Restriction map for pF1KE0645
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmFI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SapI        SbfI        SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StyI        SwaI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          394  GTCGAC               GT^[AC][GT]AC
Alw44I        643  GTGCAC               G^TGCAC
ApaLI         643  GTGCAC               G^TGCAC
BglII         434  AGATCT               A^GATCT
BlpI          528  GCTCAGC              GC^T[ACGT]AGC
BpmI          562  CTGGAG               CTGGAG(16/14)
Bpu1102I      528  GCTCAGC              GC^T[ACGT]AGC
BsaAI         631  TACGTG               [CT]AC^GT[GA]
BsaI          305  GAGACC               GGTCTC(1/5)
Bsp1407I       17  TGTACA               T^GTACA
BsrFI         593  GCCGGT               [GA]^CCGG[CT]
BsrGI          17  TGTACA               T^GTACA
BstAPI        636  GCAGCCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         528  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        593  GCCGGT               [GA]^CCGG[CT]
EaeI          206  TGGCCG               [CT]^GGCC[GA]
EarI          211  GAAGAG               CTCTTC(1/4)
EcoNI         357  CCTGTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         566  AGCGCC               [GA]GCGC^[CT]
HgaI          161  GCGTC                GACGC(5/10)
HincII        394  GTCGAC               GT[CT]^[GA]AC
HindII        394  GTCGAC               GT[CT]^[GA]AC
HphI          469  TCACC                GGTGA(8/7)
Ksp632I       211  GAAGAG               CTCTTC(1/4)
MaeII         632  ACGT                 A^CGT
MspA1I        202  CAGCTG               C[AC]G^C[GT]G
NlaIII         87  CATG                 CATG^
PleI            4  GAGTC                GAGTC(4/5)
PvuII         202  CAGCTG               CAG^CTG
SalI          394  GTCGAC               G^TCGAC
ScaI          350  AGTACT               AGT^ACT
SexAI         308  ACCAGGT              A^CC[AT]GGT
SfcI           32  CTACAG               C^T[GA][CT]AG
SspBI          17  TGTACA               T^GTACA
StuI          338  AGGCCT               AGG^CCT
TfiI          216  GATTC                G^A[AT]TC
Tsp45I        468  GTCAC                ^GT[GC]AC
TspRI         580  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          571  CCAGACCCCCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI           78  GATCC                GGATC(4/5)
              252  GATCC               
Asp700I       211  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              348  GAAGTACTTC          
AvaI          183  CTCGGG               C^[CT]CG[GA]G
              342  CTCGGG              
BseRI         364  GAGGAG               GAGGAG(10/8)
              451  GAGGAG              
BsgI          313  GTGCAG               GTGCAG(16/14)
              634  GTGCAG              
BsmAI          65  GAGAC                GTCTC(1/5)
              305  GAGAC               
BsoBI         183  CTCGGG               C^[CT]CG[GA]G
              342  CTCGGG              
BstYI          77  AGATCC               [GA]^GATC[CT]
              434  AGATCT              
DdeI          507  CTCAG                C^T[ACGT]AG
              529  CTCAG               
FauI          588  GCGGG                CCCGC(4/6)
              602  GCGGG               
HinfI           4  GAGTC                G^A[ACGT]TC
              216  GATTC               
MaeIII        468  GTCAC                ^GT[ACGT]AC
              629  GTTAC               
MflI           77  AGATCC               [GA]^GATC[CT]
              434  AGATCT              
PpuMI         515  AGGACCC              [GA]G^G[AT]CC[CT]
              557  AGGTCCT             
SacI          118  GAGCTC               GAGCT^C
              267  GAGCTC              
SmlI           73  CTCAAG               C^T[CT][GA]AG
              463  CTCAAG              
TaqI          131  TCGA                 T^CGA
              395  TCGA                
TthHB8I       131  TCGA                 T^CGA
              395  TCGA                
XhoII          77  AGATCC               [GA]^GATC[CT]
              434  AGATCT              
XmnI          211  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              348  GAAGTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       DraII       
DsaI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MboI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com