Restriction map for pF1KE0661
 (Circular) (Six-base) MAPSORT of: pF1KE0661.seq  Check: 9707  from: 1  to: 3821



pF1KE0661  3821 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:    859     859 
   Size:       3821
 
Acc65I G'GTAC_C

Cuts at:    837     837 
   Size:       3821
 
AclI AA'CG_TT

Cuts at:   3661    3661 
   Size:       3821
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1646    2078    2825    3093    1646 
   Size:        432     747     268    2374

  Fragments arranged by size: 

                2374     747     432     268
 
AflIII A'CryG_T

Cuts at:    513    1173    2277    3254     513 
   Size:        660    1104     977    1080

  Fragments arranged by size: 

                1104    1080     977     660
 
AgeI A'CCGG_T

Cuts at:   2209    2209 
   Size:       3821
 
AlwNI CAG_nnn'CTG

Cuts at:    532     595     598     658     754     757     795    1126    2693 
   Size:         63       3      60      96       3      38     331    1567

Cuts at:   2693     532 
   Size:       1660

  Fragments arranged by size: 

                1660    1567     331      96      63      60      38       3
                   3
 
ApaLI G'TGCA_C

Cuts at:   2591    2591 
   Size:       3821
 
ApoI r'AATT_y

Cuts at:    825    3174    3430     825 
   Size:       2349     256    1216

  Fragments arranged by size: 

                2349    1216     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3821
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3821
 
AvaI C'yCGr_G

Cuts at:    260     841     260 
   Size:        581    3240
 
BamHI G'GATC_C

Cuts at:    318     846     318 
   Size:        528    3293
 
BanI G'GyrC_C

Cuts at:    720     837    1251    1499    1534     720 
   Size:        117     414     248      35    3007

  Fragments arranged by size: 

                3007     414     248     117      35
 
BanII G_rGCy'C

Cuts at:    835    1865     835 
   Size:       1030    2791
 
BbeI G_GCGC'C

Cuts at:   1255    1503    1255 
   Size:        248    3573
 
BbsI GAAGACnn'nnnn_

Cuts at:    198     198 
   Size:       3821
 
BbvCI CC'TCA_GC

Cuts at:    788     788 
   Size:       3821
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     180     214      90 
   Size:         34      56      34    3697

  Fragments arranged by size: 

                3697      56      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:    449    1719    2480    3821     449 
   Size:       1270     761    1341     449

  Fragments arranged by size: 

                1341    1270     761     449
 
BclI T'GATC_A

Cuts at:   1341    1341 
   Size:       3821
 
BglI GCCn_nnn'nGGC

Cuts at:    307     307 
   Size:       3821
 
BglII A'GATC_T

Cuts at:    143    1336    3373     143 
   Size:       1193    2037     591

  Fragments arranged by size: 

                2037    1193     591
 
BlpI GC'TnA_GC

Cuts at:    933     933 
   Size:       3821
 
Bme1580I G_kGCm'C

Cuts at:   1446    1539    2595    1446 
   Size:         93    1056    2672

  Fragments arranged by size: 

                2672    1056      93
 
BmgBI CAC'GTC

Cuts at:    516     516 
   Size:       3821
 
BmrI ACTGGGnnnn_n'

Cuts at:   1033    1114    1449    3593    1033 
   Size:         81     335    2144    1261

  Fragments arranged by size: 

                2144    1261     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2188    2188 
   Size:       3821
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     635     587 
   Size:         48    3773
 
Bpu10I CC'TnA_GC

Cuts at:    788     788 
   Size:       3821
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    997    1876    2368    2666     997 
   Size:        879     492     298    2152

  Fragments arranged by size: 

                2152     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3525    3525 
   Size:       3821
 
BsaAI yAC'GTr

Cuts at:   1174    1174 
   Size:       3821
 
BsaBI GATnn'nnATC

Cuts at:      6     323    1335    3372       6 
   Size:        317    1012    2037     455

  Fragments arranged by size: 

                2037    1012     455     317
 
BsaHI Gr'CG_yC

Cuts at:    179    1252    1500    2202    3698     179 
   Size:       1073     248     702    1496     302

  Fragments arranged by size: 

                1496    1073     702     302     248
 
BsaWI w'CCGG_w

Cuts at:   1010    1531    2209    2483    2630    3041    1010 
   Size:        521     678     274     147     411    1790

