Restriction map for pF1KE0712
 (Linear) (Six-base) MAPSORT of: KIEE0712.seq  Check: 8164  from: 1  to: 1233



KIEE0712.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AatII G_ACGT'C

Cuts at:      0     496    1233 
   Size:        496     737
 
AccI GT'mk_AC

Cuts at:      0     611    1214    1233 
   Size:        611     603      19

  Fragments arranged by size: 

                 611     603      19
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     501    1233 
   Size:        501     732
 
AfeI AGC'GCT

Cuts at:      0     106     311    1233 
   Size:        106     205     922

  Fragments arranged by size: 

                 922     205     106
 
AflIII A'CryG_T

Cuts at:      0     345    1233 
   Size:        345     888
 
AleI CACnn'nnGTG

Cuts at:      0     193    1233 
   Size:        193    1040
 
ApaI G_GGCC'C

Cuts at:      0     878    1233 
   Size:        878     355
 
AvaI C'yCGr_G

Cuts at:      0     165    1233 
   Size:        165    1068
 
BanI G'GyrC_C

Cuts at:      0     631    1206    1233 
   Size:        631     575      27

  Fragments arranged by size: 

                 631     575      27
 
BanII G_rGCy'C

Cuts at:      0     172     179     878    1233 
   Size:        172       7     699     355

  Fragments arranged by size: 

                 699     355     172       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     342    1233 
   Size:        342     891
 
BglII A'GATC_T

Cuts at:      0     464    1130    1233 
   Size:        464     666     103

  Fragments arranged by size: 

                 666     464     103
 
Bme1580I G_kGCm'C

Cuts at:      0     187     878    1233 
   Size:        187     691     355

  Fragments arranged by size: 

                 691     355     187
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     283     593    1233 
   Size:        283     310     640

  Fragments arranged by size: 

                 640     310     283
 
BmtI G_CTAG'C

Cuts at:      0     365    1233 
   Size:        365     868
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     483    1233 
   Size:        483     750
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61      76     642     761     965    1233 
   Size:         61      15     566     119     204     268

  Fragments arranged by size: 

                 566     268     204     119      61      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     163    1102    1233 
   Size:        103      60     939     131

  Fragments arranged by size: 

                 939     131     103      60
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     610    1233 
   Size:        610     623
 
BsaHI Gr'CG_yC

Cuts at:      0     205     493    1217    1233 
   Size:        205     288     724      16

  Fragments arranged by size: 

                 724     288     205      16
 
BsaWI w'CCGG_w

Cuts at:      0      30    1233 
   Size:         30    1203
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     684     714     827     857    1233 
   Size:        684      30     113      30     376

  Fragments arranged by size: 

                 684     376     113      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475     766     835    1233 
   Size:        475     291      69     398

  Fragments arranged by size: 

                 475     398     291      69
 
BseYI C'CCAG_C

Cuts at:      0     792    1023    1233 
   Size:        792     231     210

  Fragments arranged by size: 

                 792     231     210
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313    1233 
   Size:        313     920
 
BsiEI CG_ry'CG

Cuts at:      0    1008    1233 
   Size:       1008     225
 
BsiHKAI G_wGCw'C

Cuts at:      0     179     457    1233 
   Size:        179     278     776

  Fragments arranged by size: 

                 776     278     179
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      72    1233 
   Size:         72    1161
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     179     187     457     878    1233 
   Size:        172       7       8     270     421     355

  Fragments arranged by size: 

                 421     355     270     172       8       7
 
BsrBI CCG'CTC

Cuts at:      0     656     705    1233 
   Size:        656      49     528

  Fragments arranged by size: 

                 656     528      49
 
BsrDI GCAATG_nn'

Cuts at:      0     484    1233 
   Size:        484     749
 
BstYI r'GATC_y

Cuts at:      0     464    1130    1233 
   Size:        464     666     103

  Fragments arranged by size: 

                 666     464     103
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     432    1233 
   Size:        432     801
 
