Restriction map for pF1KE0795
 (Linear) (Six-base) MAPSORT of: KIEE0795.seq  Check: 9884  from: 1  to: 1074



KIEE0795.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      89     210    1074 
   Size:         89     121     864

  Fragments arranged by size: 

                 864     121      89
 
AccI GT'mk_AC

Cuts at:      0     272     937    1074 
   Size:        272     665     137

  Fragments arranged by size: 

                 665     272     137
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554    1047    1074 
   Size:        554     493      27

  Fragments arranged by size: 

                 554     493      27
 
AhdI GACnn_n'nnGTC

Cuts at:      0     645    1074 
   Size:        645     429
 
ApaI G_GGCC'C

Cuts at:      0     481     585    1002    1029    1074 
   Size:        481     104     417      27      45

  Fragments arranged by size: 

                 481     417     104      45      27
 
ApoI r'AATT_y

Cuts at:      0     891    1074 
   Size:        891     183
 
AvaI C'yCGr_G

Cuts at:      0     277    1074 
   Size:        277     797
 
BanI G'GyrC_C

Cuts at:      0      24    1074 
   Size:         24    1050
 
BanII G_rGCy'C

Cuts at:      0     131     481     578     585     841    1002    1029    1074 
   Size:        131     350      97       7     256     161      27      45

  Fragments arranged by size: 

                 350     256     161     131      97      45      27       7
            
 
BbeI G_GCGC'C

Cuts at:      0      28    1074 
   Size:         28    1046
 
BbvCI CC'TCA_GC

Cuts at:      0     563    1074 
   Size:        563     511
 
BglI GCCn_nnn'nGGC

Cuts at:      0     148     485     658    1074 
   Size:        148     337     173     416

  Fragments arranged by size: 

                 416     337     173     148
 
Bme1580I G_kGCm'C

Cuts at:      0     481     585     773    1002    1029    1074 
   Size:        481     104     188     229      27      45

  Fragments arranged by size: 

                 481     229     188     104      45      27
 
BmgBI CAC'GTC

Cuts at:      0     642    1074 
   Size:        642     432
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     476    1074 
   Size:        476     598
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     150    1074 
   Size:        150     924
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     852    1062    1074 
   Size:         70     782     210      12

  Fragments arranged by size: 

                 782     210      70      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     563    1074 
   Size:        563     511
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     202     667    1074 
   Size:        202     465     407

  Fragments arranged by size: 

                 465     407     202
 
BsaHI Gr'CG_yC

Cuts at:      0      25     308    1074 
   Size:         25     283     766

  Fragments arranged by size: 

                 766     283      25
 
BsaWI w'CCGG_w

Cuts at:      0     536    1074 
   Size:        536     538
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     538     568    1074 
   Size:        538      30     506

  Fragments arranged by size: 

                 538     506      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     337     898     933     936    1074 
   Size:        337     561      35       3     138

  Fragments arranged by size: 

                 561     337     138      35       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     403     487    1074 
   Size:         67     336      84     587

  Fragments arranged by size: 

                 587     336      84      67
 
BsiEI CG_ry'CG

Cuts at:      0      20     143     371    1074 
   Size:         20     123     228     703

  Fragments arranged by size: 

                 703     228     123      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     749    1074 
   Size:        749     325
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     274    1074 
   Size:        274     800
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     481     578     585     749     773     841    1002 
   Size:        131     350      97       7     164      24      68     161

Cuts at:   1002    1029    1074 
   Size:         27      45

  Fragments arranged by size: 

                 350     164     161     131      97      68      45      27
                  24       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      89     210     509    1074 
   Size:         89     121     299     565

  Fragments arranged by size: 

                 565     299     121      89
 
BsrFI r'CCGG_y

Cuts at:      0     138     170     366    1074 
   Size:        138      32     196     708

  Fragments arranged by size: 

                 708     196     138      32
 
BssHII G'CGCG_C

Cuts at:      0     333    1074 
   Size:        333     741
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     684    1074 
   Size:        684     390
 
