(Circular) (Six-base) MAPSORT of: pF1KE0720.seq Check: 1725 from: 1 to: 4355 pF1KE0720 4355 bp With 182 enzymes: SgfI * April 24, 2009 13:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1166 1336 1166 Size: 170 4185 AccI GT'mk_AC Cuts at: 725 1393 725 Size: 668 3687 Acc65I G'GTAC_C Cuts at: 524 1371 524 Size: 847 3508 AclI AA'CG_TT Cuts at: 4195 4195 Size: 4355 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 502 2180 2612 3359 3627 502 Size: 1678 432 747 268 1230 Fragments arranged by size: 1678 1230 747 432 268 AflIII A'CryG_T Cuts at: 392 582 821 1707 2811 3788 392 Size: 190 239 886 1104 977 959 Fragments arranged by size: 1104 977 959 886 239 190 AgeI A'CCGG_T Cuts at: 2743 2743 Size: 4355 AhdI GACnn_n'nnGTC Cuts at: 732 732 Size: 4355 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 379 411 964 996 379 Size: 32 553 32 3738 Fragments arranged by size: 3738 553 32 32 AlwNI CAG_nnn'CTG Cuts at: 183 1660 3227 183 Size: 1477 1567 1311 Fragments arranged by size: 1567 1477 1311 ApaLI G'TGCA_C Cuts at: 3125 3125 Size: 4355 ApoI r'AATT_y Cuts at: 1189 1359 3708 3964 1189 Size: 170 2349 256 1580 Fragments arranged by size: 2349 1580 256 170 AseI AT'TA_AT Cuts at: 20 20 Size: 4355 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4355 AvaI C'yCGr_G Cuts at: 1375 1375 Size: 4355 BamHI G'GATC_C Cuts at: 903 1380 903 Size: 477 3878 BanI G'GyrC_C Cuts at: 524 652 1371 1785 2033 2068 524 Size: 128 719 414 248 35 2811 Fragments arranged by size: 2811 719 414 248 128 35 BanII G_rGCy'C Cuts at: 1369 2399 1369 Size: 1030 3325 BbeI G_GCGC'C Cuts at: 1789 2037 1789 Size: 248 4107 BbvCI CC'TCA_GC Cuts at: 883 883 Size: 4355 BciVI GTATCCnnnnn_n' Cuts at: 703 2253 3014 4355 703 Size: 1550 761 1341 703 Fragments arranged by size: 1550 1341 761 703 BclI T'GATC_A Cuts at: 1875 1875 Size: 4355 BfrBI ATG'CAT Cuts at: 221 221 Size: 4355 BglII A'GATC_T Cuts at: 1870 3907 1870 Size: 2037 2318 BlpI GC'TnA_GC Cuts at: 1467 1467 Size: 4355 Bme1580I G_kGCm'C Cuts at: 950 1013 1980 2073 3129 950 Size: 63 967 93 1056 2176 Fragments arranged by size: 2176 1056 967 93 63 BmrI ACTGGGnnnn_n' Cuts at: 978 1567 1648 1983 4127 978 Size: 589 81 335 2144 1206 Fragments arranged by size: 2144 1206 589 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1231 2722 1231 Size: 1491 2864 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 361 529 656 792 1276 361 Size: 168 127 136 484 3440 Fragments arranged by size: 3440 484 168 136 127 Bpu10I CC'TnA_GC Cuts at: 883 1216 883 Size: 333 4022 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1531 2410 2902 3200 1531 Size: 879 492 298 2686 Fragments arranged by size: 2686 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4059 4059 Size: 4355 BsaAI yAC'GTr Cuts at: 857 1708 857 Size: 851 3504 BsaBI GATnn'nnATC Cuts at: 6 1869 3906 6 Size: 1863 2037 455 Fragments arranged by size: 2037 1863 455 BsaHI Gr'CG_yC Cuts at: 1786 2034 2736 4232 1786 Size: 248 702 1496 1909 Fragments arranged by size: 1909 1496 702 248 BsaWI w'CCGG_w Cuts at: 1544 2065 2743 3017 3164 3575 1544 Size: 521 678 274 147 411 2324 Fragments arranged by size: 2324 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 379 409 835 865 379 Size: 30 426 30 3869 Fragments arranged by size: 3869 426 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 400 840 1306 400 Size: 440 466 3449 Fragments arranged by size: 3449 466 440 BseYI C'CCAG_C Cuts at: 1780 2538 3115 3611 1780 Size: 758 577 496 2524 Fragments arranged by size: 2524 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 