Restriction map for pF1KE0854
 (Linear) (Six-base) MAPSORT of: KIEE0854.seq  Check: 8890  from: 1  to: 996



KIEE0854.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     697     996 
   Size:        697     299
 
AccI GT'mk_AC

Cuts at:      0     584     996 
   Size:        584     412
 
AflIII A'CryG_T

Cuts at:      0     781     996 
   Size:        781     215
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      79     996 
   Size:         79     917
 
AlwNI CAG_nnn'CTG

Cuts at:      0     482     996 
   Size:        482     514
 
ApoI r'AATT_y

Cuts at:      0     743     996 
   Size:        743     253
 
BanI G'GyrC_C

Cuts at:      0     502     761     996 
   Size:        502     259     235

  Fragments arranged by size: 

                 502     259     235
 
BanII G_rGCy'C

Cuts at:      0     194     863     996 
   Size:        194     669     133

  Fragments arranged by size: 

                 669     194     133
 
BbeI G_GCGC'C

Cuts at:      0     765     996 
   Size:        765     231
 
BglI GCCn_nnn'nGGC

Cuts at:      0     637     996 
   Size:        637     359
 
Bme1580I G_kGCm'C

Cuts at:      0     824     996 
   Size:        824     172
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     157     996 
   Size:        157     839
 
BsaAI yAC'GTr

Cuts at:      0     782     996 
   Size:        782     214
 
BsaHI Gr'CG_yC

Cuts at:      0     762     996 
   Size:        762     234
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     996 
   Size:        160     836
 
BseYI C'CCAG_C

Cuts at:      0     129     510     996 
   Size:        129     381     486

  Fragments arranged by size: 

                 486     381     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     810     996 
   Size:         97     713     186

  Fragments arranged by size: 

                 713     186      97
 
Bsp1286I G_dGCh'C

Cuts at:      0     194     824     863     996 
   Size:        194     630      39     133

  Fragments arranged by size: 

                 630     194     133      39
 
BspHI T'CATG_A

Cuts at:      0     467     996 
   Size:        467     529
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     697     848     996 
   Size:        697     151     148

  Fragments arranged by size: 

                 697     151     148
 
BsrDI GCAATG_nn'

Cuts at:      0     774     996 
   Size:        774     222
 
BstEII G'GTnAC_C

Cuts at:      0     654     996 
   Size:        654     342
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     306     996 
   Size:        306     690
 
DraIII CAC_nnn'GTG

Cuts at:      0     932     996 
   Size:        932      64
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      87     996 
   Size:         87     909
 
EaeI y'GGCC_r

Cuts at:      0     638     996 
   Size:        638     358
 
EarI CTCTTCn'nnn_

Cuts at:      0     148     996 
   Size:        148     848
 
EcoRI G'AATT_C

Cuts at:      0     743     996 
   Size:        743     253
 
HaeII r_GCGC'y

Cuts at:      0     139     765     996 
   Size:        139     626     231

  Fragments arranged by size: 

                 626     231     139
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     506     538     996 
   Size:        506      32     458

  Fragments arranged by size: 

                 506     458      32
 
HincII GTy'rAC

Cuts at:      0     585     996 
   Size:        585     411
 
KasI G'GCGC_C

Cuts at:      0     761     996 
   Size:        761     235
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     384     996 
   Size:        384     612
 
MslI CAynn'nnrTG

Cuts at:      0     103     412     996 
   Size:        103     309     584

  Fragments arranged by size: 

                 584     309     103
 
MspA1I CmG'CkG

Cuts at:      0     129     996 
   Size:        129     867
 
NarI GG'CG_CC

Cuts at:      0     762     996 
   Size:        762     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     381     996 
   Size:        381     615
 
PmlI CAC'GTG

Cuts at:      0     782     996 
   Size:        782     214
 
PvuII CAG'CTG

Cuts at:      0     129     996 
   Size:        129     867
 
SalI G'TCGA_C

Cuts at:      0     583     996 
   Size:        583     413
 
SfoI GGC'GCC

Cuts at:      0     763     996 
   Size:        763     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     134     996 
   Size:        134     862
 
TatI w'GTAC_w

Cuts at:      0     388     996 
   Size:        388     608
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     648     996 
   Size:        648     348
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AlfI    AlwNI     ApoI     BanI    BanII
     BbeI     BglI Bme1580I     BplI    BsaAI    BsaHI    BseRI    BseYI
     BsgI Bsp1286I    BspHI    BspMI    BsrDI   BstEII    BstXI   DraIII
     DrdI     EaeI     EarI    EcoRI    HaeII    Hin4I   HincII     KasI
     MmeI     MslI   MspA1I     NarI    PflMI     PmlI    PvuII     SalI
     SfoI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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