Restriction map for pF1KE0742
 (Linear) (Six-base) MAPSORT of: KIEE0742.seq  Check: 7802  from: 1  to: 1272



KIEE0742.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     309    1272 
   Size:         63     246     963

  Fragments arranged by size: 

                 963     246      63
 
AflIII A'CryG_T

Cuts at:      0      98     276     339     836    1174    1272 
   Size:         98     178      63     497     338      98

  Fragments arranged by size: 

                 497     338     178      98      98      63
 
AleI CACnn'nnGTG

Cuts at:      0     121     589    1272 
   Size:        121     468     683

  Fragments arranged by size: 

                 683     468     121
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     198     386    1272 
   Size:        198     188     886

  Fragments arranged by size: 

                 886     198     188
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1047    1272 
   Size:       1047     225
 
AvaI C'yCGr_G

Cuts at:      0      28    1272 
   Size:         28    1244
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     888     921    1272 
   Size:        888      33     351

  Fragments arranged by size: 

                 888     351      33
 
BanI G'GyrC_C

Cuts at:      0     877    1052    1272 
   Size:        877     175     220

  Fragments arranged by size: 

                 877     220     175
 
BanII G_rGCy'C

Cuts at:      0      77     539     565     959    1026    1241    1272 
   Size:         77     462      26     394      67     215      31

  Fragments arranged by size: 

                 462     394     215      77      67      31      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     769    1272 
   Size:        769     503
 
Bme1580I G_kGCm'C

Cuts at:      0     691    1272 
   Size:        691     581
 
BmgBI CAC'GTC

Cuts at:      0      99    1272 
   Size:         99    1173
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     643    1161    1272 
   Size:        643     518     111

  Fragments arranged by size: 

                 643     518     111
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     237    1272 
   Size:        237    1035
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1040    1272 
   Size:       1040     232
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     382     644    1272 
   Size:        382     262     628

  Fragments arranged by size: 

                 628     382     262
 
BsaAI yAC'GTr

Cuts at:      0     277     340    1272 
   Size:        277      63     932

  Fragments arranged by size: 

                 932     277      63
 
BsaBI GATnn'nnATC

Cuts at:      0     706    1272 
   Size:        706     566
 
BsaHI Gr'CG_yC

Cuts at:      0     581     614     851    1272 
   Size:        581      33     237     421

  Fragments arranged by size: 

                 581     421     237      33
 
BsaWI w'CCGG_w

Cuts at:      0     617    1272 
   Size:        617     655
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     201     231     487     517    1272 
   Size:        201      30     256      30     755

  Fragments arranged by size: 

                 755     256     201      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1128    1272 
   Size:       1128     144
 
BseYI C'CCAG_C

Cuts at:      0     156     846    1272 
   Size:        156     690     426

  Fragments arranged by size: 

                 690     426     156
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1019    1084    1272 
   Size:       1019      65     188

  Fragments arranged by size: 

                1019     188      65
 
BsiHKAI G_wGCw'C

Cuts at:      0     350     539     735    1156    1241    1272 
   Size:        350     189     196     421      85      31

  Fragments arranged by size: 

                 421     350     196     189      85      31
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     350     539     565     691     735     959    1026 
   Size:         77     273     189      26     126      44     224      67

Cuts at:   1026    1156    1241    1272 
   Size:        130      85      31

  Fragments arranged by size: 

                 273     224     189     130     126      85      77      67
                  44      31      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1106    1253    1272 
   Size:       1106     147      19

  Fragments arranged by size: 

                1106     147      19
 
BsrBI CCG'CTC

Cuts at:      0     498    1106    1187    1272 
   Size:        498     608      81      85

  Fragments arranged by size: 

                 608     498      85      81
 
BsrDI GCAATG_nn'

Cuts at:      0     551     788     854    1272 
   Size:        551     237      66     418

  Fragments arranged by size: 

                 551     418     237      66
 
BsrFI r'CCGG_y

Cuts at:      0     917    1272 
   Size:        917     355
 
BsrGI T'GTAC_A

Cuts at:      0     101     503    1272 
   Size:        101     402     769

  Fragments arranged by size: 

                 769     402     101
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     603    1272 
   Size:        603     669
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     831    1272 
   Size:        831     441
 
BstYI r'GATC_y

Cuts at:      0      54     923    1272 
   Size:         54     869     349

  Fragments arranged by size: 

