Restriction map for
pF1KE0708
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HapII Hin1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NcoI NgoMIV NheI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI TaqI TseI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 12 CGCG CG^CG
AflIII 805 ACGTGT A^C[GA][CT]GT
AlwNI 346 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 418 GAGCAC G[AT]GC[AT]^C
BanI 189 GGCACC G^G[CT][GA]CC
BsaAI 804 TACGTG [CT]AC^GT[GA]
BseRI 454 GAGGAG GAGGAG(10/8)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiHKAI 418 GAGCAC G[AT]GC[AT]^C
BsmFI 840 GTCCC GGGAC(10/14)
BsmI 973 GAATGC GAATGC(1/-1)
Bsp1407I 852 TGTACA T^GTACA
BspMI 247 GCAGGT ACCTGC(4/8)
BsrDI 933 CATTGC GCAATG(2/0)
BsrGI 852 TGTACA T^GTACA
BssSI 688 CACGAG CACGAG(-5/-1)
BstAPI 345 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 646 GGATG GGATG(2/0)
BstUI 12 CGCG CG^CG
CfoI 13 GCGC GCG^C
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoRI 863 GAATTC G^AATTC
EcoT22I 648 ATGCAT ATGCA^T
FokI 646 GGATG GGATG(9/13)
HgaI 126 GACGC GACGC(5/10)
HhaI 13 GCGC GCG^C
HinP1I 13 GCGC G^CGC
MseI 830 TTAA T^TAA
MspA1I 636 CAGCTG C[AC]G^C[GT]G
MvnI 12 CGCG CG^CG
NotI 7 GCGGCCGC GC^GGCCGC
NsiI 648 ATGCAT ATGCA^T
PshAI 225 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 636 CAGCTG CAG^CTG
SspBI 852 TGTACA T^GTACA
StuI 365 AGGCCT AGG^CCT
TfiI 84 GAATC G^A[AT]TC
Tru9I 830 TTAA T^TAA
XcmI 630 CCATTACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BbsI 49 GAAGAC GAAGAC(2/6)
508 GTCTTC
BfaI 746 CTAG C^TAG
788 CTAG
BmyI 33 GGGCAC G[AGT]GC[ACT]^C
418 GAGCAC
BpuAI 49 GAAGAC GAAGAC(2/6)
508 GTCTTC
Bsp1286I 33 GGGCAC G[AGT]GC[ACT]^C
418 GAGCAC
Cfr13I 267 GGCCC G^G[ACGT]CC
289 GGCCC
DpnII 294 GATC ^GATC
322 GATC
EarI 1176 CTCTTC CTCTTC(1/4)
471 GAAGAG
EcoNI 90 CCTTAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
241 CCTATGGCAGG
Ksp632I 1176 CTCTTC CTCTTC(1/4)
471 GAAGAG
MaeI 746 CTAG C^TAG
788 CTAG
MaeII 53 ACGT A^CGT
805 ACGT
MaeIII 40 GTGAC ^GT[ACGT]AC
124 GTGAC
MboI 294 GATC ^GATC
322 GATC
NdeI 884 CATATG CA^TATG
968 CATATG
NdeII 294 GATC ^GATC
322 GATC
NlaIV 20 GGTTCC GG[ACGT]^[ACGT]CC
189 GGCACC
NspI 312 GCATGT [GA]CATG^[CT]
486 GCATGT
Sau3AI 294 GATC ^GATC
322 GATC
Sau96I 267 GGCCC G^G[ACGT]CC
289 GGCCC
SfaNI 107 GATGC GCATC(5/9)
647 GATGC
SfcI 438 CTACAG C^T[GA][CT]AG
704 CTACAG
Tsp45I 40 GTGAC ^GT[GC]AC
124 GTGAC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsrI BssKI BstNI DdeI
EcoRII Fnu4HI HaeIII HinfI ItaI
MboII MnlI MslI MvaI MwoI
NlaIII PleI RsaI ScrFI Tsp509I
TspRI