Restriction map for pF1KE0760
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BbvI        
BcgI        BcnI        BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DraII       DraIII      EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
FauI        Fnu4HI      FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        ItaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        TseI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          402  GTCTAC               GT^[AC][GT]AC
AflIII        398  ACATGT               A^C[GA][CT]GT
Asp700I      1096  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1089  GAGCTC               G[AT]GC[AT]^C
BbsI          394  GAAGAC               GAAGAC(2/6)
BciVI          88  GTATCC               GTATCC(6/5)
BfaI          302  CTAG                 C^TAG
BglII         318  AGATCT               A^GATCT
BpuAI         394  GAAGAC               GAAGAC(2/6)
BsaI          337  GAGACC               GGTCTC(1/5)
BsiHKAI      1089  GAGCTC               G[AT]GC[AT]^C
BsiYI          45  CCCAGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           45  CCCAGAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         222  GGGAC                GGGAC(10/14)
BspLU11I      398  ACATGT               A^CATGT
BsrFI          99  GCCGGT               [GA]^CCGG[CT]
BstYI         318  AGATCT               [GA]^GATC[CT]
Cac8I         240  GCCAGC               GC[ACGT]^[ACGT]GC
Cfr10I         99  GCCGGT               [GA]^CCGG[CT]
Cfr13I         97  GGGCC                G^G[ACGT]CC
DrdI          365  GACATTTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          463  CCATGG               C^C[GA][CT]GG
HapII         100  CCGG                 C^CGG
HgaI          157  GACGC                GACGC(5/10)
HpaII         100  CCGG                 C^CGG
MaeI          302  CTAG                 C^TAG
MflI          318  AGATCT               [GA]^GATC[CT]
MspA1I        242  CAGCTG               C[AC]G^C[GT]G
MspI          100  CCGG                 C^CGG
MwoI          930  GCTTATTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          463  CCATGG               C^CATGG
NdeI         1136  CATATG               CA^TATG
NlaIV          96  GGGGCC               GG[ACGT]^[ACGT]CC
NspI          398  ACATGT               [GA]CATG^[CT]
PvuII         242  CAGCTG               CAG^CTG
SacI         1089  GAGCTC               GAGCT^C
Sau96I         97  GGGCC                G^G[ACGT]CC
SexAI         209  ACCTGGT              A^CC[AT]GGT
SfaNI         939  GCATC                GCATC(5/9)
SspI          345  AATATT               AAT^ATT
Tsp45I         41  GTCAC                ^GT[GC]AC
XhoII         318  AGATCT               [GA]^GATC[CT]
XmnI         1096  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BanII         274  GAGCCC               G[GA]GC[CT]^C
             1089  GAGCTC              
BclI          162  TGATCA               T^GATCA
              768  TGATCA              
BmyI          274  GAGCCC               G[AGT]GC[ACT]^C
             1089  GAGCTC              
BseRI          11  CTCCTC               GAGGAG(10/8)
               52  GAGGAG              
BsmAI         337  GAGAC                GTCTC(1/5)
              363  GAGAC               
Bsp1286I      274  GAGCCC               G[AGT]GC[ACT]^C
             1089  GAGCTC              
BstF5I        114  GGATG                GGATG(2/0)
              281  GGATG               
EcoT14I        92  CCAAGG               C^C[AT][AT]GG
              463  CCATGG              
EcoT22I        64  ATGCAT               ATGCA^T
              949  ATGCAT              
FbaI          162  TGATCA               T^GATCA
              768  TGATCA              
FokI          114  GGATG                GGATG(9/13)
              281  GGATG               
MaeIII         41  GTCAC                ^GT[ACGT]AC
              595  GTTAC               
NsiI           64  ATGCAT               ATGCA^T
              949  ATGCAT              
SmlI          134  CTCAAG               C^T[CT][GA]AG
              924  CTCAAG              
StyI           92  CCAAGG               C^C[AT][AT]GG
              463  CCATGG              
TaqI          600  TCGA                 T^CGA
             1188  TCGA                
TthHB8I       600  TCGA                 T^CGA
             1188  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AluI        ApoI        BsaJI       BsmI        
BsrI        BssKI       BstNI       DdeI        DpnII       
EarI        EcoRI       EcoRII      HaeIII      HinfI       
HphI        Ksp632I     MboI        MboII       MnlI        
MseI        MslI        MvaI        NdeII       NlaIII      
Sau3AI      ScrFI       StuI        TfiI        Tru9I       
Tsp509I     TspRI       
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