Restriction map for pF1KE0729
 (Linear) (Six-base) MAPSORT of: KIEE0729.seq  Check: 2118  from: 1  to: 927



KIEE0729.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     883     927 
   Size:        232     651      44

  Fragments arranged by size: 

                 651     232      44
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     694     927 
   Size:        694     233
 
AlwNI CAG_nnn'CTG

Cuts at:      0     861     927 
   Size:        861      66
 
ApaI G_GGCC'C

Cuts at:      0     798     927 
   Size:        798     129
 
ApaLI G'TGCA_C

Cuts at:      0     565     927 
   Size:        565     362
 
ApoI r'AATT_y

Cuts at:      0     157     927 
   Size:        157     770
 
AvaI C'yCGr_G

Cuts at:      0     797     927 
   Size:        797     130
 
BanI G'GyrC_C

Cuts at:      0     107     927 
   Size:        107     820
 
BanII G_rGCy'C

Cuts at:      0       7     383     798     927 
   Size:          7     376     415     129

  Fragments arranged by size: 

                 415     376     129       7
 
BbeI G_GCGC'C

Cuts at:      0     111     927 
   Size:        111     816
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     595     927 
   Size:        595     332
 
Bme1580I G_kGCm'C

Cuts at:      0     569     798     927 
   Size:        569     229     129

  Fragments arranged by size: 

                 569     229     129
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     741     927 
   Size:        741     186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     927 
   Size:        271     656
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     739     927 
   Size:        169     570     188

  Fragments arranged by size: 

                 570     188     169
 
BsaHI Gr'CG_yC

Cuts at:      0     108     138     927 
   Size:        108      30     789

  Fragments arranged by size: 

                 789     108      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     559     589     927 
   Size:        559      30     338

  Fragments arranged by size: 

                 559     338      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     457     597     927 
   Size:        457     140     330

  Fragments arranged by size: 

                 457     330     140
 
BseYI C'CCAG_C

Cuts at:      0      27      80      88      96     519     927 
   Size:         27      53       8       8     423     408

  Fragments arranged by size: 

                 423     408      53      27       8       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     927 
   Size:         43     884
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     257     383     569     683     915     927 
   Size:          7     250     126     186     114     232      12

  Fragments arranged by size: 

                 250     232     186     126     114      12       7
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     257     383     569     683     798     915     927 
   Size:          7     250     126     186     114     115     117      12

  Fragments arranged by size: 

                 250     186     126     117     115     114      12       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      51     521     927 
   Size:         51     470     406

  Fragments arranged by size: 

                 470     406      51
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     261     927 
   Size:        261     666
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      18     927 
   Size:         18     909
 
BstYI r'GATC_y

Cuts at:      0     278     857     927 
   Size:        278     579      70

  Fragments arranged by size: 

                 579     278      70
 
Bsu36I CC'TnA_GG

Cuts at:      0     580     927 
   Size:        580     347
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     226     927 
   Size:         47     179     701

  Fragments arranged by size: 

                 701     179      47
 
EcoICRI GAG'CTC

Cuts at:      0       5     381     927 
   Size:          5     376     546

  Fragments arranged by size: 

                 546     376       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     271     883     927 
   Size:        232      39     612      44

  Fragments arranged by size: 

                 612     232      44      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     148     579     927 
   Size:        148     431     348

  Fragments arranged by size: 

                 431     348     148
 
EcoO109I rG'GnC_Cy

Cuts at:      0     794     927 
   Size:        794     133
 
EcoRV GAT'ATC

Cuts at:      0     706     927 
   Size:        706     221
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     237     927 
   Size:        237     690
 
HaeII r_GCGC'y

Cuts at:      0     111     725     927 
   Size:        111     614     202

  Fragments arranged by size: 

                 614     202     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     270     302     737     769     927 
   Size:        270      32     435      32     158

  Fragments arranged by size: 

                 435     270     158      32      32
 
KasI G'GCGC_C

Cuts at:      0     107     927 
   Size:        107     820
 
MslI CAynn'nnrTG

Cuts at:      0     676     927 
   Size:        676     251
 
MspA1I CmG'CkG

Cuts at:      0      27     261     927 
   Size:         27     234     666

  Fragments arranged by size: 

                 666     234      27
 
NarI GG'CG_CC

Cuts at:      0     108     927 
   Size:        108     819
 
NdeI CA'TA_TG

Cuts at:      0     645     927 
   Size:        645     282
 
PasI CC'CwG_GG

Cuts at:      0     144     872     927 
   Size:        144     728      55

  Fragments arranged by size: 

                 728     144      55
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     834     927 
   Size:        834      93
 
PspOMI G'GGCC_C

Cuts at:      0     794     927 
   Size:        794     133
 
PstI C_TGCA'G

Cuts at:      0      48     398     927 
   Size:         48     350     529

  Fragments arranged by size: 

                 529     350      48
 
PvuII CAG'CTG

Cuts at:      0      27     261     927 
   Size:         27     234     666

  Fragments arranged by size: 

                 666     234      27
 
SacI G_AGCT'C

Cuts at:      0       7     383     927 
   Size:          7     376     544

  Fragments arranged by size: 

                 544     376       7
 
SbfI CC_TGCA'GG

Cuts at:      0      48     927 
   Size:         48     879
 
SfcI C'TryA_G

Cuts at:      0      44      62     394     927 
   Size:         44      18     332     533

  Fragments arranged by size: 

                 533     332      44      18
 
SfoI GGC'GCC

Cuts at:      0     109     927 
   Size:        109     818
 
SmaI CCC'GGG

Cuts at:      0     799     927 
   Size:        799     128
 
SmlI C'TyrA_G

Cuts at:      0     184     754     927 
   Size:        184     570     173

  Fragments arranged by size: 

                 570     184     173
 
StyI C'CwwG_G

Cuts at:      0     370     833     927 
   Size:        370     463      94

  Fragments arranged by size: 

                 463     370      94
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     559     591     652     684     927 
   Size:        559      32      61      32     243

  Fragments arranged by size: 

                 559     243      61      32      32
 
XmaI C'CCGG_G

Cuts at:      0     797     927 
   Size:        797     130
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI Bme1580I     BplI     BpmI    BpuEI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI
    BstXI    BstYI   Bsu36I     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI    HaeII    Hin4I     KasI     MslI   MspA1I     NarI
     NdeI     PasI    PflMI   PspOMI     PstI    PvuII     SacI     SbfI
     SfcI     SfoI     SmaI     SmlI     StyI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI    EcoRI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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