BtsI GCAGTG_nn'

Cuts at:      0     217    1191    1233 
   Size:        217     974      42

  Fragments arranged by size: 

                 974     217      42
 
EaeI y'GGCC_r

Cuts at:      0      41    1233 
   Size:         41    1192
 
EarI CTCTTCn'nnn_

Cuts at:      0     820    1233 
   Size:        820     413
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      60     496    1233 
   Size:         60     436     737

  Fragments arranged by size: 

                 737     436      60
 
EcoICRI GAG'CTC

Cuts at:      0     177    1233 
   Size:        177    1056
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61      76     501     642     761     965    1233 
   Size:         61      15     425     141     119     204     268

  Fragments arranged by size: 

                 425     268     204     141     119      61      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     252     862    1233 
   Size:        252     610     371

  Fragments arranged by size: 

                 610     371     252
 
EcoO109I rG'GnC_Cy

Cuts at:      0     258     757     874    1200    1233 
   Size:        258     499     117     326      33

  Fragments arranged by size: 

                 499     326     258     117      33
 
HaeII r_GCGC'y

Cuts at:      0     108     313    1071    1233 
   Size:        108     205     758     162

  Fragments arranged by size: 

                 758     205     162     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     757     789     827     859     938     970    1233 
   Size:        757      32      38      32      79      32     263

  Fragments arranged by size: 

                 757     263      79      38      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1215    1233 
   Size:       1215      18
 
MslI CAynn'nnrTG

Cuts at:      0     193     961    1233 
   Size:        193     768     272

  Fragments arranged by size: 

                 768     272     193
 
NheI G'CTAG_C

Cuts at:      0     361    1233 
   Size:        361     872
 
NspI r_CATG'y

Cuts at:      0     349    1233 
   Size:        349     884
 
PasI CC'CwG_GG

Cuts at:      0      58    1233 
   Size:         58    1175
 
PciI A'CATG_T

Cuts at:      0     345    1233 
   Size:        345     888
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     583    1233 
   Size:        583     650
 
PfoI T'CCnGG_A

Cuts at:      0      77     338     831    1087    1233 
   Size:         77     261     493     256     146

  Fragments arranged by size: 

                 493     261     256     146      77
 
PspOMI G'GGCC_C

Cuts at:      0     874    1233 
   Size:        874     359
 
PstI C_TGCA'G

Cuts at:      0     941    1233 
   Size:        941     292
 
SacI G_AGCT'C

Cuts at:      0     179    1233 
   Size:        179    1054
 
SalI G'TCGA_C

Cuts at:      0    1213    1233 
   Size:       1213      20
 
SfcI C'TryA_G

Cuts at:      0     937    1233 
   Size:        937     296
 
SmlI C'TyrA_G

Cuts at:      0     118     178    1117    1233 
   Size:        118      60     939     116

  Fragments arranged by size: 

                 939     118     116      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19      61     255     594     733    1233 
   Size:         19      42     194     339     139     500

  Fragments arranged by size: 

                 500     339     194     139      42      19
 
TatI w'GTAC_w

Cuts at:      0     881    1233 
   Size:        881     352
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     684     716    1233 
   Size:        684      32     517

  Fragments arranged by size: 

                 684     517      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     607    1233 
   Size:        607     626
 
XmnI GAAnn'nnTTC

Cuts at:      0     154    1233 
   Size:        154    1079
 
ZraI GAC'GTC

Cuts at:      0     494    1233 
   Size:        494     739
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI   AflIII     AleI     ApaI     AvaI
     BanI    BanII     BbsI    BglII Bme1580I     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrDI    BstYI
    BtgZI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     MslI     NheI     NspI     PasI
     PciI    PflMI     PfoI   PspOMI     PstI     SacI     SalI     SfcI
     SmlI    TaqII     TatI     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI   Bpu10I    BsaAI    BsaBI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     XbaI     XcmI     XhoI     XmaI
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