Restriction map for pF1KE0891
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaII       
AvrII       BamHI       BbrPI       BcgI        BciVI       
BclI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TaqI        
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          775  GTCTAC               GT^[AC][GT]AC
AcyI          610  GGCGCC               G[GA]^CG[CT]C
AflII         555  CTTAAG               C^TTAAG
AhdI           54  GACAACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          929  GGATC                GGATC(4/5)
AspEI          54  GACAACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          413  CCCGGG               C^[CT]CG[GA]G
AviII         424  TGCGCA               TGC^GCA
BanII         908  GAGCCC               G[GA]GC[CT]^C
BbeI          610  GGCGCC               GGCGC^C
BbvCI         689  CCTCAGC              CCTCAGC(-5/-2)
BfaI          938  CTAG                 C^TAG
BfrI          555  CTTAAG               C^TTAAG
BglI          145  GCCATCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          679  CTCCAG               CTGGAG(16/14)
BsaAI         370  TACGTG               [CT]AC^GT[GA]
BsaHI         610  GGCGCC               G[GA]^CG[CT]C
BsoBI         413  CCCGGG               C^[CT]CG[GA]G
BsrDI         768  CATTGC               GCAATG(2/0)
BsrI          155  CCAGT                ACTGG(1/-1)
BstYI         929  GGATCT               [GA]^GATC[CT]
DpnII         930  GATC                 ^GATC
DraIII        517  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          353  CCATGG               C^C[GA][CT]GG
Eam1105I       54  GACAACAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          751  CTCTTC               CTCTTC(1/4)
EcoNI         695  CCTCTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       353  CCATGG               C^C[AT][AT]GG
FspI          424  TGCGCA               TGC^GCA
HaeII         610  GGCGCC               [GA]GCGC^[CT]
HgaI          797  GACGC                GACGC(5/10)
Hin1I         610  GGCGCC               G[GA]^CG[CT]C
KasI          610  GGCGCC               G^GCGCC
Ksp632I       751  CTCTTC               CTCTTC(1/4)
MaeI          938  CTAG                 C^TAG
MboI          930  GATC                 ^GATC
MflI          929  GGATCT               [GA]^GATC[CT]
MseI          556  TTAA                 T^TAA
NarI          610  GGCGCC               GG^CGCC
NcoI          353  CCATGG               C^CATGG
NdeII         930  GATC                 ^GATC
NruI          885  TCGCGA               TCG^CGA
Sau3AI        930  GATC                 ^GATC
SfoI          610  GGCGCC               GGC^GCC
SmaI          413  CCCGGG               CCC^GGG
SmlI          555  CTTAAG               C^T[CT][GA]AG
StyI          353  CCATGG               C^C[AT][AT]GG
Tru9I         556  TTAA                 T^TAA
XhoII         929  GGATCT               [GA]^GATC[CT]
XmaCI         413  CCCGGG               C^CCGGG
XmaI          413  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         799  CGCG                 CG^CG
              886  CGCG                
AlwNI         692  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              873  CAGCCTCTG           
AspHI         567  GTGCTC               G[AT]GC[AT]^C
              676  GTGCTC              
BanI          383  GGCACC               G^G[CT][GA]CC
              610  GGCGCC              
BbsI           18  GTCTTC               GAAGAC(2/6)
              341  GTCTTC              
BpuAI          18  GTCTTC               GAAGAC(2/6)
              341  GTCTTC              
BsiHKAI       567  GTGCTC               G[AT]GC[AT]^C
              676  GTGCTC              
Bsp1407I      833  TGTACA               T^GTACA
              891  TGTACA              
BsrGI         833  TGTACA               T^GTACA
              891  TGTACA              
BssSI         402  CACGAG               CACGAG(-5/-1)
              564  CTCGTG              
BstUI         799  CGCG                 CG^CG
              886  CGCG                
CfoI          425  GCGC                 GCG^C
              611  GCGC                
DdeI          690  CTCAG                C^T[ACGT]AG
              933  CTCAG               
HhaI          425  GCGC                 GCG^C
              611  GCGC                
HinP1I        425  GCGC                 G^CGC
              611  GCGC                
MaeII         371  ACGT                 A^CGT
              461  ACGT                
MvnI          799  CGCG                 CG^CG
              886  CGCG                
SfcI          839  CTGTAG               C^T[GA][CT]AG
              870  CTACAG              
SspBI         833  TGTACA               T^GTACA
              891  TGTACA              
TfiI           29  GAATC                G^A[AT]TC
              882  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AfaI        AluI        BalI        BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
BstXI       Cac8I       Cfr13I      EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeIII      MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       PleI        PvuII       RsaI        Sau96I      
ScrFI       TseI        Tsp45I      TspRI       
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