Restriction map for pF1KE0624
 (Linear) (Six-base) MAPSORT of: KIEE0624.seq  Check: 7229  from: 1  to: 1110



KIEE0624.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581    1110 
   Size:        581     529
 
AleI CACnn'nnGTG

Cuts at:      0     931    1110 
   Size:        931     179
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     375    1024    1110 
   Size:        132     243     649      86

  Fragments arranged by size: 

                 649     243     132      86
 
AvaI C'yCGr_G

Cuts at:      0     510    1110 
   Size:        510     600
 
BanI G'GyrC_C

Cuts at:      0     964    1110 
   Size:        964     146
 
BanII G_rGCy'C

Cuts at:      0     308    1110 
   Size:        308     802
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     467    1110 
   Size:        467     643
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     649     683    1043    1077    1110 
   Size:        649      34     360      34      33

  Fragments arranged by size: 

                 649     360      34      34      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     541    1110 
   Size:        541     569
 
BfrBI ATG'CAT

Cuts at:      0     149    1110 
   Size:        149     961
 
BglI GCCn_nnn'nGGC

Cuts at:      0     160    1110 
   Size:        160     950
 
Bme1580I G_kGCm'C

Cuts at:      0      82     967    1078    1110 
   Size:         82     885     111      32

  Fragments arranged by size: 

                 885     111      82      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     732    1110 
   Size:        732     378
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     729    1110 
   Size:        729     381
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1110 
   Size:        187     923
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409    1110 
   Size:        409     701
 
BsaAI yAC'GTr

Cuts at:      0     552    1110 
   Size:        552     558
 
BsaHI Gr'CG_yC

Cuts at:      0     350    1110 
   Size:        350     760
 
BseYI C'CCAG_C

Cuts at:      0       3     960    1024    1110 
   Size:          3     957      64      86

  Fragments arranged by size: 

                 957      86      64       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193    1110 
   Size:        193     917
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     524    1110 
   Size:        308     216     586

  Fragments arranged by size: 

                 586     308     216
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     308     524     967    1078    1110 
   Size:         82     226     216     443     111      32

  Fragments arranged by size: 

                 443     226     216     111      82      32
 
BspHI T'CATG_A

Cuts at:      0     473    1110 
   Size:        473     637
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401    1110 
   Size:        401     709
 
BsrBI CCG'CTC

Cuts at:      0     345    1110 
   Size:        345     765
 
BsrDI GCAATG_nn'

Cuts at:      0     149    1110 
   Size:        149     961
 
BsrFI r'CCGG_y

Cuts at:      0    1032    1110 
   Size:       1032      78
 
BsrGI T'GTAC_A

Cuts at:      0     411    1110 
   Size:        411     699
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     810    1110 
   Size:        810     300
 
BstEII G'GTnAC_C

Cuts at:      0     640    1110 
   Size:        640     470
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     933    1110 
   Size:        933     177
 
BstYI r'GATC_y

Cuts at:      0     420    1110 
   Size:        420     690
 
BtgI C'CryG_G

Cuts at:      0     820     926     999    1110 
   Size:        820     106      73     111

  Fragments arranged by size: 

                 820     111     106      73
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     603    1110 
   Size:        603     507
 
BtsI GCAGTG_nn'

Cuts at:      0      17     522    1093    1110 
   Size:         17     505     571      17

  Fragments arranged by size: 

                 571     505      17      17
 
EaeI y'GGCC_r

Cuts at:      0     137    1110 
   Size:        137     973
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      52    1110 
   Size:         52    1058
 
EcoICRI GAG'CTC

Cuts at:      0     306    1110 
   Size:        306     804
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     581    1110 
   Size:        187     394     529

  Fragments arranged by size: 

                 529     394     187
 
EcoO109I rG'GnC_Cy

Cuts at:      0      19      86     162     507     777    1110 
   Size:         19      67      76     345     270     333

  Fragments arranged by size: 

                 345     333     270      76      67      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     906     933    1110 
   Size:        906      27     177

  Fragments arranged by size: 

                 906     177      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     589     621     718     750    1110 
   Size:        448      32     109      32      97      32     360

  Fragments arranged by size: 

                 448     360     109      97      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     506    1110 
   Size:        506     604
 
MscI TGG'CCA

Cuts at:      0     139    1110 
   Size:        139     971
 
MslI CAynn'nnrTG

Cuts at:      0     931    1110 
   Size:        931     179
 
MspA1I CmG'CkG

Cuts at:      0     288     960    1039    1110 
   Size:        288     672      79      71

  Fragments arranged by size: 

                 672     288      79      71
 
NaeI GCC'GGC

Cuts at:      0    1034    1110 
   Size:       1034      76
 
NcoI C'CATG_G

Cuts at:      0     820     999    1110 
   Size:        820     179     111

  Fragments arranged by size: 

                 820     179     111
 
NgoMIV G'CCGG_C

Cuts at:      0    1032    1110 
   Size:       1032      78
 
NsiI A_TGCA'T

Cuts at:      0     151    1110 
   Size:        151     959
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     375    1024    1110 
   Size:        375     649      86

  Fragments arranged by size: 

                 649     375      86
 
PfoI T'CCnGG_A

Cuts at:      0     543    1110 
   Size:        543     567
 
PpuMI rG'GwC_Cy

Cuts at:      0     507     777    1110 
   Size:        507     270     333

  Fragments arranged by size: 

                 507     333     270
 
PstI C_TGCA'G

Cuts at:      0     959    1110 
   Size:        959     151
 
PvuII CAG'CTG

Cuts at:      0     288     960    1039    1110 
   Size:        288     672      79      71

  Fragments arranged by size: 

                 672     288      79      71
 
SacI G_AGCT'C

Cuts at:      0     308    1110 
   Size:        308     802
 
SfcI C'TryA_G

Cuts at:      0     447     582     955    1110 
   Size:        447     135     373     155

  Fragments arranged by size: 

                 447     373     155     135
 
SmaI CCC'GGG

Cuts at:      0     512    1110 
   Size:        512     598
 
SmlI C'TyrA_G

Cuts at:      0     424    1110 
   Size:        424     686
 
StuI AGG'CCT

Cuts at:      0     808    1110 
   Size:        808     302
 
StyI C'CwwG_G

Cuts at:      0     155     820     999    1110 
   Size:        155     665     179     111

  Fragments arranged by size: 

                 665     179     155     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     833    1110 
   Size:        833     277
 
TatI w'GTAC_w

Cuts at:      0     411     443    1110 
   Size:        411      32     667

  Fragments arranged by size: 

                 667     411      32
 
XbaI T'CTAG_A

Cuts at:      0     843    1110 
   Size:        843     267
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162    1110 
   Size:        162     948
 
XmaI C'CCGG_G

Cuts at:      0     510    1110 
   Size:        510     600
 
XmnI GAAnn'nnTTC

Cuts at:      0     790    1110 
   Size:        790     320
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     AvaI     BanI    BanII     BbsI     BcgI
    BciVI    BfrBI     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI
    BsaAI    BsaHI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     EaeI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NsiI    PflMI     PfoI    PpuMI     PstI    PvuII     SacI
     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BclI
    BglII     BlpI    BmgBI     BmtI   Bpu10I     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XhoI
     ZraI
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