Restriction map for pF1KE0630
 (Linear) (Six-base) MAPSORT of: KIEE0630.seq  Check: 8739  from: 1  to: 1113



KIEE0630.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AclI AA'CG_TT

Cuts at:      0      67    1113 
   Size:         67    1046
 
AflIII A'CryG_T

Cuts at:      0     240    1113 
   Size:        240     873
 
AleI CACnn'nnGTG

Cuts at:      0     109    1113 
   Size:        109    1004
 
AlwNI CAG_nnn'CTG

Cuts at:      0     486    1113 
   Size:        486     627
 
ApaI G_GGCC'C

Cuts at:      0    1038    1113 
   Size:       1038      75
 
ApoI r'AATT_y

Cuts at:      0     130    1113 
   Size:        130     983
 
AscI GG'CGCG_CC

Cuts at:      0     268    1113 
   Size:        268     845
 
AvaI C'yCGr_G

Cuts at:      0     657     929    1070    1113 
   Size:        657     272     141      43

  Fragments arranged by size: 

                 657     272     141      43
 
AvrII C'CTAG_G

Cuts at:      0     687    1113 
   Size:        687     426
 
BanI G'GyrC_C

Cuts at:      0     575     997    1046    1055    1113 
   Size:        575     422      49       9      58

  Fragments arranged by size: 

                 575     422      58      49       9
 
BanII G_rGCy'C

Cuts at:      0     337     383    1038    1113 
   Size:        337      46     655      75

  Fragments arranged by size: 

                 655     337      75      46
 
BbeI G_GCGC'C

Cuts at:      0    1001    1050    1059    1113 
   Size:       1001      49       9      54

  Fragments arranged by size: 

                1001      54      49       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     154     614    1113 
   Size:        154     460     499

  Fragments arranged by size: 

                 499     460     154
 
BglI GCCn_nnn'nGGC

Cuts at:      0     867    1113 
   Size:        867     246
 
BglII A'GATC_T

Cuts at:      0     193    1113 
   Size:        193     920
 
Bme1580I G_kGCm'C

Cuts at:      0     761     980    1038    1113 
   Size:        761     219      58      75

  Fragments arranged by size: 

                 761     219      75      58
 
BmgBI CAC'GTC

Cuts at:      0     564    1113 
   Size:        564     549
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      13     699    1113 
   Size:         13     686     414

  Fragments arranged by size: 

                 686     414      13
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     493     664    1060    1113 
   Size:        397      96     171     396      53

  Fragments arranged by size: 

                 397     396     171      96      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     883    1113 
   Size:        883     230
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     139    1113 
   Size:        127      12     974

  Fragments arranged by size: 

                 974     127      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154    1113 
   Size:        154     959
 
BsaHI Gr'CG_yC

Cuts at:      0     998    1047    1056    1113 
   Size:        998      49       9      57

  Fragments arranged by size: 

                 998      57      49       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     684     714     948     978    1113 
   Size:        684      30     234      30     135

  Fragments arranged by size: 

                 684     234     135      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439     661     920    1113 
   Size:        439     222     259     193

  Fragments arranged by size: 

                 439     259     222     193
 
BseYI C'CCAG_C

Cuts at:      0     870    1113 
   Size:        870     243
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67    1002    1113 
   Size:         67     935     111

  Fragments arranged by size: 

                 935     111      67
 
BsiEI CG_ry'CG

Cuts at:      0     828     862    1113 
   Size:        828      34     251

  Fragments arranged by size: 

                 828     251      34
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     902    1113 
   Size:        902     211
 
Bsp1286I G_dGCh'C

Cuts at:      0     337     383     761     980    1038    1113 
   Size:        337      46     378     219      58      75

  Fragments arranged by size: 

                 378     337     219      75      58      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     806    1113 
   Size:        806     307
 
BsrBI CCG'CTC

Cuts at:      0     887    1044    1113 
   Size:        887     157      69

  Fragments arranged by size: 

                 887     157      69
 
BsrFI r'CCGG_y

Cuts at:      0     611    1113 
   Size:        611     502
 
BssHII G'CGCG_C

Cuts at:      0     268     769    1113 
   Size:        268     501     344

  Fragments arranged by size: 

                 501     344     268
 
BssSI C'ACGA_G

Cuts at:      0     252    1113 
   Size:        252     861
 
BstYI r'GATC_y

Cuts at:      0     193    1113 
   Size:        193     920
 
Bsu36I CC'TnA_GG

Cuts at:      0     582    1113 
   Size:        582     531
 
BtgI C'CryG_G

Cuts at:      0     258     761     798     845     900    1051    1113 
   Size:        258     503      37      47      55     151      62

  Fragments arranged by size: 

