Restriction map for pF1KE0757
 (Linear) (Six-base) MAPSORT of: KIEE0757.seq  Check: 5562  from: 1  to: 990



KIEE0757.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0     551     990 
   Size:        551     439
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     990 
   Size:         51     939
 
AfeI AGC'GCT

Cuts at:      0     507     990 
   Size:        507     483
 
AlwNI CAG_nnn'CTG

Cuts at:      0     260     753     848     990 
   Size:        260     493      95     142

  Fragments arranged by size: 

                 493     260     142      95
 
ApaLI G'TGCA_C

Cuts at:      0     762     990 
   Size:        762     228
 
AvaI C'yCGr_G

Cuts at:      0     679     757     826     937     990 
   Size:        679      78      69     111      53

  Fragments arranged by size: 

                 679     111      78      69      53
 
BamHI G'GATC_C

Cuts at:      0     302     990 
   Size:        302     688
 
BanI G'GyrC_C

Cuts at:      0     361     790     990 
   Size:        361     429     200

  Fragments arranged by size: 

                 429     361     200
 
BanII G_rGCy'C

Cuts at:      0     176     562     599     614     990 
   Size:        176     386      37      15     376

  Fragments arranged by size: 

                 386     376     176      37      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0      53     990 
   Size:         53     937
 
BlpI GC'TnA_GC

Cuts at:      0     736     990 
   Size:        736     254
 
Bme1580I G_kGCm'C

Cuts at:      0     133     273     766     793     990 
   Size:        133     140     493      27     197

  Fragments arranged by size: 

                 493     197     140     133      27
 
BmgBI CAC'GTC

Cuts at:      0     951     990 
   Size:        951      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     260     380     990 
   Size:        260     120     610

  Fragments arranged by size: 

                 610     260     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     990 
   Size:        208     782
 
Bpu10I CC'TnA_GC

Cuts at:      0     290     990 
   Size:        290     700
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688     847     990 
   Size:        688     159     143

  Fragments arranged by size: 

                 688     159     143
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     131     990 
   Size:        131     859
 
BsaBI GATnn'nnATC

Cuts at:      0     307     990 
   Size:        307     683
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     544     574     990 
   Size:        544      30     416

  Fragments arranged by size: 

                 544     416      30
 
BseYI C'CCAG_C

Cuts at:      0     502     513     723     990 
   Size:        502      11     210     267

  Fragments arranged by size: 

                 502     267     210      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     861     990 
   Size:        861     129
 
BsiEI CG_ry'CG

Cuts at:      0      60     990 
   Size:         60     930
 
BsiHKAI G_wGCw'C

Cuts at:      0     169     176     481     766     990 
   Size:        169       7     305     285     224

  Fragments arranged by size: 

                 305     285     224     169       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     133     169     176     273     481     562     599     614 
   Size:        133      36       7      97     208      81      37      15

Cuts at:    614     766     793     990 
   Size:        152      27     197

  Fragments arranged by size: 

                 208     197     152     133      97      81      37      36
                  27      15       7
 
BspHI T'CATG_A

Cuts at:      0     176     990 
   Size:        176     814
 
BsrBI CCG'CTC

Cuts at:      0     740     894     990 
   Size:        740     154      96

  Fragments arranged by size: 

                 740     154      96
 
BsrFI r'CCGG_y

Cuts at:      0     658     990 
   Size:        658     332
 
BsrGI T'GTAC_A

Cuts at:      0     569     990 
   Size:        569     421
 
BssHII G'CGCG_C

Cuts at:      0      71     922     990 
   Size:         71     851      68

  Fragments arranged by size: 

                 851      71      68
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     121     648     753     770     848     990 
   Size:        121     527     105      17      78     142

  Fragments arranged by size: 

                 527     142     121     105      78      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     723     990 
   Size:        723     267
 
BstYI r'GATC_y

Cuts at:      0     302     320     400     990 
   Size:        302      18      80     590

  Fragments arranged by size: 

                 590     302      80      18
 
Bsu36I CC'TnA_GG

Cuts at:      0     248     357     990 
   Size:        248     109     633

  Fragments arranged by size: 

                 633     248     109
 
BtgI C'CryG_G

Cuts at:      0     529     599     605     710     777     990 
   Size:        529      70       6     105      67     213

  Fragments arranged by size: 

                 529     213     105      70      67       6
 
BtsI GCAGTG_nn'

