Restriction map for pF1KE0891
 (Linear) (Six-base) MAPSORT of: KIEE0891.seq  Check: 8535  from: 1  to: 948



KIEE0891.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AccI GT'mk_AC

Cuts at:      0     776     948 
   Size:        776     172
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5     948 
   Size:          5     943
 
AflII C'TTAA_G

Cuts at:      0     555     948 
   Size:        555     393
 
AhdI GACnn_n'nnGTC

Cuts at:      0      59     948 
   Size:         59     889
 
AlwNI CAG_nnn'CTG

Cuts at:      0     697     878     948 
   Size:        697     181      70

  Fragments arranged by size: 

                 697     181      70
 
AvaI C'yCGr_G

Cuts at:      0     413     948 
   Size:        413     535
 
BanI G'GyrC_C

Cuts at:      0     383     610     948 
   Size:        383     227     338

  Fragments arranged by size: 

                 383     338     227
 
BanII G_rGCy'C

Cuts at:      0     912     948 
   Size:        912      36
 
BbeI G_GCGC'C

Cuts at:      0     614     948 
   Size:        614     334
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      11     334     948 
   Size:         11     323     614

  Fragments arranged by size: 

                 614     323      11
 
BbvCI CC'TCA_GC

Cuts at:      0     690     948 
   Size:        690     258
 
BglI GCCn_nnn'nGGC

Cuts at:      0     151     948 
   Size:        151     797
 
Bme1580I G_kGCm'C

Cuts at:      0     239     527     903     948 
   Size:        239     288     376      45

  Fragments arranged by size: 

                 376     288     239      45
 
BmgBI CAC'GTC

Cuts at:      0     462     948 
   Size:        462     486
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      48     931     948 
   Size:         48     883      17

  Fragments arranged by size: 

                 883      48      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     948 
   Size:        664     284
 
Bpu10I CC'TnA_GC

Cuts at:      0     690     948 
   Size:        690     258
 
BsaAI yAC'GTr

Cuts at:      0     372     948 
   Size:        372     576
 
BsaHI Gr'CG_yC

Cuts at:      0     611     948 
   Size:        611     337
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     451     481     948 
   Size:        451      30     467

  Fragments arranged by size: 

                 467     451      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     571     680     948 
   Size:        571     109     268

  Fragments arranged by size: 

                 571     268     109
 
Bsp1286I G_dGCh'C

Cuts at:      0     239     527     571     680     903     912     948 
   Size:        239     288      44     109     223       9      36

  Fragments arranged by size: 

                 288     239     223     109      44      36       9
 
BsrDI GCAATG_nn'

Cuts at:      0     767     948 
   Size:        767     181
 
BsrGI T'GTAC_A

Cuts at:      0     833     891     948 
   Size:        833      58      57

  Fragments arranged by size: 

                 833      58      57
 
BssSI C'ACGA_G

Cuts at:      0     402     564     948 
   Size:        402     162     384

  Fragments arranged by size: 

                 402     384     162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     120     246     258     282     948 
   Size:        120     126      12      24     666

  Fragments arranged by size: 

                 666     126     120      24      12
 
BstYI r'GATC_y

Cuts at:      0     929     948 
   Size:        929      19
 
BtgI C'CryG_G

Cuts at:      0     353     948 
   Size:        353     595
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     648     902     948 
   Size:        648     254      46

  Fragments arranged by size: 

                 648     254      46
 
BtsI GCAGTG_nn'

Cuts at:      0     623     948 
   Size:        623     325
 
DraIII CAC_nnn'GTG

Cuts at:      0     522     948 
   Size:        522     426
 
EaeI y'GGCC_r

Cuts at:      0     110     248     350     374     821     948 
   Size:        110     138     102      24     447     127

  Fragments arranged by size: 

                 447     138     127     110     102      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     757     948 
   Size:        757     191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       5     664     948 
   Size:          5     659     284

  Fragments arranged by size: 

                 659     284       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     699     948 
   Size:        699     249
 
FspI TGC'GCA

Cuts at:      0     426     948 
   Size:        426     522
 
HaeII r_GCGC'y

Cuts at:      0     614     948 
   Size:        614     334
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      45      77     948 
   Size:         45      32     871

  Fragments arranged by size: 

                 871      45      32
 
KasI G'GCGC_C

Cuts at:      0     610     948 
   Size:        610     338
 
MscI TGG'CCA

Cuts at:      0     112     250     376     823     948 
   Size:        112     138     126     447     125

  Fragments arranged by size: 

                 447     138     126     125     112
 
MslI CAynn'nnrTG

Cuts at:      0     406     829     850     948 
   Size:        406     423      21      98

  Fragments arranged by size: 

                 423     406      98      21
 
MspA1I CmG'CkG

Cuts at:      0      75     591     670     684     948 
   Size:         75     516      79      14     264

  Fragments arranged by size: 

                 516     264      79      75      14
 
NarI GG'CG_CC

Cuts at:      0     611     948 
   Size:        611     337
 
NcoI C'CATG_G

Cuts at:      0     353     948 
   Size:        353     595
 
NruI TCG'CGA

Cuts at:      0     887     948 
   Size:        887      61
 
PasI CC'CwG_GG

Cuts at:      0     321     948 
   Size:        321     627
 
PfoI T'CCnGG_A

Cuts at:      0      63     948 
   Size:         63     885
 
PvuII CAG'CTG

Cuts at:      0     591     670     684     948 
   Size:        591      79      14     264

  Fragments arranged by size: 

                 591     264      79      14
 
SfcI C'TryA_G

Cuts at:      0     839     870     948 
   Size:        839      31      78

  Fragments arranged by size: 

                 839      78      31
 
SfoI GGC'GCC

Cuts at:      0     612     948 
   Size:        612     336
 
SmaI CCC'GGG

Cuts at:      0     415     948 
   Size:        415     533
 
SmlI C'TyrA_G

Cuts at:      0     555     948 
   Size:        555     393
 
StyI C'CwwG_G

Cuts at:      0     353     948 
   Size:        353     595
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     189     592     948 
   Size:        189     403     356

  Fragments arranged by size: 

                 403     356     189
 
TatI w'GTAC_w

Cuts at:      0     179     305     833     891     948 
   Size:        179     126     528      58      57

  Fragments arranged by size: 

                 528     179     126      58      57
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     122     154     451     483     948 
   Size:        122      32     297      32     465

  Fragments arranged by size: 

                 465     297     122      32      32
 
XmaI C'CCGG_G

Cuts at:      0     413     948 
   Size:        413     535
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AhdI    AlwNI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI     BglI Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaXI  BsiHKAI Bsp1286I    BsrDI    BsrGI
    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI
     EarI  Eco57MI    EcoNI     FspI    HaeII    Hin4I     KasI     MscI
     MslI   MspA1I     NarI     NcoI     NruI     PasI     PfoI    PvuII
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmrI     BmtI    BpuEI     BsaI    BsaBI    BsaWI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NdeI   NgoMIV
     NheI     NotI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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