  Fragments arranged by size: 

                1790     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    235     235 
   Size:       3821
 
BseYI C'CCAG_C

Cuts at:    676    1246    2004    2581    3077     676 
   Size:        570     758     577     496    1420

  Fragments arranged by size: 

                1420     758     577     570     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    511     511 
   Size:       3821
 
BsiEI CG_ry'CG

Cuts at:    104     243    1363    1409    2617     104 
   Size:        139    1120      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1120     139      46
 
BsiHKAI G_wGCw'C

Cuts at:    835    1613    1803    2595     835 
   Size:        778     190     792    2061

  Fragments arranged by size: 

                2061     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    815     815 
   Size:       3821
 
Bsp1286I G_dGCh'C

Cuts at:    835    1446    1539    1613    1803    1865    2595     835 
   Size:        611      93      74     190      62     730    2061

  Fragments arranged by size: 

                2061     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3041    3041 
   Size:       3821
 
BspHI T'CATG_A

Cuts at:   2997    2997 
   Size:       3821
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    468     871    1085    1387    1768     468 
   Size:        403     214     302     381    2521

  Fragments arranged by size: 

                2521     403     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2113    2167    3651    3816      50 
   Size:       2063      54    1484     165      55

  Fragments arranged by size: 

                2063    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1733    1733 
   Size:       3821
 
BsrFI r'CCGG_y

Cuts at:    242    1819    2000    2209     242 
   Size:       1577     181     209    1854

  Fragments arranged by size: 

                1854    1577     209     181
 
BssSI C'ACGA_G

Cuts at:   2092    2450    2092 
   Size:        358    3463
 
BstAPI GCAn_nnn'nTGC

Cuts at:    598     754     757    1198     598 
   Size:        156       3     441    3221

  Fragments arranged by size: 

                3221     441     156       3
 
BstBI TT'CG_AA

Cuts at:   2182    2182 
   Size:       3821
 
BstYI r'GATC_y

Cuts at:      1     143     318     846    1313    1336    1671    1917    2929 
   Size:        142     175     528     467      23     335     246    1012

Cuts at:   2929    3015    3027    3373       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     528     467     449     346     335     246     175
                 142      86      23      12
 
BtgI C'CryG_G

Cuts at:    281     281 
   Size:       3821
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1771    1952    2115    1771 
   Size:        181     163    3477

  Fragments arranged by size: 

                3477     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1064    1064 
   Size:       3821
 
DraI TTT'AAA

Cuts at:    820     820 
   Size:       3821
 
DrdI GACnn_nn'nnGTC

Cuts at:    255    1527    2385     255 
   Size:       1272     858    1691

  Fragments arranged by size: 

                1691    1272     858
 
EaeI y'GGCC_r

Cuts at:    774    1406    1580    1971    1998    3343     774 
   Size:        632     174     391      27    1345    1252

  Fragments arranged by size: 

                1345    1252     632     391     174      27
 
EagI C'GGCC_G

Cuts at:   1406    1406 
   Size:       3821
 
EarI CTCTTCn'nnn_

Cuts at:   1844    2054    1844 
   Size:        210    3611
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2029    2339    2485    3631    2029 
   Size:        310     146    1146    2219

  Fragments arranged by size: 

                2219    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    833     833 
   Size:       3821
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    587     635    1646    2078    2825    3093     587 
   Size:         48    1011     432     747     268    1315

  Fragments arranged by size: 

                1315    1011     747     432     268      48
 
EcoO109I rG'GnC_Cy

Cuts at:    308     960     308 
   Size:        652    3169
 
EcoRI G'AATT_C

Cuts at:    825     825 
   Size:       3821
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1840    1840 
   Size:       3821
 
FspI TGC'GCA

Cuts at:    190    1602     190 
   Size:       1412    2409
 
HaeII r_GCGC'y

Cuts at:   1255    1503    2525    3504    1255 
   Size:        248    1022     979    1572

  Fragments arranged by size: 

                1572    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1663    1693    1695    1725    1663 
   Size:         30       2      30    3759

  Fragments arranged by size: 

                3759      30      30       2
 
HincII GTy'rAC

Cuts at:    860     860 
   Size:       3821
 
KasI G'GCGC_C

Cuts at:   1251    1499    1251 
   Size:        248    3573
 
KpnI G_GTAC'C

Cuts at:    841     841 
   Size:       3821
 
MluI A'CGCG_T

Cuts at:   3254    3254 
   Size:       3821
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2492    2676    2492 
   Size:        184    3637
 
