Restriction map for pF1KE0760
 (Linear) (Six-base) MAPSORT of: KIEE0760.seq  Check: 5787  from: 1  to: 1296



KIEE0760.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0     403    1296 
   Size:        403     893
 
AflIII A'CryG_T

Cuts at:      0     398    1296 
   Size:        398     898
 
AleI CACnn'nnGTG

Cuts at:      0     704     788    1208    1296 
   Size:        704      84     420      88

  Fragments arranged by size: 

                 704     420      88      84
 
ApoI r'AATT_y

Cuts at:      0     171     198     527     779     863    1031    1199    1283 
   Size:        171      27     329     252      84     168     168      84

Cuts at:   1283    1296 
   Size:         13

  Fragments arranged by size: 

                 329     252     171     168     168      84      84      27
                  13
 
BanII G_rGCy'C

Cuts at:      0     278    1093    1296 
   Size:        278     815     203

  Fragments arranged by size: 

                 815     278     203
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     401    1296 
   Size:        401     895
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      99    1296 
   Size:         99    1197
 
BclI T'GATC_A

Cuts at:      0     162     768    1296 
   Size:        162     606     528

  Fragments arranged by size: 

                 606     528     162
 
BfrBI ATG'CAT

Cuts at:      0      66     951    1296 
   Size:         66     885     345

  Fragments arranged by size: 

                 885     345      66
 
BglII A'GATC_T

Cuts at:      0     318    1296 
   Size:        318     978
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     909    1296 
   Size:        119     790     387

  Fragments arranged by size: 

                 790     387     119
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     331    1296 
   Size:        331     965
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       2      67    1296 
   Size:          2      65    1229

  Fragments arranged by size: 

                1229      65       2
 
BsiHKAI G_wGCw'C

Cuts at:      0    1093    1296 
   Size:       1093     203
 
BsmI GAATG_Cn'

Cuts at:      0      68     154     953    1296 
   Size:         68      86     799     343

  Fragments arranged by size: 

                 799     343      86      68
 
Bsp1286I G_dGCh'C

Cuts at:      0     278    1093    1296 
   Size:        278     815     203

  Fragments arranged by size: 

                 815     278     203
 
BsrFI r'CCGG_y

Cuts at:      0      99    1296 
   Size:         99    1197
 
BstYI r'GATC_y

Cuts at:      0     318    1296 
   Size:        318     978
 
BtgI C'CryG_G

Cuts at:      0     463    1296 
   Size:        463     833
 
BtsI GCAGTG_nn'

Cuts at:      0     988    1296 
   Size:        988     308
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     371    1296 
   Size:        371     925
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     355     449    1296 
   Size:         44     311      94     847

  Fragments arranged by size: 

                 847     311      94      44
 
EcoICRI GAG'CTC

Cuts at:      0    1091    1296 
   Size:       1091     205
 
EcoRI G'AATT_C

Cuts at:      0     527     779     863    1031    1199    1283    1296 
   Size:        527     252      84     168     168      84      13

  Fragments arranged by size: 

                 527     252     168     168      84      84      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     480     512    1296 
   Size:        310      32     138      32     784

  Fragments arranged by size: 

                 784     310     138      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     540    1296 
   Size:        173     367     756

  Fragments arranged by size: 

                 756     367     173
 
MslI CAynn'nnrTG

Cuts at:      0     468     536     704     788     872     956    1040    1124 
   Size:        468      68     168      84      84      84      84      84

Cuts at:   1124    1140    1208    1296 
   Size:         16      68      88

  Fragments arranged by size: 

                 468     168      88      84      84      84      84      84
                  68      68      16
 
MspA1I CmG'CkG

Cuts at:      0     244    1296 
   Size:        244    1052
 
NcoI C'CATG_G

Cuts at:      0     463    1296 
   Size:        463     833
 
NdeI CA'TA_TG

Cuts at:      0    1137    1296 
   Size:       1137     159
 
NsiI A_TGCA'T

Cuts at:      0      68     953    1296 
   Size:         68     885     343

  Fragments arranged by size: 

                 885     343      68
 
NspI r_CATG'y

Cuts at:      0     402    1296 
   Size:        402     894
 
PciI A'CATG_T

Cuts at:      0     398    1296 
   Size:        398     898
 
PvuII CAG'CTG

Cuts at:      0     244    1296 
   Size:        244    1052
 
SacI G_AGCT'C

Cuts at:      0    1093    1296 
   Size:       1093     203
 
SexAI A'CCwGG_T

Cuts at:      0     209    1296 
   Size:        209    1087
 
SmlI C'TyrA_G

Cuts at:      0     134     924    1296 
   Size:        134     790     372

  Fragments arranged by size: 

                 790     372     134
 
SspI AAT'ATT

Cuts at:      0     347    1296 
   Size:        347     949
 
StuI AGG'CCT

Cuts at:      0     308     994    1078    1162    1296 
   Size:        308     686      84      84     134

  Fragments arranged by size: 

                 686     308     134      84      84
 
StyI C'CwwG_G

Cuts at:      0      92     463    1296 
   Size:         92     371     833

  Fragments arranged by size: 

                 833     371      92
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      59    1296 
   Size:         59    1237
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     316     348     497     529    1296 
   Size:        316      32     149      32     767

  Fragments arranged by size: 

                 767     316     149      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1100    1296 
   Size:       1100     196
 

 Enzymes that do cut:

     AccI   AflIII     AleI     ApoI    BanII     BbsI    BciVI     BclI
    BfrBI    BglII    BpuEI     BsaI    BseRI  BsiHKAI     BsmI Bsp1286I
    BsrFI    BstYI     BtgI     BtsI     DrdI     EarI  EcoICRI    EcoRI
    Hin4I     MmeI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
     PciI    PvuII     SacI    SexAI     SmlI     SspI     StuI     StyI
    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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