Restriction map for pF1KE0795
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BamHI       BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eco47III    
Eco81I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI          891  GAATTC               [GA]^AATT[CT]
AhdI          640  GACGTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          891  GAATTC               [GA]^AATT[CT]
AspEI         640  GACGTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         745  GAGCAC               G[AT]GC[AT]^C
AvaI          277  CCCGAG               C^[CT]CG[GA]G
BanI           24  GGCGCC               G^G[CT][GA]CC
BbeI           24  GGCGCC               GGCGC^C
BbvCI         562  GCTGAGG              CCTCAGC(-5/-2)
BfaI          961  CTAG                 C^TAG
BsaWI         536  TCCGGT               [AT]^CCGG[AT]
BsiHKAI       745  GAGCAC               G[AT]GC[AT]^C
BsmBI         280  GAGACG               CGTCTC(1/5)
BsoBI         277  CCCGAG               C^[CT]CG[GA]G
BssHII        333  GCGCGC               G^CGCGC
BstXI         677  CCAAGCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         859  AGATCC               [GA]^GATC[CT]
DsaI          983  CCATGG               C^C[GA][CT]GG
Eam1105I      640  GACGTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1060  CTCTTC               CTCTTC(1/4)
EcoRI         891  GAATTC               G^AATTC
HincII        271  GTCGAC               GT[CT]^[GA]AC
HindII        271  GTCGAC               GT[CT]^[GA]AC
KasI           24  GGCGCC               G^GCGCC
Ksp632I      1060  CTCTTC               CTCTTC(1/4)
MaeI          961  CTAG                 C^TAG
MaeIII        872  GTGAC                ^GT[ACGT]AC
MflI          859  AGATCC               [GA]^GATC[CT]
MslI          748  CACCTGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI           24  GGCGCC               GG^CGCC
NcoI          983  CCATGG               C^CATGG
NruI          411  TCGCGA               TCG^CGA
PflMI         216  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          295  CTGCAG               CTGCA^G
PvuII         292  CAGCTG               CAG^CTG
SalI          271  GTCGAC               G^TCGAC
SfaNI         233  GCATC                GCATC(5/9)
SfcI          295  CTGCAG               C^T[GA][CT]AG
SfoI           24  GGCGCC               GGC^GCC
TaqI          272  TCGA                 T^CGA
Tsp45I        872  GTGAC                ^GT[GC]AC
Tsp509I       892  AATT                 ^AATT
TthHB8I       272  TCGA                 T^CGA
Van91I        216  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         859  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          271  GTCGAC               GT^[AC][GT]AC
              936  GTCTAC              
AcyI           24  GGCGCC               G[GA]^CG[CT]C
              307  GGCGTC              
AfaI          111  GTAC                 GT^AC
              464  GTAC                
BalI          980  TGGCCA               TGG^CCA
              986  TGGCCA              
BsaHI          24  GGCGCC               G[GA]^CG[CT]C
              307  GGCGTC              
BsaI          208  GAGACC               GGTCTC(1/5)
              673  GAGACC              
DdeI          563  CTGAG                C^T[ACGT]AG
              740  CTCAG               
EcoNI          48  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              816  CCTGGCAGAGG         
EcoT14I       830  CCTTGG               C^C[AT][AT]GG
              983  CCATGG              
HgaI           10  GACGC                GACGC(5/10)
              308  GCGTC               
Hin1I          24  GGCGCC               G[GA]^CG[CT]C
              307  GGCGTC              
HinfI         553  GACTC                G^A[ACGT]TC
              919  GACTC               
MaeII         197  ACGT                 A^CGT
              641  ACGT                
MluNI         980  TGGCCA               TGG^CCA
              986  TGGCCA              
MscI          980  TGGCCA               TGG^CCA
              986  TGGCCA              
MspA1I         14  CAGCGG               C[AC]G^C[GT]G
              292  CAGCTG              
NaeI          138  GCCGGC               GCC^GGC
              366  GCCGGC              
NgoMIV        138  GCCGGC               G^CCGGC
              366  GCCGGC              
PleI          553  GACTC                GAGTC(4/5)
              919  GACTC               
RsaI          111  GTAC                 GT^AC
              464  GTAC                
StyI          830  CCTTGG               C^C[AT][AT]GG
              983  CCATGG              
XcmI          677  CCAAGCTGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              842  CCAGGCCAGCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        ApaI        
AvaII       BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiEI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DpnII       DraII       EaeI        
EagI        EclXI       Eco52I      EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MboI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
TseI        TspRI       
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