Restriction map for pF1KE0712
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         492  GACGTC               GACGT^C
AfaI          882  GTAC                 GT^AC
AflIII        345  ACATGT               A^C[GA][CT]GT
AlwI          953  GGATC                GGATC(4/5)
ApaI          874  GGGCCC               GGGCC^C
Asp700I       150  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          604  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          165  CTCGGG               C^[CT]CG[GA]G
BbsI          349  GTCTTC               GAAGAC(2/6)
BfaI          362  CTAG                 C^TAG
BpuAI         349  GTCTTC               GAAGAC(2/6)
BsaI          616  GAGACC               GGTCTC(1/5)
BsaWI          30  ACCGGA               [AT]^CCGG[AT]
BsgI          328  CTGCAC               GTGCAG(16/14)
BsiEI        1005  CGACCG               CG[GA][CT]^CG
BsmBI          66  CGTCTC               CGTCTC(1/5)
BsoBI         165  CTCGGG               C^[CT]CG[GA]G
BspLU11I      345  ACATGT               A^CATGT
BsrDI         477  GCAATG               GCAATG(2/0)
EaeI           41  CGGCCA               [CT]^GGCC[GA]
EarI          825  GAAGAG               CTCTTC(1/4)
HincII       1213  GTCGAC               GT[CT]^[GA]AC
HindII       1213  GTCGAC               GT[CT]^[GA]AC
Ksp632I       825  GAAGAG               CTCTTC(1/4)
MaeI          362  CTAG                 C^TAG
NheI          361  GCTAGC               G^CTAGC
NspI          345  ACATGT               [GA]CATG^[CT]
PflFI         604  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         577  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          937  CTGCAG               CTGCA^G
RsaI          882  GTAC                 GT^AC
SacI          175  GAGCTC               GAGCT^C
SalI         1213  GTCGAC               G^TCGAC
SfcI          937  CTGCAG               C^T[GA][CT]AG
TaqI         1214  TCGA                 T^CGA
TfiI           98  GATTC                G^A[AT]TC
Tsp45I        764  GTGAC                ^GT[GC]AC
Tsp509I      1030  AATT                 ^AATT
Tth111I       604  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1214  TCGA                 T^CGA
Van91I        577  CCACCCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          150  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          610  GTCTAC               GT^[AC][GT]AC
             1213  GTCGAC              
AccII          39  CGCG                 CG^CG
             1177  CGCG                
Aor51HI       104  AGCGCT               AGC^GCT
              309  AGCGCT              
AspHI         175  GAGCTC               G[AT]GC[AT]^C
              453  GTGCTC              
BanI          631  GGCACC               G^G[CT][GA]CC
             1206  GGTGCC              
BcnI         1008  CCGGG                CC^[GC]GG
             1088  CCGGG               
BglII         464  AGATCT               A^GATCT
             1130  AGATCT              
BsiHKAI       175  GAGCTC               G[AT]GC[AT]^C
              453  GTGCTC              
BsmAI         616  GAGAC                GTCTC(1/5)
               67  GTCTC               
BsrBI         654  CCGCTC               CCGCTC(-3/-3)
              703  GAGCGG              
BstUI          39  CGCG                 CG^CG
             1177  CGCG                
BstYI         464  AGATCT               [GA]^GATC[CT]
             1130  AGATCT              
Eco47III      104  AGCGCT               AGC^GCT
              309  AGCGCT              
EcoNI         248  CCTCGGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              858  CCTGCGGAAGG         
FauI          705  GCGGG                CCCGC(4/6)
             1197  GCGGG               
HphI          763  GGTGA                GGTGA(8/7)
             1044  GGTGA               
MaeII         493  ACGT                 A^CGT
              890  ACGT                
MaeIII        764  GTGAC                ^GT[ACGT]AC
             1106  GTTAC               
MflI          464  AGATCT               [GA]^GATC[CT]
             1130  AGATCT              
MslI          189  CACCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              957  CATGCTGGTG          
MvnI           39  CGCG                 CG^CG
             1177  CGCG                
NciI         1008  CCGGG                CC^[GC]GG
             1088  CCGGG               
XhoII         464  AGATCT               [GA]^GATC[CT]
             1130  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcyI        AluI        AvaII       BanII       
BbvI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        TseI        
TspRI       
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