Restriction map for pF1KE0639
 (Linear) (Six-base) MAPSORT of: KIEE0639.seq  Check: 3590  from: 1  to: 642



KIEE0639.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     642 
   Size:        433     209
 
AflIII A'CryG_T

Cuts at:      0     488     642 
   Size:        488     154
 
AhdI GACnn_n'nnGTC

Cuts at:      0     588     642 
   Size:        588      54
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     340     642 
   Size:        340     302
 
ApaI G_GGCC'C

Cuts at:      0      11     642 
   Size:         11     631
 
AvaI C'yCGr_G

Cuts at:      0     544     642 
   Size:        544      98
 
BanII G_rGCy'C

Cuts at:      0      11     439     642 
   Size:         11     428     203

  Fragments arranged by size: 

                 428     203      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     339     642 
   Size:        339     303
 
BglI GCCn_nnn'nGGC

Cuts at:      0      35     642 
   Size:         35     607
 
Bme1580I G_kGCm'C

Cuts at:      0      11     642 
   Size:         11     631
 
BmtI G_CTAG'C

Cuts at:      0     368     642 
   Size:        368     274
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     642 
   Size:        181     461
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     610     642 
   Size:        610      32
 
BsaAI yAC'GTr

Cuts at:      0      71     513     642 
   Size:         71     442     129

  Fragments arranged by size: 

                 442     129      71
 
BsaBI GATnn'nnATC

Cuts at:      0     190     642 
   Size:        190     452
 
BsaHI Gr'CG_yC

Cuts at:      0     572     642 
   Size:        572      70
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     166     642 
   Size:        166     476
 
BseYI C'CCAG_C

Cuts at:      0      14     642 
   Size:         14     628
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     642 
   Size:        244     398
 
BsiHKAI G_wGCw'C

Cuts at:      0     439     518     642 
   Size:        439      79     124

  Fragments arranged by size: 

                 439     124      79
 
BsiWI C'GTAC_G

Cuts at:      0      71     642 
   Size:         71     571
 
BsmI GAATG_Cn'

Cuts at:      0     203     642 
   Size:        203     439
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     439     518     642 
   Size:         11     428      79     124

  Fragments arranged by size: 

                 428     124      79      11
 
BsrGI T'GTAC_A

Cuts at:      0      83     642 
   Size:         83     559
 
BssSI C'ACGA_G

Cuts at:      0     110     642 
   Size:        110     532
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     381     642 
   Size:        381     261
 
BstYI r'GATC_y

Cuts at:      0     191     408     642 
   Size:        191     217     234

  Fragments arranged by size: 

                 234     217     191
 
BtgI C'CryG_G

Cuts at:      0      77     496     519     593     642 
   Size:         77     419      23      74      49

  Fragments arranged by size: 

                 419      77      74      49      23
 
DraIII CAC_nnn'GTG

Cuts at:      0     222     642 
   Size:        222     420
 
EcoICRI GAG'CTC

Cuts at:      0     437     642 
   Size:        437     205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     642 
   Size:        433     209
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7      19     642 
   Size:          7      12     623

  Fragments arranged by size: 

                 623      12       7
 
FspI TGC'GCA

Cuts at:      0     282     642 
   Size:        282     360
 
HaeII r_GCGC'y

Cuts at:      0      57     371     642 
   Size:         57     314     271

  Fragments arranged by size: 

                 314     271      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     574     606     642 
   Size:        574      32      36

  Fragments arranged by size: 

                 574      36      32
 
MspA1I CmG'CkG

Cuts at:      0      65     228     561     642 
   Size:         65     163     333      81

  Fragments arranged by size: 

                 333     163      81      65
 
NcoI C'CATG_G

Cuts at:      0     593     642 
   Size:        593      49
 
NheI G'CTAG_C

Cuts at:      0     364     642 
   Size:        364     278
 
NspI r_CATG'y

Cuts at:      0     492     642 
   Size:        492     150
 
PciI A'CATG_T

Cuts at:      0     488     642 
   Size:        488     154
 
PmlI CAC'GTG

Cuts at:      0     513     642 
   Size:        513     129
 
PpuMI rG'GwC_Cy

Cuts at:      0      19     642 
   Size:         19     623
 
PspOMI G'GGCC_C

Cuts at:      0       7     642 
   Size:          7     635
 
PvuII CAG'CTG

Cuts at:      0     228     642 
   Size:        228     414
 
SacI G_AGCT'C

Cuts at:      0     439     642 
   Size:        439     203
 
SanDI GG'GwC_CC

Cuts at:      0      19     642 
   Size:         19     623
 
SmlI C'TyrA_G

Cuts at:      0     196     642 
   Size:        196     446
 
StuI AGG'CCT

Cuts at:      0      91     642 
   Size:         91     551
 
StyI C'CwwG_G

Cuts at:      0     440     593     642 
   Size:        440     153      49

  Fragments arranged by size: 

                 440     153      49
 
TatI w'GTAC_w

Cuts at:      0      83     642 
   Size:         83     559
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AlfI     ApaI     AvaI    BanII     BbsI
     BglI Bme1580I     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI Bsp1286I    BsrGI
    BssSI   BstAPI    BstYI     BtgI   DraIII  EcoICRI  Eco57MI EcoO109I
     FspI    HaeII    Hin4I   MspA1I     NcoI     NheI     NspI     PciI
     PmlI    PpuMI   PspOMI    PvuII     SacI    SanDI     SmlI     StuI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BsaWI    BsaXI    BsiEI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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