Restriction map for pF1KE0613
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflIII      AhdI        Alw44I      
AlwI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BcnI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     CfoI        ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tsp45I      TspRI       Tth111I     Van91I      
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          498  GTCGAC               GT^[AC][GT]AC
AciI           75  GCGG                 CCGC(-3/-1)
AflII         369  CTTAAG               C^TTAAG
AgeI          486  ACCGGT               A^CCGGT
AlwNI         298  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          444  CCTGAGG              CC^T[ACGT]AGG
BanII         217  GAGCCC               G[GA]GC[CT]^C
BbvI          510  GCTGC                GCAGC(8/12)
BclI          191  TGATCA               T^GATCA
BfaI           31  CTAG                 C^TAG
BfrI          369  CTTAAG               C^TTAAG
BlpI           70  GCTCAGC              GC^T[ACGT]AGC
BmyI          217  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I       70  GCTCAGC              GC^T[ACGT]AGC
BsaWI         486  ACCGGT               [AT]^CCGG[AT]
BseRI          46  GAGGAG               GAGGAG(10/8)
BsmFI           3  GGGAC                GGGAC(10/14)
Bsp1286I      217  GAGCCC               G[AGT]GC[ACT]^C
BsrFI         486  ACCGGT               [GA]^CCGG[CT]
BstAPI        297  GCAGGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          43  CCAGAGGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        444  CCTGAGG              CC^T[ACGT]AGG
CelII          70  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        486  ACCGGT               [GA]^CCGG[CT]
DraII         299  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eco81I        444  CCTGAGG              CC^T[ACGT]AGG
EcoNI         126  CCTTGGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      299  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI          34  GAATTC               G^AATTC
EcoT14I       126  CCTTGG               C^C[AT][AT]GG
FbaI          191  TGATCA               T^GATCA
Fnu4HI        510  GCTGC                GC^[ACGT]GC
HapII         487  CCGG                 C^CGG
HincII        498  GTCGAC               GT[CT]^[GA]AC
HindII        498  GTCGAC               GT[CT]^[GA]AC
HinfI         432  GATTC                G^A[ACGT]TC
HpaII         487  CCGG                 C^CGG
HphI          130  GGTGA                GGTGA(8/7)
ItaI          510  GCTGC                GC^[ACGT]GC
MaeI           31  CTAG                 C^TAG
MaeII         227  ACGT                 A^CGT
MseI          370  TTAA                 T^TAA
MspA1I         73  CAGCGG               C[AC]G^C[GT]G
MspI          487  CCGG                 C^CGG
NlaIV          63  GGACCC               GG[ACGT]^[ACGT]CC
NspI          468  GCATGT               [GA]CATG^[CT]
PinAI         486  ACCGGT               A^CCGGT
SalI          498  GTCGAC               G^TCGAC
SfaNI         327  GATGC                GCATC(5/9)
SfcI          108  CTACAG               C^T[GA][CT]AG
StuI          448  AGGCCT               AGG^CCT
StyI          126  CCTTGG               C^C[AT][AT]GG
TfiI          432  GATTC                G^A[AT]TC
Tru9I         370  TTAA                 T^TAA
TseI          510  GCTGC                G^C[AT]GC
XbaI           30  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI           34  GAATTC               [GA]^AATT[CT]
              386  AAATTC              
ApoI           34  GAATTC               [GA]^AATT[CT]
              386  AAATTC              
AvaII          63  GGACC                G^G[AT]CC
              489  GGTCC               
BpmI           41  CTCCAG               CTGGAG(16/14)
              202  CTGGAG              
BsaJI         126  CCTTGG               C^C[ACGT][ACGT]GG
              220  CCCTGG              
BsiYI         126  CCTTGGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              292  CCAGAGCAGGG         
BslI          126  CCTTGGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              292  CCAGAGCAGGG         
BsmI           55  GAATGC               GAATGC(1/-1)
               13  GCATTC              
BsrI          380  ACTGG                ACTGG(1/-1)
              492  CCAGT               
BssKI          60  CCTGG                ^CC[ACGT]GG
              221  CCTGG               
BstF5I         21  GGATG                GGATG(2/0)
               90  GGATG               
BstNI          60  CCTGG                CC^[AT]GG
              221  CCTGG               
Cac8I         302  GCCTGC               GC[ACGT]^[ACGT]GC
              405  GCTTGC              
DpnII         120  GATC                 ^GATC
              192  GATC                
EcoRII         60  CCTGG                ^CC[AT]GG
              221  CCTGG               
FokI           21  GGATG                GGATG(9/13)
               90  GGATG               
HaeIII        301  GGCC                 GG^CC
              449  GGCC                
MboI          120  GATC                 ^GATC
              192  GATC                
MvaI           60  CCTGG                CC^[AT]GG
              221  CCTGG               
MwoI          297  GCAGGGCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              468  GCATGTAAAGC         
NdeII         120  GATC                 ^GATC
              192  GATC                
Sau3AI        120  GATC                 ^GATC
              192  GATC                
ScrFI          60  CCTGG                CC^[ACGT]GG
              221  CCTGG               
SmlI          355  CTTGAG               C^T[CT][GA]AG
              369  CTTAAG              
TaqI          499  TCGA                 T^CGA
              506  TCGA                
TthHB8I       499  TCGA                 T^CGA
              506  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BbsI        BpuAI       Cfr13I      DdeI        
MboII       MnlI        NlaIII      Sau96I      Tsp509I     
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