Restriction map for pF1KE0759
 (Linear) (Six-base) MAPSORT of: KIEE0759.seq  Check: 4868  from: 1  to: 993



KIEE0759.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     724     993 
   Size:        724     269
 
AflIII A'CryG_T

Cuts at:      0     419     993 
   Size:        419     574
 
ApaLI G'TGCA_C

Cuts at:      0     887     993 
   Size:        887     106
 
ApoI r'AATT_y

Cuts at:      0     130     993 
   Size:        130     863
 
AseI AT'TA_AT

Cuts at:      0     709     993 
   Size:        709     284
 
AvaI C'yCGr_G

Cuts at:      0     215     993 
   Size:        215     778
 
BanII G_rGCy'C

Cuts at:      0     371     993 
   Size:        371     622
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     274     993 
   Size:        274     719
 
BlpI GC'TnA_GC

Cuts at:      0     692     993 
   Size:        692     301
 
Bme1580I G_kGCm'C

Cuts at:      0     891     993 
   Size:        891     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     963     993 
   Size:        313     650      30

  Fragments arranged by size: 

                 650     313      30
 
BsaAI yAC'GTr

Cuts at:      0      39     993 
   Size:         39     954
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     546     576     993 
   Size:        546      30     417

  Fragments arranged by size: 

                 546     417      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     316     567     993 
   Size:        316     251     426

  Fragments arranged by size: 

                 426     316     251
 
BseYI C'CCAG_C

Cuts at:      0     901     932     993 
   Size:        901      31      61

  Fragments arranged by size: 

                 901      61      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     412     993 
   Size:        169     243     581

  Fragments arranged by size: 

                 581     243     169
 
BsiEI CG_ry'CG

Cuts at:      0     564     993 
   Size:        564     429
 
BsiHKAI G_wGCw'C

Cuts at:      0     891     993 
   Size:        891     102
 
BsmI GAATG_Cn'

Cuts at:      0     466     993 
   Size:        466     527
 
Bsp1286I G_dGCh'C

Cuts at:      0     371     891     993 
   Size:        371     520     102

  Fragments arranged by size: 

                 520     371     102
 
BspHI T'CATG_A

Cuts at:      0     480     993 
   Size:        480     513
 
BssSI C'ACGA_G

Cuts at:      0     884     993 
   Size:        884     109
 
EaeI y'GGCC_r

Cuts at:      0     380     683     993 
   Size:        380     303     310

  Fragments arranged by size: 

                 380     310     303
 
EarI CTCTTCn'nnn_

Cuts at:      0     791     920     993 
   Size:        791     129      73

  Fragments arranged by size: 

                 791     129      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     724     963     993 
   Size:        313     411     239      30

  Fragments arranged by size: 

                 411     313     239      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     112     993 
   Size:        112     881
 
EcoRV GAT'ATC

Cuts at:      0     504     993 
   Size:        504     489
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     247     279     522     546     554     578     638     670 
   Size:        247      32     243      24       8      24      60      32

Cuts at:    670     993 
   Size:        323

  Fragments arranged by size: 

                 323     247     243      60      32      32      24      24
                   8
 
HindIII A'AGCT_T

Cuts at:      0      58     747     993 
   Size:         58     689     246

  Fragments arranged by size: 

                 689     246      58
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     993 
   Size:         32     961
 
MscI TGG'CCA

Cuts at:      0     685     993 
   Size:        685     308
 
MspA1I CmG'CkG

Cuts at:      0     396     993 
   Size:        396     597
 
NspI r_CATG'y

Cuts at:      0     235     993 
   Size:        235     758
 
PpuMI rG'GwC_Cy

Cuts at:      0     112     993 
   Size:        112     881
 
SfcI C'TryA_G

Cuts at:      0     843     972     993 
   Size:        843     129      21

  Fragments arranged by size: 

                 843     129      21
 
SphI G_CATG'C

Cuts at:      0     235     993 
   Size:        235     758
 
StyI C'CwwG_G

Cuts at:      0     360     515     993 
   Size:        360     155     478

  Fragments arranged by size: 

                 478     360     155
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     559     591     993 
   Size:        559      32     402

  Fragments arranged by size: 

                 559     402      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      22     993 
   Size:         22     971
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     522     993 
   Size:        522     471
 
XmnI GAAnn'nnTTC

Cuts at:      0     131     993 
   Size:        131     862
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI     AseI     AvaI    BanII     BbsI
     BlpI Bme1580I     BpmI    BsaAI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BssSI     EaeI     EarI
  Eco57MI EcoO109I    EcoRV    Hin4I  HindIII     MmeI     MscI   MspA1I
     NspI    PpuMI     SfcI     SphI     StyI     TstI  Tth111I     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TatI     XbaI
     XhoI     XmaI     ZraI
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