247 1314 247 Size: 1067 3288 BsiEI CG_ry'CG Cuts at: 104 1897 1943 3151 104 Size: 1793 46 1208 1308 Fragments arranged by size: 1793 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 347 1369 2147 2337 3129 347 Size: 1022 778 190 792 1573 Fragments arranged by size: 1573 1022 792 778 190 BsiWI C'GTAC_G Cuts at: 779 779 Size: 4355 Bsp1286I G_dGCh'C Cuts at: 347 950 1013 1369 1980 2073 2147 2337 2399 Size: 603 63 356 611 93 74 190 62 Cuts at: 2399 3129 347 Size: 730 1573 Fragments arranged by size: 1573 730 611 603 356 190 93 74 63 62 BspEI T'CCGG_A Cuts at: 3575 3575 Size: 4355 BspHI T'CATG_A Cuts at: 930 3531 930 Size: 2601 1754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 419 437 1166 1336 1405 1619 1921 2302 419 Size: 18 729 170 69 214 302 381 2472 Fragments arranged by size: 2472 729 381 302 214 170 69 18 BsrBI CCG'CTC Cuts at: 50 2647 2701 4185 4350 50 Size: 2597 54 1484 165 55 Fragments arranged by size: 2597 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 263 2267 263 Size: 2004 2351 BsrFI r'CCGG_y Cuts at: 2353 2534 2743 2353 Size: 181 209 3965 Fragments arranged by size: 3965 209 181 BsrGI T'GTAC_A Cuts at: 689 689 Size: 4355 BssSI C'ACGA_G Cuts at: 2626 2984 2626 Size: 358 3997 BstAPI GCAn_nnn'nTGC Cuts at: 330 453 1732 330 Size: 123 1279 2953 Fragments arranged by size: 2953 1279 123 BstBI TT'CG_AA Cuts at: 2716 2716 Size: 4355 BstYI r'GATC_y Cuts at: 1 903 1114 1380 1847 1870 2205 2451 3463 Size: 902 211 266 467 23 335 246 1012 Cuts at: 3463 3549 3561 3907 1 Size: 86 12 346 449 Fragments arranged by size: 1012 902 467 449 346 335 266 246 211 86 23 12 BtgI C'CryG_G Cuts at: 107 644 1335 107 Size: 537 691 3127 Fragments arranged by size: 3127 691 537 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2305 2486 2649 2305 Size: 181 163 4011 Fragments arranged by size: 4011 181 163 BtsI GCAGTG_nn' Cuts at: 1598 1598 Size: 4355 ClaI AT'CG_AT Cuts at: 806 806 Size: 4355 DraI TTT'AAA Cuts at: 1354 1354 Size: 4355 DraIII CAC_nnn'GTG Cuts at: 860 860 Size: 4355 DrdI GACnn_nn'nnGTC Cuts at: 2061 2919 2061 Size: 858 3497 EaeI y'GGCC_r Cuts at: 956 1940 2114 2505 2532 3877 956 Size: 984 174 391 27 1345 1434 Fragments arranged by size: 1434 1345 984 391 174 27 EagI C'GGCC_G Cuts at: 1940 1940 Size: 4355 EarI CTCTTCn'nnn_ Cuts at: 1049 2378 2588 1049 Size: 1329 210 2816 Fragments arranged by size: 2816 1329 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2563 2873 3019 4165 2563 Size: 310 146 1146 2753 Fragments arranged by size: 2753 1146 310 146 EcoICRI GAG'CTC Cuts at: 1367 1367 Size: 4355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 361 502 529 656 792 1276 2180 2612 3359 Size: 141 27 127 136 484 904 432 747 Cuts at: 3359 3627 361 Size: 268 1089 Fragments arranged by size: 1089 904 747 484 432 268 141 136 127 27 EcoNI CCTnn'n_nnAGG Cuts at: 898 898 Size: 4355 EcoO109I rG'GnC_Cy Cuts at: 703 1494 703 Size: 791 3564 EcoRI G'AATT_C Cuts at: 1189 1359 1189 Size: 170 4185 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 4355 FspI TGC'GCA Cuts at: 2136 2136 Size: 4355 HaeII r_GCGC'y Cuts at: 1789 2037 3059 4038 1789 Size: 248 1022 979 2106 Fragments arranged by size: 2106 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 718 750 888 920 2197 2227 2229 2259 718 Size: 32 138 32 1277 30 2 30 2814 Fragments arranged by size: 2814 1277 138 32 32 30 30 2 HincII GTy'rAC Cuts at: 162 726 1394 162 Size: 564 668 3123 Fragments arranged by size: 3123 668 564 KasI G'GCGC_C Cuts at: 1785 2033 1785 Size: 248 4107 