                 503     258     151      62      55      47      37
 
EaeI y'GGCC_r

Cuts at:      0     859    1113 
   Size:        859     254
 
EagI C'GGCC_G

Cuts at:      0     859    1113 
   Size:        859     254
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     493     664    1060    1113 
   Size:        397      96     171     396      53

  Fragments arranged by size: 

                 397     396     171      96      53
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     646    1113 
   Size:        646     467
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7    1034    1035    1113 
   Size:          7    1027       1      78

  Fragments arranged by size: 

                1027      78       7       1
 
EcoRI G'AATT_C

Cuts at:      0     130    1113 
   Size:        130     983
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     180    1113 
   Size:        180     933
 
FspI TGC'GCA

Cuts at:      0     115    1113 
   Size:        115     998
 
HaeII r_GCGC'y

Cuts at:      0     637     878    1001    1050    1059    1113 
   Size:        637     241     123      49       9      54

  Fragments arranged by size: 

                 637     241     123      54      49       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     684     716     736     768    1113 
   Size:        684      32      20      32     345

  Fragments arranged by size: 

                 684     345      32      32      20
 
HindIII A'AGCT_T

Cuts at:      0     214    1113 
   Size:        214     899
 
KasI G'GCGC_C

Cuts at:      0     997    1046    1055    1113 
   Size:        997      49       9      58

  Fragments arranged by size: 

                 997      58      49       9
 
MluI A'CGCG_T

Cuts at:      0     240    1113 
   Size:        240     873
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     764    1113 
   Size:        263     501     349

  Fragments arranged by size: 

                 501     349     263
 
MslI CAynn'nnrTG

Cuts at:      0     109    1113 
   Size:        109    1004
 
MspA1I CmG'CkG

Cuts at:      0     260     279     698     763     800     847    1013    1113 
   Size:        260      19     419      65      37      47     166     100

  Fragments arranged by size: 

                 419     260     166     100      65      47      37      19
            
 
NarI GG'CG_CC

Cuts at:      0     998    1047    1056    1113 
   Size:        998      49       9      57

  Fragments arranged by size: 

                 998      57      49       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     471    1113 
   Size:        471     642
 
PspOMI G'GGCC_C

Cuts at:      0    1034    1113 
   Size:       1034      79
 
PspXI vC'TCGA_Gb

Cuts at:      0    1070    1113 
   Size:       1070      43
 
PstI C_TGCA'G

Cuts at:      0     278     318     500    1113 
   Size:        278      40     182     613

  Fragments arranged by size: 

                 613     278     182      40
 
PvuII CAG'CTG

Cuts at:      0     279    1113 
   Size:        279     834
 
SacII CC_GC'GG

Cuts at:      0     261     764     801     848    1113 
   Size:        261     503      37      47     265

  Fragments arranged by size: 

                 503     265     261      47      37
 
SfcI C'TryA_G

Cuts at:      0     274     314     496    1113 
   Size:        274      40     182     617

  Fragments arranged by size: 

                 617     274     182      40
 
SfoI GGC'GCC

Cuts at:      0     999    1048    1057    1113 
   Size:        999      49       9      56

  Fragments arranged by size: 

                 999      56      49       9
 
SmaI CCC'GGG

Cuts at:      0     659     931    1113 
   Size:        659     272     182

  Fragments arranged by size: 

                 659     272     182
 
SmlI C'TyrA_G

Cuts at:      0     142     154    1070    1113 
   Size:        142      12     916      43

  Fragments arranged by size: 

                 916     142      43      12
 
StuI AGG'CCT

Cuts at:      0     531    1113 
   Size:        531     582
 
StyI C'CwwG_G

Cuts at:      0     663     687    1113 
   Size:        663      24     426

  Fragments arranged by size: 

                 663     426      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     948     980    1113 
   Size:        948      32     133

  Fragments arranged by size: 

                 948     133      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     468    1113 
   Size:        468     645
 
XhoI C'TCGA_G

Cuts at:      0    1070    1113 
   Size:       1070      43
 
XmaI C'CCGG_G

Cuts at:      0     657     929    1113 
   Size:        657     272     184

  Fragments arranged by size: 

                 657     272     184
 

 Enzymes that do cut:

     AclI   AflIII     AleI    AlwNI     ApaI     ApoI     AscI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI     BglI    BglII Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI   BssHII    BssSI    BstYI   Bsu36I     BtgI     EaeI     EagI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
  HindIII     KasI     MluI     MmeI     MslI   MspA1I     NarI    PflMI
   PspOMI    PspXI     PstI    PvuII    SacII     SfcI     SfoI     SmaI
     SmlI     StuI     StyI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    ApaLI     AseI    AsiSI     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI     BmtI     BplI
    BsaAI    BsaBI    BsaWI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI
    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI
  EcoICRI    EcoRV     FseI    FspAI   HincII     HpaI     KpnI     MfeI
     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI     PsrI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI  Tth111I     XbaI     XmnI     ZraI
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