Restriction map for pF1KE0886
 (Linear) (Six-base) MAPSORT of: KIEE0886.seq  Check: 6777  from: 1  to: 969



KIEE0886.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     969 
   Size:        601     368
 
AleI CACnn'nnGTG

Cuts at:      0     172     865     969 
   Size:        172     693     104

  Fragments arranged by size: 

                 693     172     104
 
AvaI C'yCGr_G

Cuts at:      0     602     969 
   Size:        602     367
 
BanII G_rGCy'C

Cuts at:      0     439     641     927     969 
   Size:        439     202     286      42

  Fragments arranged by size: 

                 439     286     202      42
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688     969 
   Size:        688     281
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     802     969 
   Size:        802     167
 
BsaBI GATnn'nnATC

Cuts at:      0     625     969 
   Size:        625     344
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     684     969 
   Size:        118     566     285

  Fragments arranged by size: 

                 566     285     118
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     554     969 
   Size:        400     154     415

  Fragments arranged by size: 

                 415     400     154
 
Bsp1286I G_dGCh'C

Cuts at:      0     439     641     927     969 
   Size:        439     202     286      42

  Fragments arranged by size: 

                 439     286     202      42
 
BspHI T'CATG_A

Cuts at:      0     431     969 
   Size:        431     538
 
BsrGI T'GTAC_A

Cuts at:      0     857     969 
   Size:        857     112
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     252     969 
   Size:        252     717
 
BstEII G'GTnAC_C

Cuts at:      0     867     969 
   Size:        867     102
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     969 
   Size:        117     852
 
BstYI r'GATC_y

Cuts at:      0     324     969 
   Size:        324     645
 
Bsu36I CC'TnA_GG

Cuts at:      0     901     969 
   Size:        901      68
 
BtgI C'CryG_G

Cuts at:      0     350     455     969 
   Size:        350     105     514

  Fragments arranged by size: 

                 514     350     105
 
EaeI y'GGCC_r

Cuts at:      0      95     347     371     515     969 
   Size:         95     252      24     144     454

  Fragments arranged by size: 

                 454     252     144      95      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     969 
   Size:        601     368
 
EcoO109I rG'GnC_Cy

Cuts at:      0     674     708     969 
   Size:        674      34     261

  Fragments arranged by size: 

                 674     261      34
 
HaeII r_GCGC'y

Cuts at:      0     466     969 
   Size:        466     503
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     969 
   Size:         81     888
 
MscI TGG'CCA

Cuts at:      0      97     349     373     517     969 
   Size:         97     252      24     144     452

  Fragments arranged by size: 

                 452     252     144      97      24
 
MslI CAynn'nnrTG

Cuts at:      0     172     844     850     865     969 
   Size:        172     672       6      15     104

  Fragments arranged by size: 

                 672     172     104      15       6
 
NcoI C'CATG_G

Cuts at:      0     350     969 
   Size:        350     619
 
NspI r_CATG'y

Cuts at:      0     255     969 
   Size:        255     714
 
PpuMI rG'GwC_Cy

Cuts at:      0     674     708     969 
   Size:        674      34     261

  Fragments arranged by size: 

                 674     261      34
 
PspXI vC'TCGA_Gb

Cuts at:      0     602     969 
   Size:        602     367
 
PstI C_TGCA'G

Cuts at:      0     248     969 
   Size:        248     721
 
SfcI C'TryA_G

Cuts at:      0     244     894     969 
   Size:        244     650      75

  Fragments arranged by size: 

                 650     244      75
 
SmlI C'TyrA_G

Cuts at:      0     602     703     969 
   Size:        602     101     266

  Fragments arranged by size: 

                 602     266     101
 
StuI AGG'CCT

Cuts at:      0     736     969 
   Size:        736     233
 
StyI C'CwwG_G

Cuts at:      0     350     669     969 
   Size:        350     319     300

  Fragments arranged by size: 

                 350     319     300
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     462     606     969 
   Size:        462     144     363

  Fragments arranged by size: 

                 462     363     144
 
TatI w'GTAC_w

Cuts at:      0     302     857     969 
   Size:        302     555     112

  Fragments arranged by size: 

                 555     302     112
 
Tth111I GACn'n_nGTC

Cuts at:      0     841     969 
   Size:        841     128
 
XbaI T'CTAG_A

Cuts at:      0     327     969 
   Size:        327     642
 
XhoI C'TCGA_G

Cuts at:      0     602     969 
   Size:        602     367
 

 Enzymes that do cut:

     AcuI     AleI     AvaI    BanII    BpuEI     BsaI    BsaBI    BseRI
     BsgI Bsp1286I    BspHI    BsrGI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     EaeI  Eco57MI EcoO109I    HaeII     MmeI     MscI
     MslI     NcoI     NspI    PpuMI    PspXI     PstI     SfcI     SmlI
     StuI     StyI    TaqII     TatI  Tth111I     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI    BstBI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI     XcmI     XmaI     XmnI     ZraI
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