Restriction map for pF1KE0645
 (Linear) (Six-base) MAPSORT of: KIEE0645.seq  Check: 3651  from: 1  to: 651



KIEE0645.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0     395     651 
   Size:        395     256
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     355     387     651 
   Size:        355      32     264

  Fragments arranged by size: 

                 355     264      32
 
ApaLI G'TGCA_C

Cuts at:      0     643     651 
   Size:        643       8
 
AvaI C'yCGr_G

Cuts at:      0     183     342     651 
   Size:        183     159     309

  Fragments arranged by size: 

                 309     183     159
 
BanII G_rGCy'C

Cuts at:      0     122     271     458     651 
   Size:        122     149     187     193

  Fragments arranged by size: 

                 193     187     149     122
 
BglII A'GATC_T

Cuts at:      0     434     651 
   Size:        434     217
 
BlpI GC'TnA_GC

Cuts at:      0     529     651 
   Size:        529     122
 
Bme1580I G_kGCm'C

Cuts at:      0     647     651 
   Size:        647       4
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     489     651 
   Size:        489     162
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      88     651 
   Size:         88     563
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     651 
   Size:        583      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     448     651 
   Size:         58     390     203

  Fragments arranged by size: 

                 390     203      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     299     651 
   Size:        299     352
 
BsaAI yAC'GTr

Cuts at:      0     633     651 
   Size:        633      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     296     326     355     385     651 
   Size:        296      30      29      30     266

  Fragments arranged by size: 

                 296     266      30      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     379     466     651 
   Size:        379      87     185

  Fragments arranged by size: 

                 379     185      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     651 
   Size:        334     317
 
BsiHKAI G_wGCw'C

Cuts at:      0     122     271     647     651 
   Size:        122     149     376       4

  Fragments arranged by size: 

                 376     149     122       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     271     458     647     651 
   Size:        122     149     187     189       4

  Fragments arranged by size: 

                 189     187     149     122       4
 
BsrFI r'CCGG_y

Cuts at:      0     593     651 
   Size:        593      58
 
BsrGI T'GTAC_A

Cuts at:      0      17     651 
   Size:         17     634
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     642     651 
   Size:        642       9
 
BstYI r'GATC_y

Cuts at:      0      77     434     651 
   Size:         77     357     217

  Fragments arranged by size: 

                 357     217      77
 
BtgI C'CryG_G

Cuts at:      0       8     175     540     622     651 
   Size:          8     167     365      82      29

  Fragments arranged by size: 

                 365     167      82      29       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     402     651 
   Size:        402     249
 
EaeI y'GGCC_r

Cuts at:      0     206     651 
   Size:        206     445
 
EarI CTCTTCn'nnn_

Cuts at:      0     206     651 
   Size:        206     445
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     526     651 
   Size:        526     125
 
EcoICRI GAG'CTC

Cuts at:      0     120     269     651 
   Size:        120     149     382

  Fragments arranged by size: 

                 382     149     120
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583     651 
   Size:        583      68
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     361     651 
   Size:        361     290
 
EcoO109I rG'GnC_Cy

Cuts at:      0     476     516     558     651 
   Size:        476      40      42      93

  Fragments arranged by size: 

                 476      93      42      40
 
HaeII r_GCGC'y

Cuts at:      0     570     651 
   Size:        570      81
 
HincII GTy'rAC

Cuts at:      0     396     651 
   Size:        396     255
 
MspA1I CmG'CkG

Cuts at:      0     204     651 
   Size:        204     447
 
PasI CC'CwG_GG

Cuts at:      0     274     651 
   Size:        274     377
 
PfoI T'CCnGG_A

Cuts at:      0     254     560     651 
   Size:        254     306      91

  Fragments arranged by size: 

                 306     254      91
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     651 
   Size:        355      32     264

  Fragments arranged by size: 

                 355     264      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     516     558     651 
   Size:        516      42      93

  Fragments arranged by size: 

                 516      93      42
 
PvuII CAG'CTG

Cuts at:      0     204     651 
   Size:        204     447
 
SacI G_AGCT'C

Cuts at:      0     122     271     651 
   Size:        122     149     380

  Fragments arranged by size: 

                 380     149     122
 
SalI G'TCGA_C

Cuts at:      0     394     651 
   Size:        394     257
 
ScaI AGT'ACT

Cuts at:      0     352     651 
   Size:        352     299
 
SexAI A'CCwGG_T

Cuts at:      0     308     651 
   Size:        308     343
 
SfcI C'TryA_G

Cuts at:      0      32     651 
   Size:         32     619
 
SmlI C'TyrA_G

Cuts at:      0      73     463     651 
   Size:         73     390     188

  Fragments arranged by size: 

                 390     188      73
 
StuI AGG'CCT

Cuts at:      0     340     651 
   Size:        340     311
 
TatI w'GTAC_w

Cuts at:      0      17     350     651 
   Size:         17     333     301

  Fragments arranged by size: 

                 333     301      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      62      94     296     328     651 
   Size:         62      32     202      32     323

  Fragments arranged by size: 

                 323     202      62      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     578     651 
   Size:        578      73
 
XmnI GAAnn'nnTTC

Cuts at:      0     215     352     651 
   Size:        215     137     299

  Fragments arranged by size: 

                 299     215     137
 

 Enzymes that do cut:

     AccI     AloI    ApaLI     AvaI    BanII    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaXI    BseRI
     BsgI  BsiHKAI Bsp1286I    BsrFI    BsrGI   BstAPI    BstYI     BtgI
    BtgZI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII   HincII   MspA1I     PasI     PfoI     PpiI    PpuMI    PvuII
     SacI     SalI     ScaI    SexAI     SfcI     SmlI     StuI     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII    SanDI     SapI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI
     ZraI
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