Restriction map for pF1KB6343
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO109I    EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MfeI        MluI        MluNI       MscI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        TaqI        Tsp45I      Tth111I     TthHB8I     
Van91I      XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        477  TCCGGA               T^CCGGA
AocI          706  CCTAAGG              CC^T[ACGT]AGG
AspHI         233  GAGCAC               G[AT]GC[AT]^C
BanI          160  GGTGCC               G^G[CT][GA]CC
BbsI          784  GAAGAC               GAAGAC(2/6)
BbvI          303  GCAGC                GCAGC(8/12)
BfaI          230  CTAG                 C^TAG
BglII         190  AGATCT               A^GATCT
BmyI          233  GAGCAC               G[AGT]GC[ACT]^C
BpmI          371  CTCCAG               CTGGAG(16/14)
BpuAI         784  GAAGAC               GAAGAC(2/6)
BsaBI         713  GATGGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         477  TCCGGA               [AT]^CCGG[AT]
BseAI         477  TCCGGA               T^CCGGA
BseRI         320  CTCCTC               GAGGAG(10/8)
BsiHKAI       233  GAGCAC               G[AT]GC[AT]^C
BsmAI         119  GAGAC                GTCTC(1/5)
BsmFI         430  GTCCC                GGGAC(10/14)
Bsp1286I      233  GAGCAC               G[AGT]GC[ACT]^C
BspEI         477  TCCGGA               T^CCGGA
BspHI          53  TCATGA               T^CATGA
BsrI          219  CCAGT                ACTGG(1/-1)
Bsu36I        706  CCTAAGG              CC^T[ACGT]AGG
DraI          610  TTTAAA               TTT^AAA
Eco81I        706  CCTAAGG              CC^T[ACGT]AGG
EcoNI         692  CCTTTAATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         717  GATATC               GAT^ATC
EcoT14I        66  CCATGG               C^C[AT][AT]GG
Fnu4HI        303  GCAGC                GC^[ACGT]GC
HapII         478  CCGG                 C^CGG
HgaI          180  GCGTC                GACGC(5/10)
HpaII         478  CCGG                 C^CGG
ItaI          303  GCAGC                GC^[ACGT]GC
MaeI          230  CTAG                 C^TAG
MamI          713  GATGGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          477  TCCGGA               T^CCGGA
MslI           67  CATGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        425  CAGCTG               C[AC]G^C[GT]G
MspI          478  CCGG                 C^CGG
MwoI          630  GCTACTATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI           66  CCATGG               C^CATGG
NlaIV         160  GGTGCC               GG[ACGT]^[ACGT]CC
PvuII         425  CAGCTG               CAG^CTG
RcaI           53  TCATGA               T^CATGA
ScaI          437  AGTACT               AGT^ACT
SspI          727  AATATT               AAT^ATT
StyI           66  CCATGG               C^C[AT][AT]GG
TfiI          778  GAATC                G^A[AT]TC
TseI          303  GCAGC                G^C[AT]GC
XbaI          229  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AfaI          438  GTAC                 GT^AC
              772  GTAC                
BsrDI         366  CATTGC               GCAATG(2/0)
              470  GCAATG              
Cac8I          39  GCAAGC               GC[ACGT]^[ACGT]GC
              306  GCAAGC              
DsaI           66  CCATGG               C^C[GA][CT]GG
              156  CCGTGG              
MaeIII        152  GTAAC                ^GT[ACGT]AC
              284  GTAAC               
PleI          318  GACTC                GAGTC(4/5)
              702  GACTC               
RsaI          438  GTAC                 GT^AC
              772  GTAC                
SfcI          734  CTATAG               C^T[GA][CT]AG
              755  CTACAG              
TspRI         389  CACTG                CA[GC]TG[ACGT][ACGT]^
              768  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        AvaII       
BsaJI       BsiYI       BslI        BssKI       BstF5I      
BstNI       BstYI       Cfr13I      DdeI        DpnII       
EcoRII      FokI        HinfI       MboI        MboII       
MflI        MnlI        MseI        MvaI        NdeII       
NlaIII      Sau3AI      Sau96I      ScrFI       SfaNI       
Tru9I       Tsp509I     XhoII       
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