Restriction map for pF1KE0809
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspEI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      CpoI        
DraI        DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        Ksp632I     KspI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MseI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AlwNI         117  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         898  GTGCTC               G[AT]GC[AT]^C
AvaII         713  GGACC                G^G[AT]CC
AvrII         882  CCTAGG               C^CTAGG
BalI          167  TGGCCA               TGG^CCA
BclI          293  TGATCA               T^GATCA
BlnI          882  CCTAGG               C^CTAGG
BmyI          898  GTGCTC               G[AGT]GC[ACT]^C
BsiHKAI       898  GTGCTC               G[AT]GC[AT]^C
BsmAI         119  GTCTC                GTCTC(1/5)
Bsp1286I      898  GTGCTC               G[AGT]GC[ACT]^C
BspDI         635  ATCGAT               AT^CGAT
BspHI         347  TCATGA               T^CATGA
BspMI         741  GCAGGT               ACCTGC(4/8)
BsrI          730  ACTGG                ACTGG(1/-1)
BstBI         596  TTCGAA               TT^CGAA
BstF5I        242  GGATG                GGATG(2/0)
ClaI          635  ATCGAT               AT^CGAT
DdeI          990  CTCAG                C^T[ACGT]AG
DraII         367  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        490  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         299  CCTTTGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      367  GGGGCCT              [GA]G^G[ACGT]CC[CT]
FbaI          293  TGATCA               T^GATCA
FokI          242  GGATG                GGATG(9/13)
MluNI         167  TGGCCA               TGG^CCA
MscI          167  TGGCCA               TGG^CCA
MspA1I        266  CAGCTG               C[AC]G^C[GT]G
NcoI          620  CCATGG               C^CATGG
NspV          596  TTCGAA               TT^CGAA
PleI          648  GACTC                GAGTC(4/5)
PstI          862  CTGCAG               CTGCA^G
PvuII         266  CAGCTG               CAG^CTG
RcaI          347  TCATGA               T^CATGA
SfcI          862  CTGCAG               C^T[GA][CT]AG
SfuI          596  TTCGAA               TT^CGAA
SmlI          565  CTCAAG               C^T[CT][GA]AG
SpeI           69  ACTAGT               A^CTAGT
Tsp509I       934  AATT                 ^AATT
XcmI           99  CCACAAGAATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I        308  GGTACC               G^GTACC
              785  GGTACC              
Asp718I       308  GGTACC               G^GTACC
              785  GGTACC              
BanI          308  GGTACC               G^G[CT][GA]CC
              785  GGTACC              
BbsI          222  GAAGAC               GAAGAC(2/6)
              544  GTCTTC              
BcnI          365  CCGGG                CC^[GC]GG
              380  CCGGG               
BpuAI         222  GAAGAC               GAAGAC(2/6)
              544  GTCTTC              
BseRI         535  CTCCTC               GAGGAG(10/8)
              913  CTCCTC              
BsrDI          54  CATTGC               GCAATG(2/0)
              375  CATTGC              
DsaI          620  CCATGG               C^C[GA][CT]GG
              643  CCACGG              
EcoT14I       620  CCATGG               C^C[AT][AT]GG
              882  CCTAGG              
HapII         365  CCGG                 C^CGG
              380  CCGG                
HpaII         365  CCGG                 C^CGG
              380  CCGG                
KpnI          308  GGTACC               GGTAC^C
              785  GGTACC              
MspI          365  CCGG                 C^CGG
              380  CCGG                
NciI          365  CCGGG                CC^[GC]GG
              380  CCGGG               
NspI          560  ACATGC               [GA]CATG^[CT]
              609  ACATGC              
StyI          620  CCATGG               C^C[AT][AT]GG
              882  CCTAGG              
TaqI          597  TCGA                 T^CGA
              636  TCGA                
TspRI         460  CAGTG                CA[GC]TG[ACGT][ACGT]^
              729  CACTG               
TthHB8I       597  TCGA                 T^CGA
              636  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BfaI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BssKI       BstNI       Cac8I       Cfr13I      
DpnII       EaeI        EcoRII      Fnu4HI      HaeIII      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       TfiI        TseI        
Tsp45I      
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