Restriction map for pF1KE0851
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       ItaI        KasI        KpnI        
Ksp632I     KspI        MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TaqI        TseI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          737  GTAC                 GT^AC
AlwI           84  GATCC                GGATC(4/5)
AocI          846  CCTGAGG              CC^T[ACGT]AGG
AseI          493  ATTAAT               AT^TAAT
AsnI          493  ATTAAT               AT^TAAT
AvaII         419  GGACC                G^G[AT]CC
BanI          597  GGTGCC               G^G[CT][GA]CC
BciVI         366  GTATCC               GTATCC(6/5)
BpmI          136  CTCCAG               CTGGAG(16/14)
BsaAI         340  TACGTG               [CT]AC^GT[GA]
BsrBI         131  CCGCTC               CCGCTC(-3/-3)
BsrDI          95  GCAATG               GCAATG(2/0)
BsrI           44  ACTGG                ACTGG(1/-1)
BssKI         416  CCTGG                ^CC[ACGT]GG
BstNI         416  CCTGG                CC^[AT]GG
Bsu36I        846  CCTGAGG              CC^T[ACGT]AGG
Cac8I         531  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr13I        419  GGACC                G^G[ACGT]CC
DpnII          84  GATC                 ^GATC
DraIII        300  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        846  CCTGAGG              CC^T[ACGT]AGG
EcoRII        416  CCTGG                ^CC[AT]GG
EcoRV         859  GATATC               GAT^ATC
HaeIII        327  GGCC                 GG^CC
HindIII       529  AAGCTT               A^AGCTT
MaeII         341  ACGT                 A^CGT
MboI           84  GATC                 ^GATC
MspA1I        400  CAGCTG               C[AC]G^C[GT]G
MvaI          416  CCTGG                CC^[AT]GG
NdeII          84  GATC                 ^GATC
NlaIV         597  GGTGCC               GG[ACGT]^[ACGT]CC
NspI          451  ACATGC               [GA]CATG^[CT]
PshBI         493  ATTAAT               AT^TAAT
PvuII         400  CAGCTG               CAG^CTG
RsaI          737  GTAC                 GT^AC
Sau3AI         84  GATC                 ^GATC
Sau96I        419  GGACC                G^G[ACGT]CC
ScrFI         416  CCTGG                CC^[ACGT]GG
SfcI          434  CTGTAG               C^T[GA][CT]AG
SmlI          526  CTCAAG               C^T[CT][GA]AG
SspI          244  AATATT               AAT^ATT
Tsp45I        509  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Asp700I         4  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              242  GAAATATTTC          
BfaI          373  CTAG                 C^TAG
              886  CTAG                
BsiYI         336  CCGCTACGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              472  CCCTTTTGCGG         
BslI          336  CCGCTACGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              472  CCCTTTTGCGG         
BsmAI         308  GTCTC                GTCTC(1/5)
              619  GTCTC               
BsmI          212  GAATGC               GAATGC(1/-1)
              395  GCATTC              
BspMI         691  ACCTGC               ACCTGC(4/8)
              733  GCAGGT              
FauI          130  CCCGC                CCCGC(4/6)
              516  CCCGC               
HphI          126  TCACC                GGTGA(8/7)
              702  TCACC               
MaeI          373  CTAG                 C^TAG
              886  CTAG                
MaeIII        193  GTAAC                ^GT[ACGT]AC
              509  GTGAC               
MseI          494  TTAA                 T^TAA
              898  TTAA                
MslI          269  CACAAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              546  CATATTTGTG          
PleI          141  GACTC                GAGTC(4/5)
              231  GACTC               
SfaNI          48  GATGC                GCATC(5/9)
              101  GCATC               
TfiI          816  GATTC                G^A[AT]TC
              828  GAATC               
Tru9I         494  TTAA                 T^TAA
              898  TTAA                
TspRI          43  CACTG                CA[GC]TG[ACGT][ACGT]^
              320  CAGTG               
XmnI            4  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              242  GAAATATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BstF5I      
DdeI        FokI        HinfI       MnlI        NlaIII      
Tsp509I     
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