Restriction map for pF1KE0886
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MfeI        MluI        MroI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        TfiI        Van91I      
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AocI          900  CCTGAGG              CC^T[ACGT]AGG
AspI          838  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          602  CTCGAG               C^[CT]CG[GA]G
BfaI          328  CTAG                 C^TAG
BsaBI         621  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          796  GGTCTC               GGTCTC(1/5)
BsoBI         602  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      857  TGTACA               T^GTACA
BspHI         431  TCATGA               T^CATGA
BsrGI         857  TGTACA               T^GTACA
BsrI          150  ACTGG                ACTGG(1/-1)
BstAPI        246  GCAGAACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        867  GGTGACC              G^GT[ACGT]ACC
BstPI         867  GGTGACC              G^GT[ACGT]ACC
BstXI         110  CCACCCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         324  GGATCT               [GA]^GATC[CT]
Bsu36I        900  CCTGAGG              CC^T[ACGT]AGG
CfoI          463  GCGC                 GCG^C
Eco81I        900  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       867  GGTGACC              G^GT[ACGT]ACC
HaeII         462  GGCGCT               [GA]GCGC^[CT]
HhaI          463  GCGC                 GCG^C
HinP1I        463  GCGC                 G^CGC
HinfI         154  GACTC                G^A[ACGT]TC
MaeI          328  CTAG                 C^TAG
MamI          621  GATATTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          324  GGATCT               [GA]^GATC[CT]
MseI          608  TTAA                 T^TAA
NcoI          350  CCATGG               C^CATGG
NspI          251  ACATGC               [GA]CATG^[CT]
PaeR7I        602  CTCGAG               C^TCGAG
PflFI         838  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          154  GACTC                GAGTC(4/5)
PstI          244  CTGCAG               CTGCA^G
RcaI          431  TCATGA               T^CATGA
SfaNI          23  GCATC                GCATC(5/9)
SspBI         857  TGTACA               T^GTACA
StuI          734  AGGCCT               AGG^CCT
Tru9I         608  TTAA                 T^TAA
Tth111I       838  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          327  TCTAGA               T^CTAGA
XhoI          602  CTCGAG               C^TCGAG
XhoII         324  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          303  GTAC                 GT^AC
              858  GTAC                
AlwI          546  GATCC                GGATC(4/5)
              324  GGATC               
BbvI           66  GCAGC                GCAGC(8/12)
              243  GCTGC               
BseRI         127  CTCCTC               GAGGAG(10/8)
              693  CTCCTC              
BsgI          415  CTGCAC               GTGCAG(16/14)
              533  GTGCAG              
DpnII         325  GATC                 ^GATC
              546  GATC                
DraII         673  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              707  AGGTCCC             
DsaI          350  CCATGG               C^C[GA][CT]GG
              455  CCGTGG              
EcoO109I      673  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              707  AGGTCCC             
EcoT14I       350  CCATGG               C^C[AT][AT]GG
              669  CCTTGG              
Fnu4HI         66  GCAGC                GC^[ACGT]GC
              243  GCTGC               
HphI          618  GGTGA                GGTGA(8/7)
              867  GGTGA               
ItaI           66  GCAGC                GC^[ACGT]GC
              243  GCTGC               
MboI          325  GATC                 ^GATC
              546  GATC                
MboII         308  TCTTC                GAAGA(8/7)
              715  TCTTC               
MwoI          234  GCCCAAATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              246  GCAGAACATGC         
NdeII         325  GATC                 ^GATC
              546  GATC                
PpuMI         673  GGGTCCT              [GA]G^G[AT]CC[CT]
              707  AGGTCCC             
RsaI          303  GTAC                 GT^AC
              858  GTAC                
Sau3AI        325  GATC                 ^GATC
              546  GATC                
SfcI          244  CTGCAG               C^T[GA][CT]AG
              894  CTACAG              
SmlI          602  CTCGAG               C^T[CT][GA]AG
              703  CTCAAG              
StyI          350  CCATGG               C^C[AT][AT]GG
              669  CCTTGG              
TaqI          147  TCGA                 T^CGA
              603  TCGA                
TseI           66  GCAGC                G^C[AT]GC
              243  GCTGC               
Tsp509I       239  AATT                 ^AATT
              687  AATT                
TthHB8I       147  TCGA                 T^CGA
              603  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        AvaII       BalI        BanII       
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        EaeI        EcoRII      
FokI        HaeIII      MaeIII      MluNI       MnlI        
MscI        MslI        MvaI        NlaIII      NlaIV       
Sau96I      ScrFI       Tsp45I      TspRI       
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