Restriction map for pF1KE0757
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BseRI       BsiWI       BsmBI       
BsmI        BspEI       BspLU11I    BspMI       BsrDI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CpoI        DraI        DraII       DraIII      
Eam1105I    EarI        EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        Ksp632I     MfeI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NarI        NcoI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SapI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SspI        SwaI        TfiI        Tru9I       Tsp509I     
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          550  GTCGAC               GT^[AC][GT]AC
Alw44I        762  GTGCAC               G^TGCAC
Aor51HI       505  AGCGCT               AGC^GCT
ApaLI         762  GTGCAC               G^TGCAC
AvaII          93  GGTCC                G^G[AT]CC
BamHI         302  GGATCC               G^GATCC
BfaI          652  CTAG                 C^TAG
BglI           47  GCCTGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          735  GCTGAGC              GC^T[ACGT]AGC
BpmI          187  CTGGAG               CTGGAG(16/14)
Bpu1102I      735  GCTGAGC              GC^T[ACGT]AGC
BsaBI         303  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          137  GAGACC               GGTCTC(1/5)
BsgI          840  GTGCAG               GTGCAG(16/14)
BsiEI          57  CGGCCG               CG[GA][CT]^CG
Bsp1407I      569  TGTACA               T^GTACA
BspDI         310  ATCGAT               AT^CGAT
BspHI         176  TCATGA               T^CATGA
BsrFI         658  GCCGGC               [GA]^CCGG[CT]
BsrGI         569  TGTACA               T^GTACA
BstXI         716  CCAGCAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         735  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        658  GCCGGC               [GA]^CCGG[CT]
ClaI          310  ATCGAT               AT^CGAT
DrdI          541  GACTGCAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           57  CGGCCG               C^GGCCG
EclXI          57  CGGCCG               C^GGCCG
Eco47III      505  AGCGCT               AGC^GCT
Eco52I         57  CGGCCG               C^GGCCG
EcoNI         141  CCTGATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII        550  GTCGAC               GT[CT]^[GA]AC
HindII        550  GTCGAC               GT[CT]^[GA]AC
HinfI         548  GAGTC                G^A[ACGT]TC
MaeI          652  CTAG                 C^TAG
MaeII         950  ACGT                 A^CGT
MamI          303  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         379  GAAGA                GAAGA(8/7)
NaeI          658  GCCGGC               GCC^GGC
NdeI          407  CATATG               CA^TATG
NgoMIV        658  GCCGGC               G^CCGGC
PflMI         376  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          548  GAGTC                GAGTC(4/5)
RcaI          176  TCATGA               T^CATGA
SacI          172  GAGCTC               GAGCT^C
SalI          550  GTCGAC               G^TCGAC
SbfI          744  CCTGCAGG             CCTGCA^GG
SmaI          679  CCCGGG               CCC^GGG
SphI          647  GCATGC               GCATG^C
Sse8387I      744  CCTGCAGG             CCTGCA^GG
SspBI         569  TGTACA               T^GTACA
Van91I        376  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         679  CCCGGG               C^CCGGG
XmaI          679  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          570  GTAC                 GT^AC
              963  GTAC                
AocI          247  CCTGAGG              CC^T[ACGT]AGG
              356  CCTCAGG             
BanI          361  GGCACC               G^G[CT][GA]CC
              790  GGCACC              
BsmAI         137  GAGAC                GTCTC(1/5)
              492  GTCTC               
BsrBI         738  GAGCGG               CCGCTC(-3/-3)
              892  GAGCGG              
BssHII         71  GCGCGC               G^CGCGC
              922  GCGCGC              
BstF5I        823  CATCC                GGATG(2/0)
              575  GGATG               
Bsu36I        247  CCTGAGG              CC^T[ACGT]AGG
              356  CCTCAGG             
Eco81I        247  CCTGAGG              CC^T[ACGT]AGG
              356  CCTCAGG             
EcoT14I       917  CCAAGG               C^C[AT][AT]GG
              974  CCAAGG              
FokI          823  CATCC                GGATG(9/13)
              575  GGATG               
HaeII         505  AGCGCT               [GA]GCGC^[CT]
              727  GGCGCT              
KspI          529  CCGCGG               CCGC^GG
              605  CCGCGG              
NspI          486  GCATGT               [GA]CATG^[CT]
              647  GCATGC              
PstI          543  CTGCAG               CTGCA^G
              745  CTGCAG              
RsaI          570  GTAC                 GT^AC
              963  GTAC                
SacII         529  CCGCGG               CCGC^GG
              605  CCGCGG              
SfcI          543  CTGCAG               C^T[GA][CT]AG
              745  CTGCAG              
SmlI          703  CTCAAG               C^T[CT][GA]AG
              862  CTCAAG              
StuI          244  AGGCCT               AGG^CCT
              522  AGGCCT              
StyI          917  CCAAGG               C^C[AT][AT]GG
              974  CCAAGG              
TaqI          311  TCGA                 T^CGA
              551  TCGA                
Tsp45I         13  GTGAC                ^GT[GC]AC
              845  GTGAC               
TthHB8I       311  TCGA                 T^CGA
              551  TCGA                
XcmI          147  CCAGGCCATCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              968  CCAATGCCAAGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AspHI       AvaI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstAPI      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EcoRII      FauI        Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MaeIII      MboI        MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        TspRI       
XhoII       
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