BstYI r'GATC_y

Cuts at:      0     859    1074 
   Size:        859     215
 
BtgI C'CryG_G

Cuts at:      0     983    1074 
   Size:        983      91
 
EaeI y'GGCC_r

Cuts at:      0      17     140     368     980     986    1074 
   Size:         17     123     228     612       6      88

  Fragments arranged by size: 

                 612     228     123      88      17       6
 
EagI C'GGCC_G

Cuts at:      0      17     140     368    1074 
   Size:         17     123     228     706

  Fragments arranged by size: 

                 706     228     123      17
 
EarI CTCTTCn'nnn_

Cuts at:      0    1066    1074 
   Size:       1066       8
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     639     852    1074 
   Size:        639     213     222

  Fragments arranged by size: 

                 639     222     213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     554     852    1047    1062    1074 
   Size:         70     484     298     195      15      12

  Fragments arranged by size: 

                 484     298     195      70      15      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      52     820    1074 
   Size:         52     768     254

  Fragments arranged by size: 

                 768     254      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0     477     478     998    1026    1074 
   Size:        477       1     520      28      48

  Fragments arranged by size: 

                 520     477      48      28       1
 
EcoRI G'AATT_C

Cuts at:      0     891    1074 
   Size:        891     183
 
HaeII r_GCGC'y

Cuts at:      0      28     349     931    1074 
   Size:         28     321     582     143

  Fragments arranged by size: 

                 582     321     143      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     538     570     631     663     723     755    1074 
   Size:        538      32      61      32      60      32     319

  Fragments arranged by size: 

                 538     319      61      60      32      32      32
 
HincII GTy'rAC

Cuts at:      0     273    1074 
   Size:        273     801
 
KasI G'GCGC_C

Cuts at:      0      24    1074 
   Size:         24    1050
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     974    1074 
   Size:        974     100
 
MscI TGG'CCA

Cuts at:      0     982     988    1074 
   Size:        982       6      86

  Fragments arranged by size: 

                 982      86       6
 
MslI CAynn'nnrTG

Cuts at:      0     752    1074 
   Size:        752     322
 
MspA1I CmG'CkG

Cuts at:      0      16     294    1074 
   Size:         16     278     780

  Fragments arranged by size: 

                 780     278      16
 
NaeI GCC'GGC

Cuts at:      0     140     368    1074 
   Size:        140     228     706

  Fragments arranged by size: 

                 706     228     140
 
NarI GG'CG_CC

Cuts at:      0      25    1074 
   Size:         25    1049
 
NcoI C'CATG_G

Cuts at:      0     983    1074 
   Size:        983      91
 
NgoMIV G'CCGG_C

Cuts at:      0     138     366    1074 
   Size:        138     228     708

  Fragments arranged by size: 

                 708     228     138
 
NruI TCG'CGA

Cuts at:      0     413    1074 
   Size:        413     661
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     222    1074 
   Size:        222     852
 
PspOMI G'GGCC_C

Cuts at:      0     477     581     998    1025    1074 
   Size:        477     104     417      27      49

  Fragments arranged by size: 

                 477     417     104      49      27
 
PstI C_TGCA'G

Cuts at:      0     299    1074 
   Size:        299     775
 
PvuII CAG'CTG

Cuts at:      0     294    1074 
   Size:        294     780
 
SalI G'TCGA_C

Cuts at:      0     271    1074 
   Size:        271     803
 
SfcI C'TryA_G

Cuts at:      0     295    1074 
   Size:        295     779
 
SfoI GGC'GCC

Cuts at:      0      26    1074 
   Size:         26    1048
 
StyI C'CwwG_G

Cuts at:      0     830     983    1074 
   Size:        830     153      91

  Fragments arranged by size: 

                 830     153      91
 
TatI w'GTAC_w

Cuts at:      0     110    1074 
   Size:        110     964
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     684     849    1074 
   Size:        684     165     225

  Fragments arranged by size: 

                 684     225     165
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI    BbvCI     BglI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI   BssHII    BstXI
    BstYI     BtgI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI    PflMI
   PspOMI     PstI    PvuII     SalI     SfcI     SfoI     StyI     TatI
     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmtI    BpuEI    BsaAI    BsaBI    BseYI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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