Restriction map for pF1KE0716
 (Linear) (Six-base) MAPSORT of: KIEE0716.seq  Check: 6486  from: 1  to: 1236



KIEE0716.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     503    1236 
   Size:        503     733
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22     924    1236 
   Size:         22     902     312

  Fragments arranged by size: 

                 902     312      22
 
AgeI A'CCGG_T

Cuts at:      0     855    1236 
   Size:        855     381
 
AleI CACnn'nnGTG

Cuts at:      0     856    1236 
   Size:        856     380
 
AlwNI CAG_nnn'CTG

Cuts at:      0     191     972    1198    1236 
   Size:        191     781     226      38

  Fragments arranged by size: 

                 781     226     191      38
 
ApaI G_GGCC'C

Cuts at:      0     139     554    1236 
   Size:        139     415     682

  Fragments arranged by size: 

                 682     415     139
 
ApoI r'AATT_y

Cuts at:      0     448    1236 
   Size:        448     788
 
AvaI C'yCGr_G

Cuts at:      0     233     343     363     553     875    1042    1088    1236 
   Size:        233     110      20     190     322     167      46     148

  Fragments arranged by size: 

                 322     233     190     167     148     110      46      20
            
 
BanI G'GyrC_C

Cuts at:      0      68      79     488     858    1236 
   Size:         68      11     409     370     378

  Fragments arranged by size: 

                 409     378     370      68      11
 
BanII G_rGCy'C

Cuts at:      0     139     362     554    1043    1088    1236 
   Size:        139     223     192     489      45     148

  Fragments arranged by size: 

                 489     223     192     148     139      45
 
BbeI G_GCGC'C

Cuts at:      0      72      83    1236 
   Size:         72      11    1153

  Fragments arranged by size: 

                1153      72      11
 
BbvCI CC'TCA_GC

Cuts at:      0     965    1236 
   Size:        965     271
 
BglI GCCn_nnn'nGGC

Cuts at:      0      77    1161    1236 
   Size:         77    1084      75

  Fragments arranged by size: 

                1084      77      75
 
BglII A'GATC_T

Cuts at:      0     698    1236 
   Size:        698     538
 
Bme1580I G_kGCm'C

Cuts at:      0     139     534     554     905    1236 
   Size:        139     395      20     351     331

  Fragments arranged by size: 

                 395     351     331     139      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     321    1236 
   Size:        321     915
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     741    1236 
   Size:        741     495
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     277     556     677    1126    1236 
   Size:        142     135     279     121     449     110

  Fragments arranged by size: 

                 449     279     142     135     121     110
 
Bpu10I CC'TnA_GC

Cuts at:      0     965    1236 
   Size:        965     271
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253     459     997    1236 
   Size:        253     206     538     239

  Fragments arranged by size: 

                 538     253     239     206
 
BsaHI Gr'CG_yC

Cuts at:      0      69      80    1236 
   Size:         69      11    1156

  Fragments arranged by size: 

                1156      69      11
 
BsaWI w'CCGG_w

Cuts at:      0     855    1236 
   Size:        855     381
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     259     289     877     907    1236 
   Size:        259      30     588      30     329

  Fragments arranged by size: 

                 588     329     259      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     953    1015    1119    1236 
   Size:        953      62     104     117

  Fragments arranged by size: 

                 953     117     104      62
 
BseYI C'CCAG_C

Cuts at:      0     520     566    1236 
   Size:        520      46     670

  Fragments arranged by size: 

                 670     520      46
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     728    1236 
   Size:        337     391     508

  Fragments arranged by size: 

                 508     391     337
 
BsiEI CG_ry'CG

Cuts at:      0     820    1236 
   Size:        820     416
 
BsiHKAI G_wGCw'C

Cuts at:      0     362    1236 
   Size:        362     874
 
BsmI GAATG_Cn'

Cuts at:      0     744    1236 
   Size:        744     492
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     657    1236 
   Size:        657     579
 
Bsp1286I G_dGCh'C

Cuts at:      0     139     362     534     554     905    1043    1088    1236 
   Size:        139     223     172      20     351     138      45     148

  Fragments arranged by size: 

                 351     223     172     148     139     138      45      20
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     503    1236 
   Size:        503     733
 
BsrDI GCAATG_nn'

Cuts at:      0     699    1236 
   Size:        699     537
 
BsrFI r'CCGG_y

Cuts at:      0     211     386     855    1236 
   Size:        211     175     469     381

  Fragments arranged by size: 

                 469     381     211     175
 
BsrGI T'GTAC_A

Cuts at:      0     413    1236 
   Size:        413     823
 
BstBI TT'CG_AA

Cuts at:      0     677    1236 
   Size:        677     559
 
BstEII G'GTnAC_C

Cuts at:      0     618    1236 
   Size:        618     618
 
BstYI r'GATC_y

Cuts at:      0     698    1236 
   Size:        698     538
 
BtgI C'CryG_G

Cuts at:      0     868    1236 
   Size:        868     368
 
BtsI GCAGTG_nn'

Cuts at:      0      20     829    1236 
   Size:         20     809     407

  Fragments arranged by size: 

                 809     407      20
 
EaeI y'GGCC_r

Cuts at:      0     203     817     974    1236 
   Size:        203     614     157     262

  Fragments arranged by size: 

                 614     262     203     157
 
EagI C'GGCC_G

Cuts at:      0     817    1236 
   Size:        817     419
 
EarI CTCTTCn'nnn_

Cuts at:      0     710    1236 
   Size:        710     526
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     169     230    1236 
   Size:        169      61    1006

  Fragments arranged by size: 

                1006     169      61
 
EcoICRI GAG'CTC

Cuts at:      0     360    1236 
   Size:        360     876
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22     142     277     556     677     924    1126    1236 
   Size:         22     120     135     279     121     247     202     110

  Fragments arranged by size: 

                 279     247     202     135     121     120     110      22
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     715    1236 
   Size:        715     521
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135     503     563    1128    1236 
   Size:        135     368      60     565     108

  Fragments arranged by size: 

                 565     368     135     108      60
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     639    1236 
   Size:        639     597
 
HaeII r_GCGC'y

Cuts at:      0      72      83     273     603    1236 
   Size:         72      11     190     330     633

  Fragments arranged by size: 

                 633     330     190      72      11
 
KasI G'GCGC_C

Cuts at:      0      68      79    1236 
   Size:         68      11    1157

  Fragments arranged by size: 

                1157      68      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1236 
   Size:         80    1156
 
MslI CAynn'nnrTG

Cuts at:      0     856    1236 
   Size:        856     380
 
MspA1I CmG'CkG

Cuts at:      0     298     570    1120    1236 
   Size:        298     272     550     116

  Fragments arranged by size: 

                 550     298     272     116
 
NaeI GCC'GGC

Cuts at:      0     213     388    1236 
   Size:        213     175     848

  Fragments arranged by size: 

                 848     213     175
 
NarI GG'CG_CC

Cuts at:      0      69      80    1236 
   Size:         69      11    1156

  Fragments arranged by size: 

                1156      69      11
 
NgoMIV G'CCGG_C

Cuts at:      0     211     386    1236 
   Size:        211     175     850

  Fragments arranged by size: 

                 850     211     175
 
NspI r_CATG'y

Cuts at:      0    1150    1236 
   Size:       1150      86
 
PfoI T'CCnGG_A

Cuts at:      0     254    1236 
   Size:        254     982
 
PpuMI rG'GwC_Cy

Cuts at:      0     503     563    1128    1236 
   Size:        503      60     565     108

  Fragments arranged by size: 

                 565     503     108      60
 
PspOMI G'GGCC_C

Cuts at:      0     135     550    1236 
   Size:        135     415     686

  Fragments arranged by size: 

                 686     415     135
 
PstI C_TGCA'G

Cuts at:      0     131     297    1236 
   Size:        131     166     939

  Fragments arranged by size: 

                 939     166     131
 
PvuII CAG'CTG

Cuts at:      0     570    1120    1236 
   Size:        570     550     116

  Fragments arranged by size: 

                 570     550     116
 
SacI G_AGCT'C

Cuts at:      0     362    1236 
   Size:        362     874
 
SanDI GG'GwC_CC

Cuts at:      0     563    1128    1236 
   Size:        563     565     108

  Fragments arranged by size: 

                 565     563     108
 
SfcI C'TryA_G

Cuts at:      0     127     293     470     585    1236 
   Size:        127     166     177     115     651

  Fragments arranged by size: 

                 651     177     166     127     115
 
SfoI GGC'GCC

Cuts at:      0      70      81    1236 
   Size:         70      11    1155

  Fragments arranged by size: 

                1155      70      11
 
SgrAI Cr'CCGG_yG

Cuts at:      0     855    1236 
   Size:        855     381
 
SmaI CCC'GGG

Cuts at:      0     235     345    1090    1236 
   Size:        235     110     745     146

  Fragments arranged by size: 

                 745     235     146     110
 
StyI C'CwwG_G

Cuts at:      0     110     263     926    1236 
   Size:        110     153     663     310

  Fragments arranged by size: 

                 663     310     153     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     303     861    1236 
   Size:        303     558     375

  Fragments arranged by size: 

                 558     375     303
 
TatI w'GTAC_w

Cuts at:      0     413    1215    1236 
   Size:        413     802      21

  Fragments arranged by size: 

                 802     413      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     330    1236 
   Size:        330     906
 
XmaI C'CCGG_G

Cuts at:      0     233     343    1088    1236 
   Size:        233     110     745     148

  Fragments arranged by size: 

                 745     233     148     110
 
XmnI GAAnn'nnTTC

Cuts at:      0     937    1236 
   Size:        937     299
 

 Enzymes that do cut:

     AarI     AcuI     AgeI     AleI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbeI    BbvCI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrDI    BsrFI    BsrGI    BstBI   BstEII    BstYI     BtgI     BtsI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII     KasI     MmeI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI
    SanDI     SfcI     SfoI    SgrAI     SmaI     StyI    TaqII     TatI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI    BpuEI    BsaAI    BsaBI    BsiWI    BspEI    BspHI    BsrBI
   BssHII    BssSI   BstAPI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     ZraI
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