Restriction map for pF1KE0813
##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssKI       BssSI       BstAPI      BstBI       
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        TaqI        Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          219  GTATAC               GT^[AC][GT]AC
AlwI          669  GATCC                GGATC(4/5)
AseI          286  ATTAAT               AT^TAAT
AsnI          286  ATTAAT               AT^TAAT
BalI          197  TGGCCA               TGG^CCA
BbsI          427  GTCTTC               GAAGAC(2/6)
BbvCI         517  GCTGAGG              CCTCAGC(-5/-2)
BfaI           70  CTAG                 C^TAG
BpuAI         427  GTCTTC               GAAGAC(2/6)
BsaAI         193  TACGTG               [CT]AC^GT[GA]
BseRI         371  CTCCTC               GAGGAG(10/8)
BsmAI         550  GTCTC                GTCTC(1/5)
Bst1107I      219  GTATAC               GTA^TAC
BstEII        567  GGTGACC              G^GT[ACGT]ACC
BstPI         567  GGTGACC              G^GT[ACGT]ACC
BstZ17I       219  GTATAC               GTA^TAC
DpnII         669  GATC                 ^GATC
DsaI          563  CCACGG               C^C[GA][CT]GG
EaeI          197  TGGCCA               [CT]^GGCC[GA]
EarI          374  CTCTTC               CTCTTC(1/4)
EcoO65I       567  GGTGACC              G^GT[ACGT]ACC
EcoRV          34  GATATC               GAT^ATC
Ksp632I       374  CTCTTC               CTCTTC(1/4)
MaeI           70  CTAG                 C^TAG
MaeII         194  ACGT                 A^CGT
MboI          669  GATC                 ^GATC
MluNI         197  TGGCCA               TGG^CCA
MscI          197  TGGCCA               TGG^CCA
MseI          287  TTAA                 T^TAA
MspA1I        515  CAGCTG               C[AC]G^C[GT]G
NdeII         669  GATC                 ^GATC
PflMI         189  CCATTACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         286  ATTAAT               AT^TAAT
PvuII         515  CAGCTG               CAG^CTG
Sau3AI        669  GATC                 ^GATC
SfcI          121  CTACAG               C^T[GA][CT]AG
SspI          340  AATATT               AAT^ATT
Tru9I         287  TTAA                 T^TAA
Tsp45I        568  GTGAC                ^GT[GC]AC
TspRI         662  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        189  CCATTACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AluI          204  AGCT                 AG^CT
              516  AGCT                
AvaII         258  GGTCC                G^G[AT]CC
              334  GGTCC               
BanII         280  GGGCTC               G[GA]GC[CT]^C
              436  GGGCTC              
BmyI          280  GGGCTC               G[AGT]GC[ACT]^C
              436  GGGCTC              
BsiYI         189  CCATTACGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              558  CCTAACCACGG         
BslI          189  CCATTACGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              558  CCTAACCACGG         
Bsp1286I      280  GGGCTC               G[AGT]GC[ACT]^C
              436  GGGCTC              
BsrI          663  ACTGG                ACTGG(1/-1)
               98  CCAGT               
BstF5I        498  CATCC                GGATG(2/0)
              711  GGATG               
FokI          498  CATCC                GGATG(9/13)
              711  GGATG               
HaeIII        198  GGCC                 GG^CC
              234  GGCC                
HinfI         348  GAATC                G^A[ACGT]TC
              506  GAATC               
HphI          567  GGTGA                GGTGA(8/7)
              666  GGTGA               
MaeIII        148  GTAAC                ^GT[ACGT]AC
              568  GTGAC               
TfiI          348  GAATC                G^A[AT]TC
              506  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AfaI        BbvI        BsaJI       BsrDI       Cfr13I      
DdeI        EcoT14I     Fnu4HI      ItaI        MboII       
MnlI        MslI        NlaIII      RsaI        Sau96I      
SfaNI       StyI        TseI        Tsp509I     
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