
Size: 107 887 174 391 27 1345 818
Fragments arranged by size:
1345 887 818 391 174 107 27
EagI C'GGCC_G
Cuts at: 340 1334 340
Size: 994 2755
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 523 761 523
Size: 238 3511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 206 313 413 1574 2006 2753 3021 140
Size: 66 107 100 1161 432 747 268 868
Fragments arranged by size:
1161 868 747 432 268 107 100 66
EcoO109I rG'GnC_Cy
Cuts at: 888 888
Size: 3749
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 1768 372
Size: 1396 2353
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 318 336 350 1591 1621 1623 1653 304
Size: 14 18 14 1241 30 2 30 2400
Fragments arranged by size:
2400 1241 30 30 18 14 14 2
HincII GTy'rAC
Cuts at: 577 788 577
Size: 211 3538
HindIII A'AGCT_T
Cuts at: 697 697
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 449 1510 449
Size: 1061 2688
MslI CAynn'nnrTG
Cuts at: 1738 1865 1738
Size: 127 3622
MspA1I CmG'CkG
Cuts at: 391 642 860 1174 1534 2547 2792 391
Size: 251 218 314 360 1013 245 1348
Fragments arranged by size:
1348 1013 360 314 251 245 218
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NsiI A_TGCA'T
Cuts at: 632 632
Size: 3749
NspI r_CATG'y
Cuts at: 802 1302 2209 802
Size: 500 907 2342
Fragments arranged by size:
2342 907 500
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PflMI CCAn_nnn'nTGG
Cuts at: 469 469
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PstI C_TGCA'G
Cuts at: 737 796 1481 737
Size: 59 685 3005
Fragments arranged by size:
3005 685 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 391 1174 1534 391
Size: 783 360 2606
Fragments arranged by size:
2606 783 360
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 525 763 525
Size: 238 3511
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 365 733 792 1477 2470 2661 33
Size: 332 368 59 685 993 191 1121
Fragments arranged by size:
1121 993 685 368 332 191 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 455 455
Size: 3749
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 380 883 1237 380
Size: 503 354 2892
Fragments arranged by size:
2892 503 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1332 1465 1924 2133 1332
Size: 133 459 209 2948
Fragments arranged by size:
2948 459 209 133
TatI w'GTAC_w
Cuts at: 3264 3264
Size: 3749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 466 1244 466
Size: 778 2971
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI