Restriction map for pF1KE0729
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoT22I     FauI        FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NaeI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tsp45I      Tth111I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcsI          157  AAATTT               [GA]^AATT[CT]
Alw44I        565  GTGCAC               G^TGCAC
AlwNI         856  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          579  CCTGAGG              CC^T[ACGT]AGG
ApaI          794  GGGCCC               GGGCC^C
ApaLI         565  GTGCAC               G^TGCAC
ApoI          157  AAATTT               [GA]^AATT[CT]
AvaI          797  CCCGGG               C^[CT]CG[GA]G
BanI          107  GGCGCC               G^G[CT][GA]CC
BbeI          107  GGCGCC               GGCGC^C
BbsI          588  GAAGAC               GAAGAC(2/6)
BpmI          250  CTGGAG               CTGGAG(16/14)
BpuAI         588  GAAGAC               GAAGAC(2/6)
BsgI           22  GTGCAG               GTGCAG(16/14)
BsoBI         797  CCCGGG               C^[CT]CG[GA]G
BstAPI        255  GCACCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        750  CATCC                GGATG(2/0)
BstXI          11  CCAGAGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        579  CCTGAGG              CC^T[ACGT]AGG
DraII         793  GGGGCCC              [GA]G^G[ACGT]CC[CT]
Eco81I        579  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      793  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         704  GATATC               GAT^ATC
FokI          750  CATCC                GGATG(9/13)
KasI          107  GGCGCC               G^GCGCC
MseI          500  TTAA                 T^TAA
MslI          672  CATTGGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          107  GGCGCC               GG^CGCC
NdeI          644  CATATG               CA^TATG
PflMI         828  CCAGCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI           43  CCTGCAGG             CCTGCA^GG
SfoI          107  GGCGCC               GGC^GCC
SmaI          797  CCCGGG               CCC^GGG
Sse8387I       43  CCTGCAGG             CCTGCA^GG
TaqI          119  TCGA                 T^CGA
Tru9I         500  TTAA                 T^TAA
TthHB8I       119  TCGA                 T^CGA
Van91I        828  CCAGCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         797  CCCGGG               C^CCGGG
XmaI          797  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AciI          619  CCGC                 CCGC(-3/-1)
              105  GCGG                
AcyI          107  GGCGCC               G[GA]^CG[CT]C
              137  GGCGTC              
AfaI          171  GTAC                 GT^AC
              348  GTAC                
AlwI          279  GATCC                GGATC(4/5)
              858  GATCC               
BsaHI         107  GGCGCC               G[GA]^CG[CT]C
              137  GGCGTC              
BseRI         442  GAGGAG               GAGGAG(10/8)
              582  GAGGAG              
BsmAI         736  GAGAC                GTCTC(1/5)
              840  GAGAC               
BspMI          42  ACCTGC               ACCTGC(4/8)
              512  ACCTGC              
BsrI          249  ACTGG                ACTGG(1/-1)
              606  ACTGG               
BstYI         278  AGATCC               [GA]^GATC[CT]
              857  AGATCC              
Cac8I          46  GCAGGC               GC[ACGT]^[ACGT]GC
              337  GCACGC              
DpnII         279  GATC                 ^GATC
              858  GATC                
EarI          220  CTCTTC               CTCTTC(1/4)
               52  GAAGAG              
EcoNI         144  CCTGGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              575  CCTCCCTGAGG         
EcoT14I       370  CCAAGG               C^C[AT][AT]GG
              833  CCTTGG              
HaeII         107  GGCGCC               [GA]GCGC^[CT]
              721  AGCGCC              
HgaI          746  GACGC                GACGC(5/10)
              138  GCGTC               
Hin1I         107  GGCGCC               G[GA]^CG[CT]C
              137  GGCGTC              
HinfI         354  GATTC                G^A[ACGT]TC
              486  GATTC               
Ksp632I       220  CTCTTC               CTCTTC(1/4)
               52  GAAGAG              
MboI          279  GATC                 ^GATC
              858  GATC                
MflI          278  AGATCC               [GA]^GATC[CT]
              857  AGATCC              
MspA1I         25  CAGCTG               C[AC]G^C[GT]G
              259  CAGCTG              
NdeII         279  GATC                 ^GATC
              858  GATC                
PstI           44  CTGCAG               CTGCA^G
              394  CTGCAG              
PvuII          25  CAGCTG               CAG^CTG
              259  CAGCTG              
RsaI          171  GTAC                 GT^AC
              348  GTAC                
SacI            3  GAGCTC               GAGCT^C
              379  GAGCTC              
Sau3AI        279  GATC                 ^GATC
              858  GATC                
SmlI          184  CTCAAG               C^T[CT][GA]AG
              754  CTCAAG              
StyI          370  CCAAGG               C^C[AT][AT]GG
              833  CCTTGG              
TfiI          354  GATTC                G^A[AT]TC
              486  GATTC               
TspRI         605  CACTG                CA[GC]TG[ACGT][ACGT]^
              732  CAGTG               
XhoII         278  AGATCC               [GA]^GATC[CT]
              857  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        AspHI       BanII       BbvI        BcnI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BssKI       BstNI       CfoI        
Cfr13I      DdeI        EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       Sau96I      
ScrFI       SfaNI       SfcI        TseI        Tsp509I     
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