Restriction map for pF1KE0630
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiHKAI     BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HpaI        
KpnI        Ksp632I     MamI        MfeI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AclI           66  AACGTT               AA^CGTT
AcsI          130  GAATTC               [GA]^AATT[CT]
AflIII        240  ACGCGT               A^C[GA][CT]GT
AlwI          408  GATCC                GGATC(4/5)
AlwNI         481  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          581  CCTCAGG              CC^T[ACGT]AGG
ApaI         1034  GGGCCC               GGGCC^C
ApoI          130  GAATTC               [GA]^AATT[CT]
AscI          267  GGCGCGCC             GG^CGCGCC
AviII         113  TGCGCA               TGC^GCA
AvrII         687  CCTAGG               C^CTAGG
BfaI          688  CTAG                 C^TAG
BglI          861  GCCGGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         193  AGATCT               A^GATCT
BlnI          687  CCTAGG               C^CTAGG
BsaI          160  GAGACC               GGTCTC(1/5)
BsmBI         908  GAGACG               CGTCTC(1/5)
BspMI         815  GCAGGT               ACCTGC(4/8)
BsrFI         611  ACCGGC               [GA]^CCGG[CT]
BssSI         252  CACGAG               CACGAG(-5/-1)
BstYI         193  AGATCT               [GA]^GATC[CT]
Bsu36I        581  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        611  ACCGGC               [GA]^CCGG[CT]
EaeI          859  CGGCCG               [CT]^GGCC[GA]
EagI          859  CGGCCG               C^GGCCG
EclXI         859  CGGCCG               C^GGCCG
Eco52I        859  CGGCCG               C^GGCCG
Eco81I        581  CCTCAGG              CC^T[ACGT]AGG
EcoNI         642  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         130  GAATTC               G^AATTC
FspI          113  TGCGCA               TGC^GCA
HgaI          239  GACGC                GACGC(5/10)
HindIII       214  AAGCTT               A^AGCTT
HinfI        1084  GACTC                G^A[ACGT]TC
MaeI          688  CTAG                 C^TAG
MaeIII       1024  GTCAC                ^GT[ACGT]AC
MflI          193  AGATCT               [GA]^GATC[CT]
MluI          240  ACGCGT               A^CGCGT
MslI          105  CACCTTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I       1070  CTCGAG               C^TCGAG
PflMI         465  CCACAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1084  GACTC                GAGTC(4/5)
Psp1406I       66  AACGTT               AA^CGTT
PvuII         277  CAGCTG               CAG^CTG
SfaNI         223  GATGC                GCATC(5/9)
StuI          529  AGGCCT               AGG^CCT
Tsp45I       1024  GTCAC                ^GT[GC]AC
Tsp509I       131  AATT                 ^AATT
Van91I        465  CCACAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          461  CCATCCACAGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1070  CTCGAG               C^TCGAG
XhoII         193  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          509  GTAC                 GT^AC
              819  GTAC                
AvaII         372  GGACC                G^G[AT]CC
              700  GGTCC               
BbsI          147  GAAGAC               GAAGAC(2/6)
              607  GAAGAC              
BpuAI         147  GAAGAC               GAAGAC(2/6)
              607  GAAGAC              
BsgI         1017  CTGCAC               GTGCAG(16/14)
               46  GTGCAG              
BsiEI         825  CGACCG               CG[GA][CT]^CG
              859  CGGCCG              
BsmAI         160  GAGAC                GTCTC(1/5)
              908  GAGAC               
BsrBI         885  GAGCGG               CCGCTC(-3/-3)
             1042  GAGCGG              
BssHII        268  GCGCGC               G^CGCGC
              769  GCGCGC              
BstF5I        462  CATCC                GGATG(2/0)
             1002  CATCC               
EcoT14I       663  CCAAGG               C^C[AT][AT]GG
              687  CCTAGG              
FokI          462  CATCC                GGATG(9/13)
             1002  CATCC               
HphI          405  GGTGA                GGTGA(8/7)
              922  GGTGA               
RsaI          509  GTAC                 GT^AC
              819  GTAC                
SmaI          657  CCCGGG               CCC^GGG
              929  CCCGGG              
StyI          663  CCAAGG               C^C[AT][AT]GG
              687  CCTAGG              
TaqI          221  TCGA                 T^CGA
             1071  TCGA                
TthHB8I       221  TCGA                 T^CGA
             1071  TCGA                
XmaCI         657  CCCGGG               C^CCGGG
              929  CCCGGG              
XmaI          657  CCCGGG               C^CCGGG
              929  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaI        
BanI        BanII       BbeI        BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EcoO109I    EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       ItaI        KasI        KspI        
MaeII       MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIV       PstI        SacII       
Sau3AI      Sau96I      ScrFI       SfcI        SfoI        
SmlI        TseI        TspRI       
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