Restriction map for
pF1KE0716
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 448 GAATTT [GA]^AATT[CT]
AgeI 855 ACCGGT A^CCGGT
ApoI 448 GAATTT [GA]^AATT[CT]
Asp700I 933 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 358 GAGCTC G[AT]GC[AT]^C
BbvCI 964 CCTCAGC CCTCAGC(-5/-2)
BglII 698 AGATCT A^GATCT
BsaWI 855 ACCGGT [AT]^CCGG[AT]
BsiEI 817 CGGCCG CG[GA][CT]^CG
BsiHKAI 358 GAGCTC G[AT]GC[AT]^C
BsmI 738 GAATGC GAATGC(1/-1)
Bsp1407I 413 TGTACA T^GTACA
BspMI 494 ACCTGC ACCTGC(4/8)
BsrDI 692 GCAATG GCAATG(2/0)
BsrGI 413 TGTACA T^GTACA
BstBI 676 TTCGAA TT^CGAA
BstEII 618 GGTAACC G^GT[ACGT]ACC
BstF5I 177 CATCC GGATG(2/0)
BstPI 618 GGTAACC G^GT[ACGT]ACC
BstYI 698 AGATCT [GA]^GATC[CT]
DpnII 699 GATC ^GATC
DsaI 868 CCACGG C^C[GA][CT]GG
EagI 817 CGGCCG C^GGCCG
EarI 715 GAAGAG CTCTTC(1/4)
EclXI 817 CGGCCG C^GGCCG
Eco52I 817 CGGCCG C^GGCCG
EcoNI 711 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 618 GGTAACC G^GT[ACGT]ACC
FokI 177 CATCC GGATG(9/13)
HinfI 826 GACTC G^A[ACGT]TC
Ksp632I 715 GAAGAG CTCTTC(1/4)
MaeII 52 ACGT A^CGT
MboI 699 GATC ^GATC
MflI 698 AGATCT [GA]^GATC[CT]
MslI 852 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 699 GATC ^GATC
NspI 1146 GCATGT [GA]CATG^[CT]
NspV 676 TTCGAA TT^CGAA
PinAI 855 ACCGGT A^CCGGT
PleI 826 GACTC GAGTC(4/5)
SacI 358 GAGCTC GAGCT^C
Sau3AI 699 GATC ^GATC
SfuI 676 TTCGAA TT^CGAA
SgrAI 854 CACCGGTG C[GA]^CCGG[CT]G
SspBI 413 TGTACA T^GTACA
XcmI 323 CCATCTGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 698 AGATCT [GA]^GATC[CT]
XmnI 933 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 304 CGCG CG^CG
783 CGCG
AcyI 68 GGCGCC G[GA]^CG[CT]C
79 GGCGCC
ApaI 135 GGGCCC GGGCC^C
550 GGGCCC
BbeI 68 GGCGCC GGCGC^C
79 GGCGCC
BglI 71 GCCGCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1155 GCCAGCCGGGC
BsaHI 68 GGCGCC G[GA]^CG[CT]C
79 GGCGCC
BsgI 743 CTGCAC GTGCAG(16/14)
316 GTGCAG
BsmBI 651 CGTCTC CGTCTC(1/5)
5 GAGACG
BstUI 304 CGCG CG^CG
783 CGCG
HgaI 650 GCGTC GACGC(5/10)
784 GCGTC
Hin1I 68 GGCGCC G[GA]^CG[CT]C
79 GGCGCC
HphI 1093 GGTGA GGTGA(8/7)
844 TCACC
KasI 68 GGCGCC G^GCGCC
79 GGCGCC
MvnI 304 CGCG CG^CG
783 CGCG
NaeI 211 GCCGGC GCC^GGC
386 GCCGGC
NarI 68 GGCGCC GG^CGCC
79 GGCGCC
NgoMIV 211 GCCGGC G^CCGGC
386 GCCGGC
PstI 127 CTGCAG CTGCA^G
293 CTGCAG
PvuII 568 CAGCTG CAG^CTG
1118 CAGCTG
SfaNI 910 GATGC GCATC(5/9)
947 GCATC
SfoI 68 GGCGCC GGC^GCC
79 GGCGCC
Tsp509I 410 AATT ^AATT
449 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
RsaI Sau96I ScrFI SfcI SmaI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI