Restriction map for pF1KE0774
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvaI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AfaI         1194  GTAC                 GT^AC
AlwNI        1016  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1083  GGGCCC               GGGCC^C
Asp700I      1099  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         717  GTGCTC               G[AT]GC[AT]^C
BalI         1285  TGGCCA               TGG^CCA
BanI           40  GGCACC               G^G[CT][GA]CC
BbsI          213  GTCTTC               GAAGAC(2/6)
BclI         1110  TGATCA               T^GATCA
BcnI           55  CCCGG                CC^[GC]GG
BpmI         1119  CTCCAG               CTGGAG(16/14)
BpuAI         213  GTCTTC               GAAGAC(2/6)
BsaAI        1195  TACGTA               [CT]AC^GT[GA]
BsaI           58  GGTCTC               GGTCTC(1/5)
BsiHKAI       717  GTGCTC               G[AT]GC[AT]^C
BspHI        1037  TCATGA               T^CATGA
BssKI          55  CCCGG                ^CC[ACGT]GG
Bst1107I      740  GTATAC               GTA^TAC
BstF5I        702  GGATG                GGATG(2/0)
BstZ17I       740  GTATAC               GTA^TAC
EaeI         1285  TGGCCA               [CT]^GGCC[GA]
EcoT22I       449  ATGCAT               ATGCA^T
FauI          222  GCGGG                CCCGC(4/6)
FbaI         1110  TGATCA               T^GATCA
FokI          702  GGATG                GGATG(9/13)
HapII          56  CCGG                 C^CGG
HgaI           87  GACGC                GACGC(5/10)
HindIII       782  AAGCTT               A^AGCTT
HpaII          56  CCGG                 C^CGG
MaeII        1196  ACGT                 A^CGT
MluNI        1285  TGGCCA               TGG^CCA
MscI         1285  TGGCCA               TGG^CCA
MslI         1150  CACTACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         81  CCGCTG               C[AC]G^C[GT]G
MspI           56  CCGG                 C^CGG
NciI           55  CCCGG                CC^[GC]GG
NsiI          449  ATGCAT               ATGCA^T
NspI          681  ACATGC               [GA]CATG^[CT]
PacI          658  TTAATTAA             TTAAT^TAA
RcaI         1037  TCATGA               T^CATGA
RsaI         1194  GTAC                 GT^AC
ScrFI          55  CCCGG                CC^[ACGT]GG
SnaBI        1195  TACGTA               TAC^GTA
TaqI          422  TCGA                 T^CGA
Tsp45I        296  GTGAC                ^GT[GC]AC
TthHB8I       422  TCGA                 T^CGA
XmnI         1099  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          740  GTATAC               GT^[AC][GT]AC
             1144  GTCTAC              
AciI           81  CCGC                 CCGC(-3/-1)
              222  GCGG                
AseI          453  ATTAAT               AT^TAAT
              661  ATTAAT              
AsnI          453  ATTAAT               AT^TAAT
              661  ATTAAT              
BanII        1083  GGGCCC               G[GA]GC[CT]^C
             1116  GGGCTC              
BfaI          359  CTAG                 C^TAG
              386  CTAG                
BsmAI         853  GAGAC                GTCTC(1/5)
               59  GTCTC               
BsmFI         678  GGGAC                GGGAC(10/14)
             1204  GGGAC               
BsrI          510  CCAGT                ACTGG(1/-1)
             1121  CCAGT               
BstXI         878  CCAAGGATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1149  CCACTACAATGG        
DdeI          347  CTGAG                C^T[ACGT]AG
              720  CTCAG               
EcoT14I       413  CCTTGG               C^C[AT][AT]GG
              878  CCAAGG              
MaeI          359  CTAG                 C^TAG
              386  CTAG                
MaeIII        296  GTGAC                ^GT[ACGT]AC
             1299  GTTAC               
PshBI         453  ATTAAT               AT^TAAT
              661  ATTAAT              
PstI          377  CTGCAG               CTGCA^G
             1208  CTGCAG              
SapI          238  GAAGAGC              GCTCTTC(1/4)
              424  GAAGAGC             
SfaNI         496  GATGC                GCATC(5/9)
              607  GATGC               
StyI          413  CCTTGG               C^C[AT][AT]GG
              878  CCAAGG              
TspRI         849  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1122  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        AvaII       
BbvI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BstYI       Cfr13I      DpnII       EarI        
Fnu4HI      HaeIII      HinfI       HphI        ItaI        
Ksp632I     MboI        MboII       MflI        MnlI        
MseI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      SfcI        TfiI        
Tru9I       TseI        Tsp509I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com