Restriction map for pF1KE0733
 (Linear) (Six-base) MAPSORT of: KIEE0733.seq  Check: 2284  from: 1  to: 933



KIEE0733.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     265     933 
   Size:        265     668
 
ApaI G_GGCC'C

Cuts at:      0     400     933 
   Size:        400     533
 
AvaI C'yCGr_G

Cuts at:      0      37     346     933 
   Size:         37     309     587

  Fragments arranged by size: 

                 587     309      37
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     622     655     933 
   Size:        622      33     278

  Fragments arranged by size: 

                 622     278      33
 
BanI G'GyrC_C

Cuts at:      0     130     284     556     794     933 
   Size:        130     154     272     238     139

  Fragments arranged by size: 

                 272     238     154     139     130
 
BanII G_rGCy'C

Cuts at:      0     400     746     933 
   Size:        400     346     187

  Fragments arranged by size: 

                 400     346     187
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     129     933 
   Size:        129     804
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     120     154     933 
   Size:        120      34     779

  Fragments arranged by size: 

                 779     120      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     913     933 
   Size:        913      20
 
BlpI GC'TnA_GC

Cuts at:      0     740     933 
   Size:        740     193
 
Bme1580I G_kGCm'C

Cuts at:      0     287     400     561     783     933 
   Size:        287     113     161     222     150

  Fragments arranged by size: 

                 287     222     161     150     113
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     412     679     933 
   Size:        412     267     254

  Fragments arranged by size: 

                 412     267     254
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     642     933 
   Size:        642     291
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     933 
   Size:        151     782
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     670     933 
   Size:        670     263
 
BseYI C'CCAG_C

Cuts at:      0     204     753     933 
   Size:        204     549     180

  Fragments arranged by size: 

                 549     204     180
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     933 
   Size:        293     640
 
BsiHKAI G_wGCw'C

Cuts at:      0     746     933 
   Size:        746     187
 
Bsp1286I G_dGCh'C

Cuts at:      0     287     400     561     746     783     933 
   Size:        287     113     161     185      37     150

  Fragments arranged by size: 

                 287     185     161     150     113      37
 
BspHI T'CATG_A

Cuts at:      0     567     933 
   Size:        567     366
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     265     933 
   Size:        265     668
 
BsrBI CCG'CTC

Cuts at:      0     351     933 
   Size:        351     582
 
BsrFI r'CCGG_y

Cuts at:      0     791     933 
   Size:        791     142
 
BsrGI T'GTAC_A

Cuts at:      0     605     933 
   Size:        605     328
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     469     933 
   Size:        469     464
 
BstYI r'GATC_y

Cuts at:      0     369     623     836     933 
   Size:        369     254     213      97

  Fragments arranged by size: 

                 369     254     213      97
 
BtgI C'CryG_G

Cuts at:      0     468     522     933 
   Size:        468      54     411

  Fragments arranged by size: 

                 468     411      54
 
BtsI GCAGTG_nn'

Cuts at:      0     253     456     933 
   Size:        253     203     477

  Fragments arranged by size: 

                 477     253     203
 
DraIII CAC_nnn'GTG

Cuts at:      0     406     933 
   Size:        406     527
 
EaeI y'GGCC_r

Cuts at:      0     173     432     653     933 
   Size:        173     259     221     280

  Fragments arranged by size: 

                 280     259     221     173
 
EarI CTCTTCn'nnn_

Cuts at:      0     827     933 
   Size:        827     106
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     289     778     933 
   Size:        289     489     155

  Fragments arranged by size: 

                 489     289     155
 
EcoICRI GAG'CTC

Cuts at:      0     744     933 
   Size:        744     189
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     933 
   Size:        151     782
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     714     933 
   Size:        115     599     219

  Fragments arranged by size: 

                 599     219     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0      22      41     396     901     933 
   Size:         22      19     355     505      32

  Fragments arranged by size: 

                 505     355      32      22      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     141     933 
   Size:        141     792
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     137     169     933 
   Size:         29     108      32     764

  Fragments arranged by size: 

                 764     108      32      29
 
HincII GTy'rAC

Cuts at:      0       5     933 
   Size:          5     928
 
MscI TGG'CCA

Cuts at:      0     175     434     933 
   Size:        175     259     499

  Fragments arranged by size: 

                 499     259     175
 
MslI CAynn'nnrTG

Cuts at:      0     181     258     467     933 
   Size:        181      77     209     466

  Fragments arranged by size: 

                 466     209     181      77
 
MspA1I CmG'CkG

Cuts at:      0     422     933 
   Size:        422     511
 
NspI r_CATG'y

Cuts at:      0     239     933 
   Size:        239     694
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     441     662     933 
   Size:        441     221     271

  Fragments arranged by size: 

                 441     271     221
 
PpuMI rG'GwC_Cy

Cuts at:      0      41     933 
   Size:         41     892
 
PspOMI G'GGCC_C

Cuts at:      0     396     933 
   Size:        396     537
 
PspXI vC'TCGA_Gb

Cuts at:      0     346     933 
   Size:        346     587
 
SacI G_AGCT'C

Cuts at:      0     746     933 
   Size:        746     187
 
SanDI GG'GwC_CC

Cuts at:      0      41     933 
   Size:         41     892
 
SfcI C'TryA_G

Cuts at:      0      16     933 
   Size:         16     917
 
SgrAI Cr'CCGG_yG

Cuts at:      0     791     933 
   Size:        791     142
 
SmaI CCC'GGG

Cuts at:      0      39     933 
   Size:         39     894
 
SmlI C'TyrA_G

Cuts at:      0     346     933 
   Size:        346     587
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     259     394     933 
   Size:        259     135     539

  Fragments arranged by size: 

                 539     259     135
 
TatI w'GTAC_w

Cuts at:      0     605     933 
   Size:        605     328
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     255     287     448     480     659     691     933 
   Size:        255      32     161      32     179      32     242

  Fragments arranged by size: 

                 255     242     179     161      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     229     933 
   Size:        229     704
 
XhoI C'TCGA_G

Cuts at:      0     346     933 
   Size:        346     587
 
XmaI C'CCGG_G

Cuts at:      0      37     933 
   Size:         37     896
 

 Enzymes that do cut:

     AarI     ApaI     AvaI     BaeI     BanI    BanII     BbsI     BcgI
     BglI     BlpI Bme1580I     BmrI     BplI     BpmI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII     MscI
     MslI   MspA1I     NspI    PflMI    PpuMI   PspOMI    PspXI     SacI
    SanDI     SfcI    SgrAI     SmaI     SmlI    TaqII     TatI     TstI
  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII    BamHI     BbeI    BbvCI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     XbaI     XcmI     XmnI
     ZraI
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