Restriction map for pF1KE0906
 (Linear) (Six-base) MAPSORT of: KIEE0906.seq  Check: 7482  from: 1  to: 636



KIEE0906.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     387     632     636 
   Size:        387     245       4

  Fragments arranged by size: 

                 387     245       4
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      98     305     636 
   Size:         32      66     207     331

  Fragments arranged by size: 

                 331     207      66      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     115     361     636 
   Size:        115     246     275

  Fragments arranged by size: 

                 275     246     115
 
BanI G'GyrC_C

Cuts at:      0      21     636 
   Size:         21     615
 
BanII G_rGCy'C

Cuts at:      0     417     636 
   Size:        417     219
 
BfrBI ATG'CAT

Cuts at:      0     522     636 
   Size:        522     114
 
BglI GCCn_nnn'nGGC

Cuts at:      0     277     347     636 
   Size:        277      70     289

  Fragments arranged by size: 

                 289     277      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     636 
   Size:        205     431
 
Bpu10I CC'TnA_GC

Cuts at:      0     458     636 
   Size:        458     178
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     232     309     636 
   Size:        232      77     327

  Fragments arranged by size: 

                 327     232      77
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217     636 
   Size:        217     419
 
BseYI C'CCAG_C

Cuts at:      0     361     636 
   Size:        361     275
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     421     636 
   Size:        149     272     215

  Fragments arranged by size: 

                 272     215     149
 
BsiEI CG_ry'CG

Cuts at:      0     235     636 
   Size:        235     401
 
BsiHKAI G_wGCw'C

Cuts at:      0     417     636 
   Size:        417     219
 
Bsp1286I G_dGCh'C

Cuts at:      0     417     636 
   Size:        417     219
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     387     454     632     636 
   Size:        387      67     178       4

  Fragments arranged by size: 

                 387     178      67       4
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     286     435     636 
   Size:        286     149     201

  Fragments arranged by size: 

                 286     201     149
 
BstYI r'GATC_y

Cuts at:      0     149     636 
   Size:        149     487
 
Bsu36I CC'TnA_GG

Cuts at:      0     384     636 
   Size:        384     252
 
BtsI GCAGTG_nn'

Cuts at:      0     336     636 
   Size:        336     300
 
EaeI y'GGCC_r

Cuts at:      0     232     339     636 
   Size:        232     107     297

  Fragments arranged by size: 

                 297     232     107
 
EagI C'GGCC_G

Cuts at:      0     232     636 
   Size:        232     404
 
EcoICRI GAG'CTC

Cuts at:      0     415     636 
   Size:        415     221
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      98     205     305     636 
   Size:         32      66     107     100     331

  Fragments arranged by size: 

                 331     107     100      66      32
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     264     636 
   Size:        264     372
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     196     210     228     242     636 
   Size:        196      14      18      14     394

  Fragments arranged by size: 

                 394     196      18      14      14
 
HincII GTy'rAC

Cuts at:      0     469     636 
   Size:        469     167
 
HindIII A'AGCT_T

Cuts at:      0     589     636 
   Size:        589      47
 
MscI TGG'CCA

Cuts at:      0     341     636 
   Size:        341     295
 
MspA1I CmG'CkG

Cuts at:      0     283     534     636 
   Size:        283     251     102

  Fragments arranged by size: 

                 283     251     102
 
NsiI A_TGCA'T

Cuts at:      0     524     636 
   Size:        524     112
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     361     636 
   Size:        361     275
 
PstI C_TGCA'G

Cuts at:      0     629     636 
   Size:        629       7
 
PvuII CAG'CTG

Cuts at:      0     283     636 
   Size:        283     353
 
SacI G_AGCT'C

Cuts at:      0     417     636 
   Size:        417     219
 
SfcI C'TryA_G

Cuts at:      0     257     625     636 
   Size:        257     368      11

  Fragments arranged by size: 

                 368     257      11
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     347     636 
   Size:        347     289
 
StyI C'CwwG_G

Cuts at:      0     272     636 
   Size:        272     364
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     358     636 
   Size:        358     278
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     BanI    BanII    BfrBI     BglI     BpmI
   Bpu10I     BsaI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI   BstAPI    BstYI   Bsu36I     BtsI     EaeI     EagI  EcoICRI
  Eco57MI     FalI    Hin4I   HincII  HindIII     MscI   MspA1I     NsiI
    PflMI     PstI    PvuII     SacI     SfcI     SfiI     StyI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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