Restriction map for pF1KE0677
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FokI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MunI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacII       SalI        
Sau3AI      ScaI        SexAI       SfaNI       SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII         640  CGCG                 CG^CG
AcyI          720  GGCGCC               G[GA]^CG[CT]C
ApaI          528  GGGCCC               GGGCC^C
AvaI          142  CTCGAG               C^[CT]CG[GA]G
AviII         626  TGCGCA               TGC^GCA
BalI          617  TGGCCA               TGG^CCA
BanI          720  GGCGCC               G^G[CT][GA]CC
BbeI          720  GGCGCC               GGCGC^C
BfaI           28  CTAG                 C^TAG
BsaHI         720  GGCGCC               G[GA]^CG[CT]C
BsaWI         107  ACCGGA               [AT]^CCGG[AT]
BseRI         332  CTCCTC               GAGGAG(10/8)
BsgI          229  GTGCAG               GTGCAG(16/14)
BsoBI         142  CTCGAG               C^[CT]CG[GA]G
BspMI         231  GCAGGT               ACCTGC(4/8)
BsrFI         717  GCCGGC               [GA]^CCGG[CT]
BsrI          337  CCAGT                ACTGG(1/-1)
BstUI         640  CGCG                 CG^CG
Cfr10I        717  GCCGGC               [GA]^CCGG[CT]
FspI          626  TGCGCA               TGC^GCA
HgaI          548  GCGTC                GACGC(5/10)
Hin1I         720  GGCGCC               G[GA]^CG[CT]C
KasI          720  GGCGCC               G^GCGCC
MaeI           28  CTAG                 C^TAG
MaeII         115  ACGT                 A^CGT
MluNI         617  TGGCCA               TGG^CCA
MscI          617  TGGCCA               TGG^CCA
MspA1I        130  CAGCTG               C[AC]G^C[GT]G
MvnI          640  CGCG                 CG^CG
NaeI          717  GCCGGC               GCC^GGC
NarI          720  GGCGCC               GG^CGCC
NgoMIV        717  GCCGGC               G^CCGGC
PaeR7I        142  CTCGAG               C^TCGAG
PpuMI         429  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         130  CAGCTG               CAG^CTG
SacI          193  GAGCTC               GAGCT^C
SapI          190  GAAGAGC              GCTCTTC(1/4)
SbfI          433  CCTGCAGG             CCTGCA^GG
SfiI          609  GGCCAGCGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          720  GGCGCC               GGC^GCC
SmlI          142  CTCGAG               C^T[CT][GA]AG
Sse8387I      433  CCTGCAGG             CCTGCA^GG
StuI          497  AGGCCT               AGG^CCT
TaqI          143  TCGA                 T^CGA
Tsp45I        325  GTGAC                ^GT[GC]AC
TthHB8I       143  TCGA                 T^CGA
XcmI          305  CCAAGGAGCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          142  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AvaII         369  GGACC                G^G[AT]CC
              430  GGTCC               
BbsI          267  GAAGAC               GAAGAC(2/6)
              178  GTCTTC              
BglI          610  GCCAGCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              712  GCCAAGCCGGC         
BpmI          335  CTCCAG               CTGGAG(16/14)
              397  CTGGAG              
BpuAI         267  GAAGAC               GAAGAC(2/6)
              178  GTCTTC              
DdeI          285  CTCAG                C^T[ACGT]AG
              648  CTCAG               
DsaI          314  CCATGG               C^C[GA][CT]GG
              560  CCATGG              
HinfI         468  GACTC                G^A[ACGT]TC
              566  GACTC               
HphI          324  GGTGA                GGTGA(8/7)
              182  TCACC               
MaeIII        325  GTGAC                ^GT[ACGT]AC
              599  GTAAC               
NcoI          314  CCATGG               C^CATGG
              560  CCATGG              
PleI          468  GACTC                GAGTC(4/5)
              566  GACTC               
PstI          434  CTGCAG               CTGCA^G
              661  CTGCAG              
SfcI          434  CTGCAG               C^T[GA][CT]AG
              661  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       BanII       
BbvI        BcnI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DraII       
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        Ksp632I     
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       Sau96I      ScrFI       
StyI        TseI        TspRI       
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