################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BanI BbeI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI DraI DrdI Eam1105I EarI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TaqI Tru9I Tsp45I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 36 TCCGGA T^CCGGA AcyI 10 GGCGTC G[GA]^CG[CT]C AflIII 548 ACACGT A^C[GA][CT]GT Aor51HI 632 AGCGCT AGC^GCT Asp700I 207 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 7 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 641 TGGCCA TGG^CCA BamHI 236 GGATCC G^GATCC BbrPI 549 CACGTG CAC^GTG BbsI 819 GAAGAC GAAGAC(2/6) BpuAI 819 GAAGAC GAAGAC(2/6) BsaAI 549 CACGTG [CT]AC^GT[GA] BsaHI 10 GGCGTC G[GA]^CG[CT]C BseAI 36 TCCGGA T^CCGGA BsgI 421 GTGCAG GTGCAG(16/14) BsiEI 1026 CGGCCG CG[GA][CT]^CG BsmAI 593 GTCTC GTCTC(1/5) Bsp1407I 299 TGTACA T^GTACA BspEI 36 TCCGGA T^CCGGA BspMI 674 ACCTGC ACCTGC(4/8) BsrBI 43 GAGCGG CCGCTC(-3/-3) BsrDI 196 GCAATG GCAATG(2/0) BsrGI 299 TGTACA T^GTACA BstAPI 78 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 779 CGGACCG CG^G[AT]CCG DraIII 1048 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 881 CCACGG C^C[GA][CT]GG EagI 1026 CGGCCG C^GGCCG EclXI 1026 CGGCCG C^GGCCG Eco47III 632 AGCGCT AGC^GCT Eco52I 1026 CGGCCG C^GGCCG HgaI 11 GCGTC GACGC(5/10) Hin1I 10 GGCGTC G[GA]^CG[CT]C MluNI 641 TGGCCA TGG^CCA MroI 36 TCCGGA T^CCGGA MscI 641 TGGCCA TGG^CCA PflFI 7 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 240 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 33 GACTC GAGTC(4/5) PmaCI 549 CACGTG CAC^GTG PmlI 549 CACGTG CAC^GTG PpuMI 671 GGGACCT [GA]G^G[AT]CC[CT] PstI 265 CTGCAG CTGCA^G RsrII 779 CGGACCG CG^G[AT]CCG SacI 115 GAGCTC GAGCT^C SfcI 265 CTGCAG C^T[GA][CT]AG SspBI 299 TGTACA T^GTACA StuI 494 AGGCCT AGG^CCT Tsp509I 477 AATT ^AATT Tth111I 7 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 240 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 128 CCATGAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 207 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AlwNI 79 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 100 CAGCGCCTG AspHI 115 GAGCTC G[AT]GC[AT]^C 373 GTGCTC AvaI 277 CCCGAG C^[CT]CG[GA]G 896 CCCGAG BsiHKAI 115 GAGCTC G[AT]GC[AT]^C 373 GTGCTC BsoBI 277 CCCGAG C^[CT]CG[GA]G 896 CCCGAG DraII 425 AGGCCCT [GA]G^G[ACGT]CC[CT] 671 GGGACCT EaeI 641 TGGCCA [CT]^GGCC[GA] 1026 CGGCCG EcoO109I 425 AGGCCCT [GA]G^G[ACGT]CC[CT] 671 GGGACCT HphI 816 GGTGA GGTGA(8/7) 404 TCACC MaeII 550 ACGT A^CGT 823 ACGT TspRI 120 CAGTG CA[GC]TG[ACGT][ACGT]^ 371 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AocI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsaWI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII Eco81I EcoNI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI XhoII