Restriction map for pF1KE0742
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MroI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwNI        1042  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI           28  CCCGAG               C^[CT]CG[GA]G
BbrPI         275  CACGTG               CAC^GTG
BbsI          762  GAAGAC               GAAGAC(2/6)
BfaI          965  CTAG                 C^TAG
BpmI         1019  CTGGAG               CTGGAG(16/14)
BpuAI         762  GAAGAC               GAAGAC(2/6)
BsaBI         702  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         617  TCCGGT               [AT]^CCGG[AT]
BseRI        1137  CTCCTC               GAGGAG(10/8)
BsoBI          28  CCCGAG               C^[CT]CG[GA]G
BspLU11I      836  ACATGT               A^CATGT
BsrFI         917  ACCGGC               [GA]^CCGG[CT]
BstAPI        597  GCAGATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         824  CCAACTCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        917  ACCGGC               [GA]^CCGG[CT]
DraIII       1208  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1057  CCGTGG               C^C[GA][CT]GG
EarI         1080  CTCTTC               CTCTTC(1/4)
EcoNI         978  CCTGGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         843  GGCGCT               [GA]GCGC^[CT]
Ksp632I      1080  CTCTTC               CTCTTC(1/4)
MaeI          965  CTAG                 C^TAG
MamI          702  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1174  ACGCGT               A^CGCGT
MseI         1127  TTAA                 T^TAA
PflMI         468  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         275  CACGTG               CAC^GTG
PmlI          275  CACGTG               CAC^GTG
PpuMI          20  AGGTCCT              [GA]G^G[AT]CC[CT]
SphI          627  GCATGC               GCATG^C
TaqI          485  TCGA                 T^CGA
TfiI          666  GAATC                G^A[AT]TC
Tru9I        1127  TTAA                 T^TAA
Tsp509I       309  AATT                 ^AATT
TthHB8I       485  TCGA                 T^CGA
Van91I        468  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwI          924  GATCC                GGATC(4/5)
               54  GGATC               
AocI          927  CCTCAGG              CC^T[ACGT]AGG
             1047  CCTGAGG             
BanI          877  GGCACC               G^G[CT][GA]CC
             1052  GGCACC              
BsaAI         275  CACGTG               [CT]AC^GT[GA]
              338  TACGTG              
BsaI          388  GAGACC               GGTCTC(1/5)
              650  GAGACC              
BsgI         1034  CTGCAC               GTGCAG(16/14)
             1099  CTGCAC              
BsmFI          10  GGGAC                GGGAC(10/14)
              191  GTCCC               
Bsp1407I      101  TGTACA               T^GTACA
              503  TGTACA              
BspMI        1097  ACCTGC               ACCTGC(4/8)
             1244  ACCTGC              
BsrGI         101  TGTACA               T^GTACA
              503  TGTACA              
BstYI          54  GGATCT               [GA]^GATC[CT]
              923  AGATCC              
Bsu36I        927  CCTCAGG              CC^T[ACGT]AGG
             1047  CCTGAGG             
Eco81I        927  CCTCAGG              CC^T[ACGT]AGG
             1047  CCTGAGG             
MflI           54  GGATCT               [GA]^GATC[CT]
              923  AGATCC              
MspA1I         49  CAGCTG               C[AC]G^C[GT]G
              469  CAGCTG              
NspI          627  GCATGC               [GA]CATG^[CT]
              836  ACATGT              
PleI          694  GACTC                GAGTC(4/5)
              421  GAGTC               
PstI          595  CTGCAG               CTGCA^G
             1039  CTGCAG              
PvuII          49  CAGCTG               CAG^CTG
              469  CAGCTG              
SacI          535  GAGCTC               GAGCT^C
             1237  GAGCTC              
SfaNI         548  GCATC                GCATC(5/9)
              638  GCATC               
SfcI          595  CTGCAG               C^T[GA][CT]AG
             1039  CTGCAG              
SspBI         101  TGTACA               T^GTACA
              503  TGTACA              
XhoII          54  GGATCT               [GA]^GATC[CT]
              923  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AspHI       AvaII       BalI        BanII       
BbvI        BcnI        BmyI        BsaHI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrBI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
StyI        TseI        Tsp45I      TspRI       
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