MscI TGG'CCA

Cuts at:    776    1582     776 
   Size:        806    3015
 
MslI CAynn'nnrTG

Cuts at:   1810    1937    1810 
   Size:        127    3694
 
MspA1I CmG'CkG

Cuts at:    532     595     616     655     718     733     745     754     763 
   Size:         63      21      39      63      15      12       9       9

Cuts at:    763     795     932    1246    1606    2619    2864     532 
   Size:         32     137     314     360    1013     245    1489

  Fragments arranged by size: 

                1489    1013     360     314     245     137      63      63
                  39      32      21      15      12       9       9
 
NaeI GCC'GGC

Cuts at:   2002    2002 
   Size:       3821
 
NarI GG'CG_CC

Cuts at:   1252    1500    1252 
   Size:        248    3573
 
NcoI C'CATG_G

Cuts at:    281     281 
   Size:       3821
 
NgoMIV G'CCGG_C

Cuts at:   2000    2000 
   Size:       3821
 
NspI r_CATG'y

Cuts at:    874    1374    2281     874 
   Size:        500     907    2414

  Fragments arranged by size: 

                2414     907     500
 
PciI A'CATG_T

Cuts at:   2277    2277 
   Size:       3821
 
PmeI GTTT'AAAC

Cuts at:    820     820 
   Size:       3821
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2960    2992    2960 
   Size:         32    3789
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    349     381     349 
   Size:         32    3789
 
PstI C_TGCA'G

Cuts at:    663     750     768     800     868    1553     663 
   Size:         87      18      32      68     685    2931

  Fragments arranged by size: 

                2931     685      87      68      32      18
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3821
 
PvuII CAG'CTG

Cuts at:    532     595     655     733     754     795    1246    1606     532 
   Size:         63      60      78      21      41     451     360    2747

  Fragments arranged by size: 

                2747     451     360      78      63      60      41      21
            
 
RsrII CG'GwC_CG

Cuts at:   2016    2016 
   Size:       3821
 
SacI G_AGCT'C

Cuts at:    835     835 
   Size:       3821
 
SalI G'TCGA_C

Cuts at:    858     858 
   Size:       3821
 
SapI GCTCTTCn'nnn_

Cuts at:   1844    2054    1844 
   Size:        210    3611
 
SbfI CC_TGCA'GG

Cuts at:    868     868 
   Size:       3821
 
ScaI AGT'ACT

Cuts at:    196    3338     196 
   Size:       3142     679
 
SexAI A'CCwGG_T

Cuts at:    396     396 
   Size:       3821
 
SfcI C'TryA_G

Cuts at:     33     659     746     764     796     864    1549    2542    2733 
   Size:        626      87      18      32      68     685     993     191

Cuts at:   2733      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     626     191      87      68      32
                  18
 
SfoI GGC'GCC

Cuts at:   1253    1501    1253 
   Size:        248    3573
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3821
 
SmaI CCC'GGG

Cuts at:    262     843     262 
   Size:        581    3240
 
SmlI C'TyrA_G

Cuts at:    976    1891    2383    2645     976 
   Size:        915     492     262    2152

  Fragments arranged by size: 

                2152     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3821
 
SphI G_CATG'C

Cuts at:    874    1374     874 
   Size:        500    3321
 
StyI C'CwwG_G

Cuts at:    281     382     955    1309     281 
   Size:        101     573     354    2793

  Fragments arranged by size: 

                2793     573     354     101
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    821    1404    1537    1996    2205     821 
   Size:        583     133     459     209    2437

  Fragments arranged by size: 

                2437     583     459     209     133
 
TatI w'GTAC_w

Cuts at:    194    3336     194 
   Size:       3142     679
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3338    3370    3338 
   Size:         32    3789
 
Tth111I GACn'n_nGTC

Cuts at:   1618    1618 
   Size:       3821
 
XbaI T'CTAG_A

Cuts at:    852     852 
   Size:       3821
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    357    1316     357 
   Size:        959    2862
 
XmaI C'CCGG_G

Cuts at:    260     841     260 
   Size:        581    3240
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BfrBI     BmtI    BsaXI    BsiWI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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