                 869     349      54
 
Bsu36I CC'TnA_GG

Cuts at:      0     928    1048    1272 
   Size:        928     120     224

  Fragments arranged by size: 

                 928     224     120
 
BtgI C'CryG_G

Cuts at:      0    1057    1272 
   Size:       1057     215
 
BtsI GCAGTG_nn'

Cuts at:      0    1036    1272 
   Size:       1036     236
 
DraIII CAC_nnn'GTG

Cuts at:      0    1213    1272 
   Size:       1213      59
 
EaeI y'GGCC_r

Cuts at:      0     204     312     521     725     980    1272 
   Size:        204     108     209     204     255     292

  Fragments arranged by size: 

                 292     255     209     204     204     108
 
EarI CTCTTCn'nnn_

Cuts at:      0    1086    1272 
   Size:       1086     186
 
EcoICRI GAG'CTC

Cuts at:      0     537    1239    1272 
   Size:        537     702      33

  Fragments arranged by size: 

                 702     537      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     309    1040    1272 
   Size:         63     246     731     232

  Fragments arranged by size: 

                 731     246     232      63
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     982    1272 
   Size:        982     290
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     303    1044    1272 
   Size:         21     282     741     228

  Fragments arranged by size: 

                 741     282     228      21
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     582    1272 
   Size:        582     690
 
HaeII r_GCGC'y

Cuts at:      0     847    1272 
   Size:        847     425
 
MluI A'CGCG_T

Cuts at:      0    1174    1272 
   Size:       1174      98
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     998    1272 
   Size:        404     594     274

  Fragments arranged by size: 

                 594     404     274
 
MscI TGG'CCA

Cuts at:      0     206     523     727     982    1272 
   Size:        206     317     204     255     290

  Fragments arranged by size: 

                 317     290     255     206     204
 
MslI CAynn'nnrTG

Cuts at:      0     121     379     511     589    1272 
   Size:        121     258     132      78     683

  Fragments arranged by size: 

                 683     258     132     121      78
 
MspA1I CmG'CkG

Cuts at:      0      51     471    1272 
   Size:         51     420     801

  Fragments arranged by size: 

                 801     420      51
 
NspI r_CATG'y

Cuts at:      0     631     840    1272 
   Size:        631     209     432

  Fragments arranged by size: 

                 631     432     209
 
PciI A'CATG_T

Cuts at:      0     836    1272 
   Size:        836     436
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     474    1272 
   Size:        474     798
 
PmlI CAC'GTG

Cuts at:      0     277    1272 
   Size:        277     995
 
PpuMI rG'GwC_Cy

Cuts at:      0      21    1272 
   Size:         21    1251
 
PstI C_TGCA'G

Cuts at:      0     599    1043    1272 
   Size:        599     444     229

  Fragments arranged by size: 

                 599     444     229
 
PvuII CAG'CTG

Cuts at:      0      51     471    1272 
   Size:         51     420     801

  Fragments arranged by size: 

                 801     420      51
 
SacI G_AGCT'C

Cuts at:      0     539    1241    1272 
   Size:        539     702      31

  Fragments arranged by size: 

                 702     539      31
 
SfcI C'TryA_G

Cuts at:      0     595    1039    1272 
   Size:        595     444     233

  Fragments arranged by size: 

                 595     444     233
 
SphI G_CATG'C

Cuts at:      0     631    1272 
   Size:        631     641
 
StyI C'CwwG_G

Cuts at:      0      38     557     983    1272 
   Size:         38     519     426     289

  Fragments arranged by size: 

                 519     426     289      38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      51    1050    1272 
   Size:         51     999     222

  Fragments arranged by size: 

                 999     222      51
 
TatI w'GTAC_w

Cuts at:      0     101     254     503     785     938    1272 
   Size:        101     153     249     282     153     334

  Fragments arranged by size: 

                 334     282     249     153     153     101
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     201     233    1272 
   Size:        201      32    1039

  Fragments arranged by size: 

                1039     201      32
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     AvaI     BaeI     BanI
    BanII     BbsI Bme1580I    BmgBI     BmrI     BplI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII     MluI     MmeI
     MscI     MslI   MspA1I     NspI     PciI    PflMI     PmlI    PpuMI
     PstI    PvuII     SacI     SfcI     SphI     StyI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmtI   Bpu10I    BpuEI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI   BssHII    BssSI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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