Restriction map for pF1KE0720
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspI        
AvaI        AviII       AvrII       BalI        BanII       
BbeI        BbsI        BcgI        BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsmBI       BsmI        BsoBI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      CpoI        
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       FbaI        
FseI        FspI        HaeII       Hin1I       HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Tru9I       Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I        416  GGTACC               G^GTACC
AccI          616  GTCGAC               GT^[AC][GT]AC
AcsI         1081  GAATTC               [GA]^AATT[CT]
AhdI          619  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          70  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1081  GAATTC               [GA]^AATT[CT]
Asp700I       198  GAATGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       416  GGTACC               G^GTACC
AspEI         619  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         235  GAGCAC               G[AT]GC[AT]^C
BamHI         795  GGATCC               G^GATCC
BbrPI         747  CACGTG               CAC^GTG
BbvCI         774  GCTGAGG              CCTCAGC(-5/-2)
BciVI         601  GGATAC               GTATCC(6/5)
BfaI          958  CTAG                 C^TAG
BsaAI         747  CACGTG               [CT]AC^GT[GA]
BsiHKAI       235  GAGCAC               G[AT]GC[AT]^C
BsiWI         671  CGTACG               C^GTACG
Bsp1407I      581  TGTACA               T^GTACA
BspDI         697  ATCGAT               AT^CGAT
BspHI         822  TCATGA               T^CATGA
BsrDI         148  GCAATG               GCAATG(2/0)
BsrGI         581  TGTACA               T^GTACA
CfoI          324  GCGC                 GCG^C
ClaI          697  ATCGAT               AT^CGAT
DraII         594  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        747  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          848  TGGCCG               [CT]^GGCC[GA]
Eam1105I      619  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          946  GAAGAG               CTCTTC(1/4)
EcoNI         786  CCTGACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      594  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1081  GAATTC               G^AATTC
EcoT22I       111  ATGCAT               ATGCA^T
HgaI          865  GACGC                GACGC(5/10)
HhaI          324  GCGC                 GCG^C
HinP1I        324  GCGC                 G^CGC
HphI         1211  TCACC                GGTGA(8/7)
KpnI          416  GGTACC               GGTAC^C
Ksp632I       946  GAAGAG               CTCTTC(1/4)
MaeI          958  CTAG                 C^TAG
MaeII         748  ACGT                 A^CGT
MaeIII        688  GTCAC                ^GT[ACGT]AC
NcoI          536  CCATGG               C^CATGG
NsiI          111  ATGCAT               ATGCA^T
PflMI        1134  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         747  CACGTG               CAC^GTG
PmlI          747  CACGTG               CAC^GTG
PvuII        1029  CAGCTG               CAG^CTG
RcaI          822  TCATGA               T^CATGA
SalI          616  GTCGAC               G^TCGAC
SspBI         581  TGTACA               T^GTACA
Tsp45I        688  GTCAC                ^GT[GC]AC
Tsp509I      1082  AATT                 ^AATT
Van91I       1134  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          957  TCTAGA               T^CTAGA
XcmI          548  CCAAGCCCTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          198  GAATGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccII         808  CGCG                 CG^CG
              867  CGCG                
AvaII         300  GGACC                G^G[AT]CC
              626  GGTCC               
BanI          416  GGTACC               G^G[CT][GA]CC
              544  GGCACC              
BsgI         1221  CTGCAC               GTGCAG(16/14)
              118  GTGCAG              
BstAPI        216  GCAGATCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              339  GCAGAATGTGC         
BstUI         808  CGCG                 CG^CG
              867  CGCG                
BstYI         795  GGATCC               [GA]^GATC[CT]
             1006  AGATCC              
DsaI          536  CCATGG               C^C[GA][CT]GG
             1227  CCGTGG              
EcoT14I       536  CCATGG               C^C[AT][AT]GG
              791  CCAAGG              
HincII         52  GTCAAC               GT[CT]^[GA]AC
              616  GTCGAC              
HindII         52  GTCAAC               GT[CT]^[GA]AC
              616  GTCGAC              
MflI          795  GGATCC               [GA]^GATC[CT]
             1006  AGATCC              
MspA1I       1029  CAGCTG               C[AC]G^C[GT]G
             1036  CCGCTG              
MvnI          808  CGCG                 CG^CG
              867  CGCG                
StyI          536  CCATGG               C^C[AT][AT]GG
              791  CCAAGG              
XhoII         795  GGATCC               [GA]^GATC[CT]
             1006  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspLU11I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MboI        MboII       MnlI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        TseI        TspRI       TthHB8I     
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