Cuts at:      0     849     990 
   Size:        849     141
 
ClaI AT'CG_AT

Cuts at:      0     311     990 
   Size:        311     679
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     852     887     990 
   Size:        852      35     103

  Fragments arranged by size: 

                 852     103      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     547     990 
   Size:        547     443
 
EaeI y'GGCC_r

Cuts at:      0      57     602     713     990 
   Size:         57     545     111     277

  Fragments arranged by size: 

                 545     277     111      57
 
EagI C'GGCC_G

Cuts at:      0      57     990 
   Size:         57     933
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      34     990 
   Size:         34     956
 
EcoICRI GAG'CTC

Cuts at:      0     174     990 
   Size:        174     816
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     208     990 
   Size:         51     157     782

  Fragments arranged by size: 

                 782     157      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     145     990 
   Size:        145     845
 
HaeII r_GCGC'y

Cuts at:      0     509     731     990 
   Size:        509     222     259

  Fragments arranged by size: 

                 509     259     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     128     160     544     576     990 
   Size:        128      32     384      32     414

  Fragments arranged by size: 

                 414     384     128      32      32
 
HincII GTy'rAC

Cuts at:      0     552     990 
   Size:        552     438
 
MspA1I CmG'CkG

Cuts at:      0     531     607     735     990 
   Size:        531      76     128     255

  Fragments arranged by size: 

                 531     255     128      76
 
NaeI GCC'GGC

Cuts at:      0     660     990 
   Size:        660     330
 
NdeI CA'TA_TG

Cuts at:      0     408     990 
   Size:        408     582
 
NgoMIV G'CCGG_C

Cuts at:      0     658     990 
   Size:        658     332
 
NspI r_CATG'y

Cuts at:      0     490     651     990 
   Size:        490     161     339

  Fragments arranged by size: 

                 490     339     161
 
PasI CC'CwG_GG

Cuts at:      0      35     990 
   Size:         35     955
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     382     990 
   Size:        382     608
 
PfoI T'CCnGG_A

Cuts at:      0     323     990 
   Size:        323     667
 
PstI C_TGCA'G

Cuts at:      0     547     749     990 
   Size:        547     202     241

  Fragments arranged by size: 

                 547     241     202
 
SacI G_AGCT'C

Cuts at:      0     176     990 
   Size:        176     814
 
SacII CC_GC'GG

Cuts at:      0     532     608     990 
   Size:        532      76     382

  Fragments arranged by size: 

                 532     382      76
 
SalI G'TCGA_C

Cuts at:      0     550     990 
   Size:        550     440
 
SbfI CC_TGCA'GG

Cuts at:      0     749     990 
   Size:        749     241
 
SfcI C'TryA_G

Cuts at:      0     543     745     990 
   Size:        543     202     245

  Fragments arranged by size: 

                 543     245     202
 
SmaI CCC'GGG

Cuts at:      0     681     990 
   Size:        681     309
 
SmlI C'TyrA_G

Cuts at:      0     703     862     990 
   Size:        703     159     128

  Fragments arranged by size: 

                 703     159     128
 
SphI G_CATG'C

Cuts at:      0     651     990 
   Size:        651     339
 
SrfI GCCC'GGGC

Cuts at:      0     681     990 
   Size:        681     309
 
StuI AGG'CCT

Cuts at:      0     246     524     990 
   Size:        246     278     466

  Fragments arranged by size: 

                 466     278     246
 
StyI C'CwwG_G

Cuts at:      0     917     974     990 
   Size:        917      57      16

  Fragments arranged by size: 

                 917      57      16
 
TatI w'GTAC_w

Cuts at:      0     569     990 
   Size:        569     421
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     287     319     990 
   Size:        287      32     671

  Fragments arranged by size: 

                 671     287      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     154     975     990 
   Size:        154     821      15

  Fragments arranged by size: 

                 821     154      15
 
XmaI C'CCGG_G

Cuts at:      0     679     990 
   Size:        679     311
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AlwNI    ApaLI     AvaI    BamHI     BanI
    BanII     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI    HaeII    Hin4I   HincII
   MspA1I     NaeI     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI
     PstI     SacI    SacII     SalI     SbfI     SfcI     SmaI     SmlI
     SphI     SrfI     StuI     StyI     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BmtI     BplI    BsaAI    BsaHI    BsaWI    BseRI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BssSI    BstBI
   BstEII  BstZ17I    BtgZI     DraI   DraIII     EarI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmnI     ZraI
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