KpnI G_GTAC'C Cuts at: 528 1375 528 Size: 847 3508 MluI A'CGCG_T Cuts at: 3788 3788 Size: 4355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3026 3210 3026 Size: 184 4171 MscI TGG'CCA Cuts at: 2116 2116 Size: 4355 MslI CAynn'nnrTG Cuts at: 397 403 581 2344 2471 397 Size: 6 178 1763 127 2281 Fragments arranged by size: 2281 1763 178 127 6 MspA1I CmG'CkG Cuts at: 1139 1146 1466 1780 2140 3153 3398 1139 Size: 7 320 314 360 1013 245 2096 Fragments arranged by size: 2096 1013 360 320 314 245 7 NaeI GCC'GGC Cuts at: 2536 2536 Size: 4355 NarI GG'CG_CC Cuts at: 1786 2034 1786 Size: 248 4107 NcoI C'CATG_G Cuts at: 107 644 107 Size: 537 3818 NgoMIV G'CCGG_C Cuts at: 2534 2534 Size: 4355 NsiI A_TGCA'T Cuts at: 223 223 Size: 4355 NspI r_CATG'y Cuts at: 396 586 825 1408 1908 2815 396 Size: 190 239 583 500 907 1936 Fragments arranged by size: 1936 907 583 500 239 190 PasI CC'CwG_GG Cuts at: 738 738 Size: 4355 PciI A'CATG_T Cuts at: 392 582 821 2811 392 Size: 190 239 1990 1936 Fragments arranged by size: 1990 1936 239 190 PflMI CCAn_nnn'nTGG Cuts at: 1248 1248 Size: 4355 PfoI T'CCnGG_A Cuts at: 314 377 1181 1292 314 Size: 63 804 111 3377 Fragments arranged by size: 3377 804 111 63 PmeI GTTT'AAAC Cuts at: 1354 1354 Size: 4355 PmlI CAC'GTG Cuts at: 857 857 Size: 4355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 379 411 3494 3526 379 Size: 32 3083 32 1208 Fragments arranged by size: 3083 1208 32 32 PstI C_TGCA'G Cuts at: 326 446 1163 1345 1402 2087 326 Size: 120 717 182 57 685 2594 Fragments arranged by size: 2594 717 685 182 120 57 PvuI CG_AT'CG Cuts at: 104 104 Size: 4355 PvuII CAG'CTG Cuts at: 1139 1780 2140 1139 Size: 641 360 3354 Fragments arranged by size: 3354 641 360 RsrII CG'GwC_CG Cuts at: 2550 2550 Size: 4355 SacI G_AGCT'C Cuts at: 1369 1369 Size: 4355 SalI G'TCGA_C Cuts at: 724 1392 724 Size: 668 3687 SapI GCTCTTCn'nnn_ Cuts at: 2378 2588 2378 Size: 210 4145 SbfI CC_TGCA'GG Cuts at: 1402 1402 Size: 4355 ScaI AGT'ACT Cuts at: 3872 3872 Size: 4355 SfcI C'TryA_G Cuts at: 33 322 442 1159 1341 1398 2083 3076 3267 Size: 289 120 717 182 57 685 993 191 Cuts at: 3267 33 Size: 1121 Fragments arranged by size: 1121 993 717 685 289 191 182 120 57 SfoI GGC'GCC Cuts at: 1787 2035 1787 Size: 248 4107 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4355 SmaI CCC'GGG Cuts at: 1377 1377 Size: 4355 SmlI C'TyrA_G Cuts at: 1510 2425 2917 3179 1510 Size: 915 492 262 2686 Fragments arranged by size: 2686 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4355 SphI G_CATG'C Cuts at: 1408 1908 1408 Size: 500 3855 StyI C'CwwG_G Cuts at: 107 644 899 1489 1843 107 Size: 537 255 590 354 2619 Fragments arranged by size: 2619 590 537 354 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 783 1336 1938 2071 2530 2739 783 Size: 553 602 133 459 209 2399 Fragments arranged by size: 2399 602 553 459 209 133 TatI w'GTAC_w Cuts at: 263 689 3870 263 Size: 426 3181 748 Fragments arranged by size: 3181 748 426 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 835 867 3872 3904 835 Size: 32 3005 32 1286 Fragments arranged by size: 3005 1286 32 32 Tth111I GACn'n_nGTC Cuts at: 2152 2152 Size: 4355 XbaI T'CTAG_A Cuts at: 1065 1386 1065 Size: 321 4034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 663 1850 663 Size: 1187 3168 XmaI C'CCGG_G Cuts at: 1375 1375 Size: 4355 XmnI GAAnn'nnTTC Cuts at: 310 310 Size: 4355 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AleI AlfI ApaI AscI AvrII BaeI BbsI BcgI BglI BmgBI BmtI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI