Restriction map for pF1KE0854
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TfiI        Tth111I     XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          583  GTCGAC               GT^[AC][GT]AC
AcsI          743  GAATTC               [GA]^AATT[CT]
AcyI          761  GGCGCC               G[GA]^CG[CT]C
AfaI          389  GTAC                 GT^AC
AflIII        781  ACGTGT               A^C[GA][CT]GT
AlwNI         477  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          743  GAATTC               [GA]^AATT[CT]
BbeI          761  GGCGCC               GGCGC^C
BbrPI         780  CACGTG               CAC^GTG
BglI          631  GCCGATACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         780  CACGTG               [CT]AC^GT[GA]
BsaHI         761  GGCGCC               G[GA]^CG[CT]C
BseRI         169  CTCCTC               GAGGAG(10/8)
BsmAI          90  GTCTC                GTCTC(1/5)
BspHI         467  TCATGA               T^CATGA
BsrDI         767  GCAATG               GCAATG(2/0)
BstEII        654  GGTCACC              G^GT[ACGT]ACC
BstPI         654  GGTCACC              G^GT[ACGT]ACC
BstXI         299  CCAAGATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII        927  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           81  GACTTCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          638  CGGCCA               [CT]^GGCC[GA]
EarI          142  CTCTTC               CTCTTC(1/4)
EcoO65I       654  GGTCACC              G^GT[ACGT]ACC
EcoRI         743  GAATTC               G^AATTC
HgaI          618  GCGTC                GACGC(5/10)
Hin1I         761  GGCGCC               G[GA]^CG[CT]C
HincII        583  GTCGAC               GT[CT]^[GA]AC
HindII        583  GTCGAC               GT[CT]^[GA]AC
HinfI         479  GAGTC                G^A[ACGT]TC
KasI          761  GGCGCC               G^GCGCC
Ksp632I       142  CTCTTC               CTCTTC(1/4)
MaeII         781  ACGT                 A^CGT
MseI          911  TTAA                 T^TAA
MspA1I        127  CAGCTG               C[AC]G^C[GT]G
NarI          761  GGCGCC               GG^CGCC
PflMI         375  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          479  GAGTC                GAGTC(4/5)
PmaCI         780  CACGTG               CAC^GTG
PmlI          780  CACGTG               CAC^GTG
PvuII         127  CAGCTG               CAG^CTG
RcaI          467  TCATGA               T^CATGA
RsaI          389  GTAC                 GT^AC
SalI          583  GTCGAC               G^TCGAC
SfoI          761  GGCGCC               GGC^GCC
TaqI          584  TCGA                 T^CGA
Tru9I         911  TTAA                 T^TAA
Tsp509I       744  AATT                 ^AATT
TthHB8I       584  TCGA                 T^CGA
Van91I        375  CCATTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          641  CCATCAACGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI          765  CCGC                 CCGC(-3/-1)
              843  GCGG                
BanI          502  GGCACC               G^G[CT][GA]CC
              761  GGCGCC              
BanII         190  GGGCTC               G[GA]GC[CT]^C
              859  GAGCCC              
BfaI          622  CTAG                 C^TAG
              967  CTAG                
BspMI         688  ACCTGC               ACCTGC(4/8)
              839  ACCTGC              
HaeII         135  AGCGCC               [GA]GCGC^[CT]
              761  GGCGCC              
HapII         222  CCGG                 C^CGG
              555  CCGG                
HpaII         222  CCGG                 C^CGG
              555  CCGG                
MaeI          622  CTAG                 C^TAG
              967  CTAG                
MboII         143  TCTTC                GAAGA(8/7)
              593  TCTTC               
MslI           99  CATTTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              408  CACACTCATG          
MspI          222  CCGG                 C^CGG
              555  CCGG                
MwoI          129  GCTGGGAGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              631  GCCGATACGGC         
SfaNI         303  GATGC                GCATC(5/9)
               18  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AluI        BbvI        BmyI        BsaJI       BsgI        
BsiYI       BslI        BsmFI       Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HphI        ItaI        MaeIII      
MnlI        MvaI        NlaIII      NlaIV       Sau96I      
ScrFI       TseI        Tsp45I      TspRI       
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